The characterisation of a laboratory quality control material (QCM) for dibutyltin (DBT) and tributyltin (TBT) in sewage sludge
is described. The reference values were determined by the use of two different types of isotope-dilution mass spectrometry:
gas chromatography–mass spectrometry and gas chromatography–inductively coupled plasma mass spectrometry. To avoid possible
analytical errors such as non-quantitative extraction and species degradation during sample preparation, different extraction
methods were tested (microwave- and ultrasound-assisted extraction and mechanical stirring). The reference values were based
on the unweighted means of results from the homogenisation and characterisation studies. The reference values obtained were
1,553 ± 87 and 534 ± 38 ng Sn g-1 for DBT and TBT, respectively. In the uncertainty budget estimation, the sample inhomogeneity and between-method imprecision
were taken into account. The concentrations of DBT and TBT in QCM are similar to those in the harbour sediment certified reference
material PACS-2. Likewise, the levels of DBT and TBT are in the range of these compounds normally present in sewage sludge
worldwide. In the future, the QCM will be used for an intercomparison study on DBT and TBT in sewage sludge, and as a day-to-day
QCM during studies concerning the application of sewage sludge as an additive to artificial soil or as a raw material in civil
engineering construction. 相似文献
Carr-Purcell-Meiboom-Gill train of radiofrequency pulses applied to spins in the constant magnetic field gradient is an efficient variant of the modulated magnetic field gradient spin echo method, which provides information about molecular diffusion in the frequency-domain instead in the time-domain as with the two-pulse gradient spin echo. The frequency range of novel technique is broad enough to sample the power spectrum of displacement fluctuation in water-saturated pulverized silica (SiO(2)) and provides comprehensive information about the molecular restricted motion as well as about the structure of medium. 相似文献
The modulation of spin phase produced by Carr-Purcell-Meiboom-Gill (CPMG) sequence in combination with constant magnetic field gradient is appropriate to probe the displacement power spectrum (DPS). The spin-echo attenuation is directly proportional to the DPS value at the applied modulation frequency. Relaxation and selective excitation effects can be factored out while probing the DPS. The modulation frequency is adjusted by varying the pulse separation time while the gradient strength and the time of acquisition are kept constant. In designing the experiment gradient strength limitations, imposed by off-resonance effects, as well as limitations arising from using Gaussian phase approximation must be considered. An effective experimental strategy is presented, supported by experimental results for free and restricted diffusion. 相似文献
Ozonation of various silanes and germanes produced the corresponding hydrotrioxides, R3SiOOOH and R3GeOOOH, which were characterized by 1H, 13C, 17O, and 29Si NMR, and by infrared spectroscopy in a two-pronged approach based on measured and calculated data. Ozone reacts with the E-H (E = Si, Ge) bond via a concerted 1,3-dipolar insertion mechanism, where, depending on the substituents and the environment (e.g., acetone-d6 solution), the H atom transfer precedes more and more E-O bond formation. The hydrotrioxides decompose in various solvents into the corresponding silanols/germanols, disiloxanes/digermoxanes, singlet oxygen (O2(1delta(g))), and dihydrogen trioxide (HOOOH), where catalytic amounts of water play an important role as is indicated by quantum chemical calculations. The formation of HOOOH as a decomposition product of organometallic hydrotrioxides in acetone-d6 represents a new and convenient method for the preparation of this simple, biochemically important polyoxide. By solvent variation, singlet oxygen (O2(1delta(g))) can be generated in high yield. 相似文献
This paper presents a new hybrid global optimization method referred to as DESA. The algorithm exploits random sampling and
the metropolis criterion from simulated annealing to perform global search. The population of points and efficient search
strategy of differential evolution are used to speed up the convergence. The algorithm is easy to implement and has only a
few parameters. The theoretical global convergence is established for the hybrid method. Numerical experiments on 23 mathematical
test functions have shown promising results. The method was also integrated into SPICE OPUS circuit simulator to evaluate
its practical applicability in the area of analog integrated circuit sizing. Comparison was made with basic simulated annealing,
differential evolution, and a multistart version of the constrained simplex method. The latter was already a part of SPICE
OPUS and produced good results in past research. 相似文献
Quercetin (QUE) is a well-known natural product that can exert beneficial properties on human health. However, due to its low solubility its bioavailability is limited. In the present study, we examine whether its formulation with two cyclodextrins (CDs) may enhance its pharmacological profile. Comparative interaction studies of quercetin with 2-hydroxyl-propyl-β-cyclodextrin (2HP-β-CD) and 2,6-methylated cyclodextrin (2,6Me-β-CD) were performed using NMR spectroscopy, DFT calculations, and in silico molecular dynamics (MD) simulations. Using T1 relaxation experiments and 2D DOSY it was illustrated that both cyclodextrin vehicles can host quercetin. Quantum mechanical calculations showed the formation of hydrogen bonds between QUE with 2HP-β-CD and 2,6Μe-β-CD. Six hydrogen bonds are formed ranging between 2 to 2.8 Å with 2HP-β-CD and four hydrogen bonds within 2.8 Å with 2,6Μe-β-CD. Calculations of absolute binding free energies show that quercetin binds favorably to both 2,6Me-β-CD and 2HP-β-CD. MM/GBSA results show equally favorable binding of quercetin in the two CDs. Fluorescence spectroscopy shows moderate binding of quercetin in 2HP-β-CD (520 M−1) and 2,6Me-β-CD (770 M−1). Thus, we propose that both formulations (2HP-β-CD:quercetin, 2,6Me-β-CD:quercetin) could be further explored and exploited as small molecule carriers in biological studies. 相似文献
DNA sequences containing at least four runs of repetitive cytosines can fold into tetra-helical structures called i-Motifs (iMs). The interest in these DNA secondary structures is increasing due to their therapeutical and technological applications. Still, limited knowledge of their folding requirements is currently available. We developed a novel step-by-step pipeline for the systematic screening of putative iM-forming model sequences. Focusing on structures comprising only three cytosine-cytosine+ base pairs, we investigated what the minimal lengths of the loops required for formation of an intra-molecular iM are. Our data indicate that two and three nucleotides are required to connect the strands through the minor and majorgrooves of the iM, respectively. Additionally, they highlight an asymmetric behavior according to the distribution of the cytosines. Specifically, no sequence containing a single cytosine in the first and third run was able to fold into intra-molecular iMs with the same stability of those formed when the first and the third run comprise two cytosines. This knowledge represents a step forward toward the development of prediction tools for the proper identification of biologically functional iMs, as well as for the rational design of these secondary structures as technological devices. 相似文献
By suitably adjusting the tropical algebra technique we compute the rainbow independent domination numbers of several infinite families of graphs including Cartesian products \(C_n \Box P_m\) and \(C_n \Box C_m\) for all n and \(m\le 5\), and generalized Petersen graphs P(n, 2) for \(n \ge 3\).
The paper provides a short history of the operations research (OR) in Slovenia. Some major events and achievements are mentioned and briefly discussed. The period starts in the year 1964, with the first symposium on OR in Slovenia. In the following decades, there were several important milestones: (1) the start of master’s and Ph.D. studies in OR in 1974, (2) the establishment of SSI-SSOR in 1992 (SSI-SSOR stands for the Slovenian Section for Operational Research within the Slovenian Society of Informatics), and (3) the start of a series of symposia in operations research in 1993. All these activities resulted in an extensive list of publications, projects, and monographs and international connections, proving that OR is still a vibrant field, which facilitates knowledge transfer from pure research to business applications.
Central European Journal of Operations Research - The measurement of electricity consumption at 15-minute granularity, including for households, is increasingly mandated in the EU and this also... 相似文献