全文获取类型
收费全文 | 224篇 |
免费 | 12篇 |
国内免费 | 1篇 |
专业分类
化学 | 142篇 |
晶体学 | 1篇 |
力学 | 3篇 |
数学 | 52篇 |
物理学 | 39篇 |
出版年
2023年 | 1篇 |
2022年 | 5篇 |
2021年 | 2篇 |
2020年 | 2篇 |
2019年 | 5篇 |
2018年 | 8篇 |
2017年 | 4篇 |
2016年 | 10篇 |
2015年 | 11篇 |
2014年 | 11篇 |
2013年 | 17篇 |
2012年 | 24篇 |
2011年 | 32篇 |
2010年 | 8篇 |
2009年 | 10篇 |
2008年 | 17篇 |
2007年 | 9篇 |
2006年 | 14篇 |
2005年 | 7篇 |
2004年 | 6篇 |
2003年 | 6篇 |
2002年 | 7篇 |
2001年 | 3篇 |
2000年 | 1篇 |
1999年 | 1篇 |
1998年 | 1篇 |
1997年 | 2篇 |
1996年 | 1篇 |
1995年 | 1篇 |
1994年 | 2篇 |
1993年 | 4篇 |
1992年 | 1篇 |
1986年 | 1篇 |
1984年 | 1篇 |
1982年 | 1篇 |
1980年 | 1篇 |
排序方式: 共有237条查询结果,搜索用时 15 毫秒
101.
A new lab-on-a-chip compatible binding assay platform is introduced. The platform combines dry-chemistry bioaffinity reagents and the recently introduced ArcDia TPX binding assay technique. The technique employs polymer microspheres as a solid phase reaction carrier, fluorescently labeled antibody conjugates, and detection of fluorescence emission from the surface of individual microspheres by two-photon excitation fluorescence. Signal response of the technique is independent of the reaction volume, thus the technique is particularly well suited for detection of bioaffinity reactions from miniature volumes. Performance of the new assay platform is studied by means of an immunometric assay of human alpha-fetoprotein (hAFP) in 384-plate format, and the results are compared to those of a corresponding wet-chemistry assay method. The results show that the ArcDia TPX detection technique can be combined with dry-chemistry reagents without compromises in assay performance. The microchip field has so far been characterized with a lack of microchip-compatible detection platforms which would allow cost-effective microchip design and sensitive bioaffinity detection. The presented detection technique is expected to provide a solution for this shortage. 相似文献
102.
A. I. Martikainen 《Journal of Mathematical Sciences》1982,20(3):2214-2221
Necessary and sufficient conditions are found for relations of the type of the law of the iterated logarithm expressed in terms of exponential moments of independent terms.Translated from Zapiski Nauchnykh Seminarov Leningradskogo Otdeleniya Matematicheskogo Instituta im. V. A. Steklova AN SSSR, Vol. 85, pp. 158–168, 1979. 相似文献
103.
Morphology of dry lignins and size and shape of dissolved kraft lignin particles by X-ray scattering 总被引:1,自引:0,他引:1
Vainio U Maximova N Hortling B Laine J Stenius P Simola LK Gravitis J Serimaa R 《Langmuir : the ACS journal of surfaces and colloids》2004,20(22):9736-9744
Lignin is a highly branched polymer consisting of phenylpropane units, and it is one of the ingredients of the supporting matrix in plant cell walls. The morphology of several lignins extracted from plant cell walls using different methods was studied by small-angle and ultra-small-angle X-ray scattering. A power-law type intensity was observed for the dry lignins, but on the basis of the power-law exponent the fractal approach often applied to lignins is not fully justified. However, the intensity of kraft lignin did show a power law with surface fractal dimension D(s) = 2.7 +/- 0.1. The specific surface area of the lignins ranged from about 0.5 to 60 m(2)/g with 20% relative accuracy. The radius of gyration was determined from small-angle X-ray scattering data for aqueous solutions of kraft lignin. The shape of the particles in NaCl and NaOH solutions was found to be elongated. The particles were about 1-3 nm thick, while the length (5-9 nm) depended on the solvent and on the lignin concentration. The size of these primary particles was approximately the same as the size of the pores in the fractal aggregates of the dry kraft lignin. Their size was determined to be about 3.5 nm. 相似文献
104.
105.
106.
107.
108.
109.
The influence of addition of small amounts of either citric acid or lactic acid on the formation of crystalline matter in
dried gels derived from a multi-component industrial sol–gel silica precursor has been studied. The sols were water-based
and had formic acid as the main acid constituent. A pronounced decrease in the extent of crystallization was observed for
both acids, with citric acid being more effective than lactic acid. The results are discussed based on the complexation behavior
of the corresponding acids under the studied conditions, and the complexation behavior in solution can be directly linked
to the extent of crystallization in the dried gels. However, the sol–gel kinetics followed that expected for a purely silica-based
sol, which suggests that the kinetics is mainly controlled by the silica portion of the sol. The results are suggested to
be of importance for the industrial use of these sols as binders, as pronounced crystallization in the gels upon drying may
lead to mechanical stresses, and thus to a decreased binder performance. 相似文献
110.
Vehniäinen MarkusLeivo Janne Lamminmäki Urpo 《Trends in analytical chemistry : TRAC》2011,30(2):219-226
The use of antibodies in immunodiagnostics has achieved new insights with recombinant technologies. This review summarizes the methods used to produce recombinant antibodies and those to tailor their properties. Finally, we address the advantages and the possibilities of recombinant antibodies in immunoanalytical applications through examples with the main focus on applications related to food quality and safety analysis. 相似文献