An Expansion Estimate for Dispersing Planar Billiards with Corner Points

It is known that the dynamics of planar billiards satisfies strong mixing properties (e.g., exponential decay of correlations), provided that some expansion condition on unstable curves is satisfied. This condition has been shown to always hold for smooth dispersing planar billiards, but it needed to be assumed separately in the case of dispersing planar billiards with corner points. We prove that this expansion condition holds for any dispersing planar billiard with corner points, no cusps and bounded horizon.     

Cytokine loaded biopolymers as a novel strategy to study stem cells during wound-healing processes

The biopolymer matrigel loaded with cytokine can be used for the recruitment in vivo of specific cell populations and as a vector for the preparation of cell cultures. Data demonstrate that the injection of the matrigel biopolymer supplemented with interleukin-8 (IL-8) in the leech Hirudo medicinalis can be used to purify cell populations showing the same morphofunctional and molecular mechanisms of specific populations of vertebrate hematopoietic precursor cells involved in tissue repair. These cells spontaneously differentiated into myofibroblasts. This approach highlights how the innovative use of a cytokine-loaded biopolymer for an in vivo cell sorting method, applied to a simple invertebrate model, can be a tool for studying myofibroblast cell biology and its regulation, step by step.     

The use of cation exchange matrix separation coupled with ICP-MS to directly determine platinum group element (PGE) and other trace element emissions from passenger cars equipped with diesel particulate filters (DPF)

Inductively coupled plasma-mass spectrometry coupled with cation exchange matrix separation has been optimised for the direct determination of platinum group element (PGE) and trace element emissions from a diesel engine car. After matrix separation method detection limits of 1.6 ng g(-1) for Pd, 0.4 ng g(-1) for Rh and 4.3 ng g(-1) for Pt were achieved, the method was validated against the certified reference material BCR 723, urban road dust. The test vehicle was fitted with new and aged catalytic converters with and without diesel particulate filters (DPF). Samples were collected after three consecutive New European Driving Cycle (NEDC) of the particulate and "soluble" phases using a home-made sampler optimised for trace element analysis. Emission factors for the PGEs ranged from 0.021 ng km(-1) for Rh to 70.5 ng km(-1) for Pt; when a DPF was fitted, the emission factors for the PGEs actually used in the catalysts dropped by up to 97% (for Pt). Trace element emission factors were found to drop by a maximum of 92% for Ni to a minimum of 18% for Y when a DPF was fitted; a new DPF was also found to cause a reduction of up to 86% in the emission of particulate matter.     

Hybrid Molecules Containing Naphthoquinone and Quinolinedione Scaffolds as Antineoplastic Agents

In recent decades, molecular hybridization has proven to be an efficient tool for obtaining new synthetic molecules to treat different diseases. Based on the core idea of covalently combining at least two pharmacophore fragments present in different drugs and/or bioactive molecules, the new hybrids have shown advantages when compared with the compounds of origin. Hybridization could be successfully applied to anticancer drug discovery, where efforts are underway to develop novel therapeutics which are safer and more effective than those currently in use. Molecules presenting naphthoquinone moieties are involved in redox processes and in other molecular mechanisms affecting cancer cells. Naphthoquinones have been shown to inhibit cancer cell growth and are considered privileged structures and useful templates in the design of hybrids. The present work aims at summarizing the current knowledge on antitumor hybrids built using 1,4- and 1,2-naphthoquinone (present in natural compounds as lawsone, napabucasin, plumbagin, lapachol, α-lapachone, and β -lapachone), and the related quinolone- and isoquinolinedione scaffolds reported in the literature up to 2021. In detail, the design and synthetic approaches adopted to produce the reported compounds are highlighted, the structural fragments considered in hybridization and their biological activities are described, and the structure–activity relationships and the computational analyses applied are underlined.     

Synthesis and Antiproliferative Activity against Cancer Cells of Indole-Aryl-Amide Derivatives

Indoles constitute a large family of heterocyclic compounds widely occurring in nature which are present in a number of bioactive natural and synthetic compounds, including anticancer agents or atypical opioid agonists. As a result, exponential increases in the development of novel methods for the synthesis of indole-containing compounds have been reported in the literature. A series of indole-aryl amide derivatives 1–7 containing tryptamine or an indolylacetic acid nucleus were designed, synthesized, and evaluated as opioid ligands. These new indole derivatives showed negligible to very low affinity for μ- and δ-opioid receptor (OR). On the other hand, compounds 2, 5 and 7 showed Ki values in the low μM range for κ-OR. Since indoles are well known for their anticancer potential, their effect against a panel of tumor cell lines was tested. The target compounds were evaluated for their in vitro cytotoxicity in HT29, HeLa, IGROV-1, MCF7, PC-3, and Jurkat J6 cells. Some of the synthesized compounds showed good activity against the selected tumor cell lines, with the exception of IGROV1. In particular, compound 5 showed a noteworthy selectivity towards HT29 cells, a malignant colonic cell line, without affecting healthy human intestinal cells. Further studies revealed that 5 caused the cell cycle arrest in the G1 phase and promoted apoptosis in HT29 cells.     

Cellulose derivatives as solid electrolyte matrixes

Cellulose derivatives like hydroxyethylcellulose (HEC) and carboxymethylcellulose (CMC) were submitted to the graftization reactions. The samples of HEC were grafted with monoisocyanate of poly(propylene oxide) (MPPO) and different diisocyanates of poly(ethylene oxide) (DPEO) and poly(propylene oxide) (DPPO) in the presence of LiClO₄. CMC was grafted with MPPO. These polyssacharides based samples were characterized by IR, NMR and thermal analysis (DSC and TGA). The samples of grafted polyssacharides derivatives showed glass transition temperatures (Tg) of −40°C for HEC/MPPO, −33°C for HEC/DPEO, −10°C for HEC/DPPO and 24�C for CMC/MPPO. The ionic conductivities of all samples were about 10⁻⁷‐10⁻⁶ S/cm at room temperature. The sample of CMC/MPPO presented conductivity value similar to HEC/DPPO but does not exhibit film‐forming characteristic indicating its use as solid polymer electrolytes only in the pellet form.