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931.
A sensitive electrochemiluminescence (ECL) biosensor for the specific DNA sequence of hepatitis C virus (HCV) was developed based on the efficient quenching effect of the ferrocene cluster functionalized gold nanoparticles (Fc@AuNPs) on the ECL of electrodeposited silica@Ru(bpy)32+-chitosan-graphene oxide nanocomposite (SiO2@Ru−CS−GO). Graphene oxide (GO) can accelerate electron transfer rate, thus improving the ECL of Ru(bpy)32+ on electrode surface. The molecular beacons (MB) was fixed to SiO2@Ru−CS−GO by glutaraldehyde (GA) using the Schiff reaction between amino groups of chitosan (CS) and MB. The ECL of SiO2@Ru−CS−GO was depressed greatly by the Fc@AuNPs labelled at the end of MB, then, a stronger ECL was observed when the distance between Fc@AuNPs and SiO2@Ru−CS−GO increased after the hybridization of target DNA with MB. Under optimum conditions, the restored ECL intensity increased linearly with the target DNA concentration in the range of 1.0×10−16∼1.0×10−10 mol ⋅ L−1, and the limit of detection (LOD) is 1.4×10−17 mol ⋅ L−1. The proposed method exhibits acceptable stability and reproducibility. In general, the constructed HCV biosensor can be used for the sensitive detection of HCV in human serum, suggesting potential application prospects in bioanalysis. 相似文献
932.
Ying Xi Alexandria L. Sohn Alena N. Joignant Stephanie M. Cologna Boone M. Prentice David C. Muddiman 《Journal of mass spectrometry : JMS》2023,58(2):e4836
Mass spectrometry imaging (MSI) is an important analytical technique that simultaneously reports the spatial location and abundance of detected ions in biological, chemical, clinical, and pharmaceutical studies. As MSI grows in popularity, it has become evident that data reporting varies among different research groups and between techniques. The lack of consistency in data reporting inherently creates additional challenges in comparing intra- and inter-laboratory MSI data. In this tutorial, we propose a unified data reporting system, SMART, based on the common features shared between techniques. While there are limitations to any reporting system, SMART was decided upon after significant discussion to more easily understand and benchmark MSI data. SMART is not intended to be comprehensive but rather capture essential baseline information for a given MSI study; this could be within a study (e.g., effect of spot size on the measured ion signals) or between two studies (e.g., different MSI platform technologies applied to the same tissue type). This tutorial does not attempt to address the confidence with which annotations are made nor does it deny the importance of other parameters that are not included in the current SMART format. Ultimately, the goal of this tutorial is to discuss the necessity of establishing a uniform reporting system to communicate MSI data in publications and presentations in a simple format to readily interpret the parameters and baseline outcomes of the data. 相似文献
933.
Helian Li Yongxin Zhang Xiaoxiao Yang Zhenxi Deng Zhimin Zhu Pan Zhou Xinke Ouyang Yuting Yuan Xi Chen Lingyue Yang Meng Liu Prof. Dr. Chao Shu 《Angewandte Chemie (International ed. in English)》2023,62(15):e202300159
Despite the significance of sultines in synthesis, medicine, and materials science, the chemistry of sultines has remained unexplored due to their inaccessibility. Herein, we demonstrate the development of a photoredox-catalyzed multifluoromethyl radical addition/SO2 incorporation/polar cyclization cascade approach to multifluoromethylated γ-sultines. The reactions proceed by single electron transfer induced multifluoromethyl radical addition to an alkene followed by SO2 incorporation, and single-electron reduction for polar 5-exo-tet cyclization. Key to the success of the protocol is the use of easily oxidizable multifluoroalkanesulfinates as bifunctional reagents. The reactions proceed with excellent functional-group tolerance to deliver γ-sultines in moderate to excellent yields. 相似文献
934.
Shuang Qiu Wannan Li Tao Deng Angzhi Bi Yang Yang Xi Jiang Dr. Jie P. Li 《Angewandte Chemie (International ed. in English)》2023,62(28):e202303014
Intercellular proximity labeling has emerged as a promising approach to enable the study of cell-cell interactions (CCIs), but the efficiency of current platforms is limited. Here, we use Ru(bpy)32+ to construct an efficient photocatalytic proximity labeling (PPL) system on the cell surface that allows the highly discriminative CCI detection with spatiotemporal resolution. Through the mechanism study and quantitative characterization on living cells, we demonstrate that the singlet-oxygen (1O2) mechanism is more efficient and specific than the single electron transfer (SET) mechanism in Ru-mediated PPL. Ru(bpy)32+ catalysts with different cell-anchoring moieties are prepared to facilitate the catalyst loading on primary cells. Finally, based on this system, we develop a “live” T cell receptor (TCR) multimer with TCR-T cells that could sensitively identify and discriminate cells presenting antigens of different affinity, providing a powerful tool to better understand the heterogeneity of antigen presenting cells. 相似文献
935.
Pei Chen Xi Su Chuanzhe Wang Dr. Guang Zhang Dr. Ting Zhang Prof. Gang Xu Prof. Long Chen 《Angewandte Chemie (International ed. in English)》2023,62(40):e202306224
The emergence of two-dimensional conjugated metal–organic frameworks (2D c-MOFs) with pronounced electrical properties (e.g., high conductivity) has provided a novel platform for efficient energy storage, sensing, and electrocatalysis. Nevertheless, the limited availability of suitable ligands restricts the number of available types of 2D c-MOFs, especially those with large pore apertures and high surface areas are rare. Herein, we develop two new 2D c-MOFs (HIOTP-M, M=Ni, Cu) employing a large p-π conjugated ligand of hexaamino-triphenyleno[2,3-b:6,7-b′:10,11-b′′]tris[1,4]benzodioxin (HAOTP). Among the reported 2D c-MOFs, HIOTP-Ni exhibits the largest pore size of 3.3 nm and one of the highest surface areas (up to 1300 m2 g−1). As an exemplary application, HIOTP-Ni has been used as a chemiresistive sensing material and displays high selective response (405 %) and a rapid response (1.69 min) towards 10 ppm NO2 gas. This work demonstrates significant correlation linking the pore aperture of 2D c-MOFs to their sensing performance. 相似文献
936.
The optical transient and kinetics characterizations of the transients formed in the reaction of OH with benzotrifluoride (BTF) were performed by a laser flash photolysis technique. The results indicated that the formation of π‐type adduct of C6H5(OH)CF3 was the major reaction channel, and the δ‐type adduct of C6H5CF3OH formation was an additional minor process in the oxidation reaction of BTF attacked by OH radicals yielded from the photolysis of H2O2. Addition of OH to the CF3 group led to the fluoride ion elimination to yield α,α‐difluorophenylcarbinol (C6H5CF2OH). Trifluoromethylphenol (HOC6H4CF3) of meta‐, para‐ and ortho‐substituted isomers resulted from the addition of OH to the BTF aromatic ring. 相似文献
937.
通过电子显微镜观察了阴离子gemini表面活性剂C11- p-PhCNa和阳离子传统表面活性剂DTAB混合体系双水相中囊泡形貌随体系组成和浓度的转变。结果表明,双水相较浓的一相中形成了多层囊泡,囊泡的大小和壁厚随相的组成和浓度而改变,两组分等电荷混合有利于形成较大且壁较厚的囊泡。分析表明, gemini表面活性剂在聚集体中采取的反式构象可能是其容易形成厚壁多层囊泡的重要原因,C11- p-PhCNa联接链上的苯氧基与DTA+之间的p-阳离子相互作用以及两组分相反电性头基之间的静电吸引使囊泡壁的多层结构更加稳定。 相似文献
938.
通过水热方法合成了一种新颖的异金属金属-异烟酸无机-有机杂化体[Zn0.5(H2O)]{(Hg2Cl5)[Er(C6NO2H4)3(H2O)2]}(HgCl2)·0.5CH3OH·0.5H2O (1)并对其进行了单晶X-射线衍射结构表征。该化合物是首例4f-3d-5d多金属-异烟酸配合物。化合物1属于单斜晶系C2/c空间群,每个单胞中有8个分子,晶体学参数为:a = 34.165(4) Å,b = 9.4692(8) Å,c = 24.575(3) Å,β = 115.090(5)°,V = 7200(1) Å3,C18.50H21Cl7ErHg3N3O10Zn0.50,Mr = 1495.25,Dc = 2.759 g/cm3,T = 293(2) K,μ(MoKα) = 15.954 mm-1,F(000) = 5400, R1/wR2 = 0.0561/0.0909,共有6468个独立衍射点,其中[I > 2σ(I)]的有3157个。该化合物具有新颖的二维{(Hg2Cl5)[Er(C6NO2H4)3(H2O)2]}层状结构,由Hg2Cl5–连接[Er(C6NO2H4)3(H2O)2]链形成。该二维层和氯化汞及结晶水之间通过氢键形成三维结构,甲醇分子和水合锌离子位于该三维结构的空隙中。对化合物1的磁性测试显示该化合物具有反铁磁作用。 相似文献
939.
本文建立了一种快速的微薄辅助萃取-高效液相色谱法分离检测羊肝、牛肉和牛奶样品中三种喹诺酮类药物(氟罗沙星、洛美沙星和斯帕沙星)和两种非甾类化合物(酮洛芬和布洛芬)的方法。并对微波辅助萃取条件利用正交试验进行了优化:萃取温度为4℃、萃取时间为6min、萃取溶剂为乙腈、萃取溶剂体积为10mL。在浓度范围为0.25—0.75 μg•g-1时各待测物的三种不同水平的添加回收率在60.0%到107%之间,并获得较好的精确度(RSD<11%)。 相似文献
940.