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91.
Josep L. Acero Sanchez Olivier Y. F. Henry Teresa Mairal Nadja Laddach Anders Nygren Siegfried Hauch Jasmin Fetisch Ciara K. O’Sullivan 《Analytical and bioanalytical chemistry》2010,397(6):2325-2334
An enzyme-linked oligonucleotide assay (ELONA) for quantification of mRNA expression of five genes involved in breast cancer,
extracted from isolated rare tumour cells and amplified by multiplex ligation-dependent probe amplification (MLPA) is presented.
In MLPA, a multiplex oligonucleotide ligation assay is combined with a PCR reaction in which all ligation products are amplified
by use of a single primer pair. Biotinylated probes complementary to each of the target sequences were immobilised on the
surface of a streptavidin-coated microtitre plate and exposed to single-stranded MLPA products. A universal reporting probe
sequence modified with horseradish peroxidase (URP–HRP) and complementary to a universal primer used during the MLPA step
was further added to the surface-bound duplex as a reporter probe. Simultaneous addition of anchoring probe and target, followed
by addition of reporter probe, rather than sequential addition, was achieved with no significant effect on sensitivity and
limits of detection, but considerably reduced the required assay time. Detection limits as low as 20 pmol L−1, with an overall assay time of 95 min could be achieved with negligible cross-reactivity between probes and non-specific
targets present in the MLPA-PCR product. The same MLPA-PCR product was analysed using capillary electrophoresis, the technique
typically used for analysis of MLPA products, and good correlation was observed. The assay presented is easy to carry out,
relatively inexpensive, rapid, does not require sophisticated instrumentation, and enables quantitative analysis, making it
very promising for the analysis of MLPA products. 相似文献
92.
Using an empirical model for the density of states functions associated with hydrogenated amorphous silicon, with defect states taken into account, we examine how the distributions of such states shape the optical response of this material. The contributions to this response attributable to the various types of optical transitions are also determined. Finally, we demonstrate that we are able to capture the spectral dependence of the optical absorption coefficient associated with a defect absorption influenced sample of hydrogenated amorphous silicon using our empirical formalism for the density of states functions associated with this material. 相似文献
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96.
Hao Liu Fuyou Liang Jasmin Wong Takashi Fujiwara Wenjing Ye Ken-iti Tsubota Michiko Sugawara 《Acta Mechanica Sinica》2015,31(4):446-464
The human cardiovascular system is a closedloop and complex vascular network with multi-scaled heterogeneous hemodynamic phenomena. Here, we give a selective review of recent progress in macro-hemodynamic modeling, with a focus on geometrical multi-scale modeling of the vascular network, micro-hemodynamic modeling of microcirculation, as well as blood cellular, subcellular,endothelial biomechanics, and their interaction with arterial vessel mechanics. We describe in detail the methodology of hemodynamic modeling and its potential applications in cardiovascular research and clinical practice. In addition,we present major topics for future study: recent progress of patient-specifi hemodynamic modeling in clinical applications, micro-hemodynamic modeling in capillaries and blood cells, and the importance and potential of the multi-scale hemodynamic modeling. 相似文献
97.
Jasmin Hannighofer Marja Van den Heuvel-Panhuizen Sebastian Weirich Alexander Robitzsch 《ZDM》2011,43(5):651-665
The focus of this study was to investigate primary school students’ achievement in the domain of measurement. We analyzed a large-scale data set (N = 6,638) from German third and fourth graders (8- to 10-year-olds). These data were collected in 2007 within the framework of the ESMaG (Evaluation of the Standards in Mathematics in Primary School) project carried out by the Institute for Educational Quality Improvement (IQB) at Humboldt University, Berlin, Germany. The data were interpreted using a classification scheme based on a conceptual–procedural distinction in measurement competence. The analyses with this classification revealed that grade, gender, and in particular figural reasoning ability are significantly related to overall measurement competence as well as on the sub-competencies of Instrumental knowledge and Measurement sense. The paper concludes with a discussion of the implications of the findings of this study for teaching and assessing measurement. 相似文献
98.
Kelly TR Cai X Damkaci F Panicker SB Tu B Bushell SM Cornella I Piggott MJ Salives R Cavero M Zhao Y Jasmin S 《Journal of the American Chemical Society》2007,129(2):376-386
Building on prototype 1, which achieves 120 degrees of phosgene-powered unidirectional rotation to rotamer 6 (see Figure 5 in the full article), 7 was designed to accomplish repeated unidirectional rotation (see Scheme 7). Compound 7 contains an amino group on each blade of the triptycene and a 4-(dimethylamino)pyridine (DMAP) unit to selectively deliver phosgene (or its equivalent) to the amine in the "firing position". The synthesis of 7 is described: the key constructive steps are a benzyne addition to an anthracene to generate the triptycene, a stilbene photocyclization to construct the helicene, and a Stille coupling to incorporate the DMAP unit. The DMAP unit was shown to regioselectively relay 1,1'-carbonyldiimidazole (but not phosgene) to the proximal amino group, as designed, but rotation of the triptycene does not occur. Extensive attempts to troubleshoot the problem led to the conclusion that the requisite intramolecular urethane formation, as demonstrated in the prototype (1 --> 4), does not occur with 7 (to give 85) or 97 (to give 100). We speculate that either (i) hydrogen bonding between the hydroxypropyl group and functionality present in 7 but absent from 1 or (ii) a Bürgi-Dunitz (or similar) interaction involving the DMAP (see 106) prevents achievement of a conformation conducive to intramolecular urethane formation. 相似文献
99.
Martin Pabst Stephan Rupert Fagerer Rudolf Köhling Klaus Eyer Jasmin Krismer Konstantins Jefimovs Alfredo Jesus Ibáñez Renato Zenobi 《Journal of the American Society for Mass Spectrometry》2014,25(6):1083-1086
Drug monitoring is usually performed by liquid chromatography coupled with optical detection or electrospray ionization mass spectrometry. More recently, matrix-assisted laser desorption/ionization (MALDI) in combination with triple quadrupole or Fourier-transform (FT) mass analyzers has also been reported to allow accurate quantification. Here, we present a strategy that employs standard MALDI time-of-flight (TOF) mass spectrometry (MS) for the sensitive and accurate quantification of saquinavir from an extract of blood peripheral mononuclear cells. Unambiguous identification of saquinavir in the mass spectra was possible because of using internal mass calibration and by an overall low chemical noise in the low mass range. Exact mass determination of the constant background peaks of the cell extract, which were used for recalibration, was performed by an initial MALDI-FT-MS analysis. Fast and multiplexed sample analysis was enabled by microarray technology, which provided 10 replicates in the lower nL range for each sample in parallel lanes on a chip. In order to validate the method, we employed various statistical tests, such as confidence intervals for linear regressions, three quality control samples, and inverse confidence limits of the estimated concentration ratios. Figure
? 相似文献
100.
Joan Simó Padial Dr. René de Gelder Dr. Célia Fonseca Guerra Prof. Dr. F. Matthias Bickelhaupt Dr. Jasmin Mecinović 《Chemistry (Weinheim an der Bergstrasse, Germany)》2014,20(21):6268-6271
The through‐space polar–π interactions between pyridinium ion and the adjacent aromatic rings in 2,6‐diarylpyridines affect the pKa values. Hammett analysis illustrates that the basicity of pyridines correlates well with the sigma values of the substituents at the para position of the flanking aryl rings. 相似文献