The recent development of an automated surface plasmon resonance technology for the measurement of biomolecular interactions (Pharmacia BIAcore) has provided new opportunities for the detection and analysis of protein-protein interactions. In the BIAcore, detection is based on changes in surface plasmon resonance which are monitored optically. Changes in surface plasmon resonance correspond to changes in surface concentration of macromolecules and can be monitored in real time.
We have found that the detection sensitivity obtainable with this technology (ng/ml concentrations of specific ligands are readily detectable for many applications) is complementary “in a bidirectional manner” to micropreparative HPLC. Thus micropreparative HPLC may be used to purify and characterise reagents for the biosensor, whilst the biosensor may be used to define chromatographic parameters such as elution conditions for affinity chromatography or serve as an affinity detector for fractions obtained during chromatographic purification.
Examples of such applications, including the potential of the biosensor to search for and monitor the purification of unknown ligands for which the target molecule has been identified, are shown. In particular, the use of the biosensor to monitor the purification of soluble epidermal growth factor receptor from A431 cell conditioned media is demonstrated. 相似文献
The class
of orthomodular lattices which have only finitely many commutators is investigated. The following theorems are proved:
contains the block-finite orthomodular lattices. Every irreducible element of
is simple. Every element of
is a direct product of a Boolean algebra and finitely many simple orthomodular lattices. The irreducible elements of
which are modular, or are M-symmetric with at least one atom, have height two or less. 相似文献
The heat capacities per unit volume and the densities of aqueous solutions of 2-propanol, neopentanol, tert-amylalcohol, 2-amino-2-methylpropanol, triethylamine and diethylmethylamine were measured, in many cases as a function of temperature, over the whole mole fraction or solubility range. Apparent and partial molal heat capacities, volumes and expansibilities were derived. The concentration dependence of these functions suggest the existence of transitions in some of these systems, in the water-rich region, qualitatively similar to micellization. The large relaxation contribution observed with some of the thermodynamic functions of hydrophobic alcohols and amines suggests a reinforcement of hydrophobic hydration due to strong hydrogen-bonding interactions of the polar groups with water. 相似文献
Abstract 2,5-Diphenyloxazole (POP) is widely used for the determination of radioactivity by scintillation counting. It has been found to be phototoxic to the yeasts Saccharomyces cerevisiae and Candida utilis , to the first instar larvae of the mosquito Aedes aegypti , to the crustaceans Daphnia magna and Artemia salida , as well as to the eggs of Drosophila melanogaster . The related molecule 1,4-bis(5-phenyloxazol-2-yl)benzene (POPOP) is also phototoxic, but to a lesser degree. Both POP and POPOP can sensitize the formation of singlet oxygen. 相似文献
JPC – Journal of Planar Chromatography – Modern TLC - The flavonoid and triterpenoid soyasaponin content of the aerial parts of Desmodium adscendens from four geographical origins of... 相似文献
The considerations which determine the success of controlled-potential coulometric analyses on the submicrogram level are discussed, and it is shown that the ultimate sensitivity of the method is governed by the accuracy with which the requisite background corrections, especially that for the charging quantity of electricity, can be determined. A method for the coulometric determination of zinc based on these considerations is shown to contain a limiting uncertainty of ± 0.2 mμFy, so that as little as 0.07 μg of zinc can be determined within about ± 10%, while quantities of zinc exceeding about 10 μg can be determined with an accuracy and precision of ± 0.1% or better. Possible techniques for the further extension of controlled-potential coulometric analysis into the millimicrogram range are briefly discussed. 相似文献
The demetalation process of 10 multi-iron Wells-Dawson polyoxometalates is studied by cyclic voltammetry and controlled potential coulometry. Eight sandwich-type complexes (alphaalphaalphaalpha-Na(16)[(NaOH(2))(2)(Fe(III))(2)(X(2)W(15)O(56))(2)], alphaalphabetaalpha-Na(14)[(NaOH(2))(Fe(III)OH(2))(Fe(III))(2)(X(2)W(15)O(56))(2)], alphabetabetaalpha-Na(12)[(Fe(III)OH(2))(2)(Fe(III))(2)(X(2)W(15)O(56))(2)], and alphabetabetaalpha-Na(14)[(Mn(II)OH(2))(2)(Fe(III))(2)(X(2)W(15)O(56))(2)] (where X = P(V) or As(V))) and two monomeric complexes (alpha-Na(11)[(P(2)(Fe(III)Cl)(2)(Fe(III)OH(2))W(15)O(59))] and alpha-Na(11)[(As(2)(Fe(III)Cl)(2)Fe(III)OH(2))W(15)O(59))]) were selected for this study. All 10 complexes show Fe(III) waves which are well-separated from the redox activity of the W(VI) centers. At room temperature and under mild conditions, iron release from the complexes is observed upon reduction of the Fe(III) centers. This release is controlled by the ionic strength of the medium, the nature and concentration of the anions present in the supporting electrolyte, and by the pH of the solution. This behavior parallels those described for most siderophores which depend on the same parameters. 相似文献