CeO2-deposited mesoporous silica nanoparticles were synthesized as a probe to determine carcinoembryonic antigen (CEA) in serum by inductively coupled plasma-mass spectrometry (ICP-MS). The prepared mesoporous nanoparticles were modified and tagged to the target for sandwich-type immunoassay. Fe3O4 magnetic nanoparticles (MNPs) were also synthesized and immobilized with antibody to extract the target biomarker. The calibration curve of the synthesized CeO2-deposited silica nanoparticles, which was plotted by the signal ratio of 140Ce/57Fe measured by ICP-MS vs. the concentration of CEA, showed excellent linearity and sensitivity owing to the signal amplification and low spectral interference. Under optimal conditions, the sandwich-type analytical method was applied to determine CEA in serum spiked in the range of 0.001–5 ng mL−1 and showed a limit of detection of 0.36 ng mL−1. Since the deposited CeO2 in the mesoporous silica layer can be substituted by other metal compounds, various kinds of metal-deposited nanoparticles can be prepared as probe materials for multiplex detection in bioanalysis. 相似文献
A non-suppressed contactless conductivity detector has been used as a capillary detector in a capillary ion chromatograph, combining a reversed-phase C30 column permanently modified with ionic surfactant. The C30 column (100 × 0.32 mm. id) was modified with sodium dodecyl sulfate (SDS) for the separation of inorganic cations. Monovalent cations could be separated by the proposed system, in which methanesulfonic acid (MSA) and SDS were employed as the mobile phase component, but divalent cations could not be eluted under this condition. As for the case of SDS used as the eluent, an H+-cation-exchange column was placed before the sample injector to convert the Na+ from the eluent into H+, and when the mixture of MSA and dodecyl sulfuric acid was used as the eluent, the retention of cations was improved and baseline separation of the cations was achieved within 23 min. The effect of the eluent composition on the retention behavior of inorganic cations was investigated. The repeatability of retention time and peak height varied from 0.39 to 0.58 and 2.21 to 3.25 % as relative standard deviation, respectively.
A novel, functional‐group‐tolerant, and highly regioselective one‐pot synthesis of six 4‐chloro‐1‐aryl‐3‐oxypyrazoles, 8a – 8f , containing an oximino ester moiety has been developed. Their structures were characterized by 1H‐ and 13C‐NMR, IR, MS, and elemental analyses. The regioselectivity of the reaction was also determined by single‐crystal X‐ray diffraction analysis of product 8d . The reaction pathway, proposed with the aid of DFT calculations, likely proceeds via a DMF‐catalyzed mechanism, which involves an electrophilic attack by SOCl2 and two nucleophilic substitutions by benzyl bromide (BnBr) and Cl?, respectively, as the key steps. A preliminary in vitro bioassay indicated that most compounds exhibited good fungicidal activities against Sclerotinia sclerotiorum and Gibberella zeae. Especially, 8d and 8e displayed higher or similar fungicidal activities compared with pyraclostrobin at the concentration of 10 μg/ml. 相似文献
A series of novel 6H‐1,3,5‐oxathiazine S,S‐dioxides were synthesized by the m‐CPBA oxidation (2.2 equiv) of 6H‐1,3,5‐oxathizines. The synthetic utilities of the newly synthesized cyclic sulfones were investigated. In a thermal condition, compounds 6H‐1,3,5‐oxathiazine S,S‐dioxides were found relatively stable, but Lewis acid‐induced thermal reaction afforded the corresponding amides. The plausible pathway to amides from 6H‐1,3,5‐oxathiazine S,S‐dioxides was also discussed in this account. 相似文献
Eichler and Zagier developed a theory of Jacobi forms to understand and extend Maass' work on the Saito‐Kurokawa conjecture. Later Skoruppa introduced skew‐holomorphic Jacobi forms, which play an important role in understanding liftings of modular forms and Jacobi forms. In this paper, we explain a relation between Jacobi forms and skew‐holomorphic Jacobi forms in terms of a group cohomology. More precisely, we introduce an isomorphism from the direct sum of the space of Jacobi cusp forms on and the space of skew‐holomorphic Jacobi cusp forms on with the same half‐integral weight to the Eichler cohomology group of with a coefficient module coming from polynomials. 相似文献
Various selenyl-substituted aryl aldehydes were obtained using dithiothreitol (DTT) as a reducing agent for diselenides in the presence of a base. For electron-deficient haloaryl aldehydes, a weak base, such as K2CO3, performed well. However, for electron-rich haloaryl aldehydes, a stronger base, such as 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU), was required for successful substitution. The selenyl-substitution of heteroaryl substrates were also investigated to obtain satisfactory yields of the desired products. 相似文献
A simple and practical protocol has been developed for the cross-coupling of azoles and aryl iodides under ligand-free copper(I) oxide catalyzed conditions in water. The protocol uses tetrabutylammonium bromide (TBAB) as the phase-transfer catalyst, and water as the solvent, and shows good tolerance towards various functional groups. 相似文献
A split series potential energy function is developed herein such that it can be primarily reduced into generalized forms
of Lennard-Jones, Morse, Buckingham, Linnett, and anything in between them by altering the type-parameters. Unlike the previous
approach whereby the type-parameters act as weighting parameter between the inverse power and the exponential decay terms,
the current approach attaches the type-parameters as indices to the denominator of every summation term. A change of the index
shifts the series summation to mimic a curve that is representative of an inverse power, an exponential index, or a weighted
proportion between the two. Comparison with the Lennard-Jones (12–6) and Exponential-6 (both ξ = 13.772 and ξ = 12) in the case of uncharged non-bonded interactions by using type-parameters (p, q) = (0, 0) and (p, q) = (1, 0) respectively in the model gives very good correlation. Comparison with the Morse potential in the case of bonded
interaction by using (p, q) = (1, 1) gives excellent agreement. All results show that the use of only five expansion terms is sufficient for providing
good agreement with the classical potentials. Better fit to experimental data and ab initio results are expected with the
incorporation of coefficients in every term of the series expansion. The proposed model is useful for modeling the transition
state from solid to fluid and vice versa. 相似文献