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Discrimination of basal cell carcinoma from normal dermal stroma by quantitative multiphoton imaging
Lin SJ Jee SH Kuo CJ Wu RJ Lin WC Chen JS Liao YH Hsu CJ Tsai TF Chen YF Dong CY 《Optics letters》2006,31(18):2756-2758
We performed multiphoton fluorescence (MF) and second-harmonic generation (SHG) imaging on human basal cell carcinoma samples. In the dermis, basal cell carcinomas can be identified by masses of autofluorescent cells with relatively large nuclei and marked peripheral palisading. In the normal dermis, SHG from dermal collagen contributes largely to the multiphoton signal. However, within the cancer stroma, SHG signals diminish and are replaced by autofluorescent signals, indicating that normal collagen structures responsible for SHG have been altered. To better delineate the cancer cells and cancer stroma from the normal dermis, a quantitative MF to SHG index is developed. We demonstrate that this index can be used to differentiate cancer cells and adjacent cancer stroma from the normal dermis. Our work shows that MF and SHG imaging can be an alternative for Mohs' surgery in the real-time guidance of the secure removal of basal cell carcinoma. 相似文献
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Chia‐Wei Chu Xiaomin Zhai Chih Jie Lee Po‐Hao Wang Yubo Duan Din Ping Tsai Baile Zhang Yuan Luo 《Laser \u0026amp; Photonics Reviews》2015,9(4):399-404
Transformation optics, a recent geometrical design strategy of light manipulation with both ray trajectories and optical phase controlled simultaneously, promises an invisibility cloaking device that can render a macroscopic object invisible even to a scientific instrument measuring optical phase. Recent “carpet” cloaks have extended their cloaking capability to broadband frequency ranges and macroscopic scales, but they only demonstrated the recovery of ray trajectories after passing through the cloaks, while whether the optical phase would reveal their existence still remains unverified. In this paper, a phase‐preserved macroscopic visible‐light carpet cloak is demonstrated in a geometrical construction beyond two dimensions. As an extension of previous two‐dimensional (2D) macroscopic carpet cloaks, this almost‐three‐dimensional carpet cloak exhibits three‐dimensional (3D) invisibility for illumination near its center (i.e. with a limited field of view), and its ideal wide‐angle invisibility performance is preserved in multiple 2D planes intersecting in the 3D space. Optical path length is measured with a broadband pulsed‐laser interferometer, which provides unique experimental evidence on the geometrical nature of transformation optics.
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Chao-Qiang Geng Da Huang Lu-Hsing Tsai 《The European Physical Journal C - Particles and Fields》2015,75(11):557
We study the CP-violation effects from two types of neutrino mass matrices with (i) \((M_\nu )_{ee}=0\), and (ii) \((M_\nu )_{ee}=(M_\nu )_{e\mu }=0\), which can be realized by the high-dimensional lepton number violating operators \(\bar{\ell }_R^c\gamma ^\mu L_L (D_\mu \Phi )\Phi ^2\) and \(\bar{\ell }_R^c l_R (D_\mu {\Phi })^2\Phi ^2\), respectively. In (i), the neutrino mass spectrum is in the normal ordering with the lightest neutrino mass within the range \(0.002\,\mathrm{eV}\lesssim m_0\lesssim 0.007\,\mathrm{eV}\). Furthermore, for a given value of \(m_0\), there are two solutions for the two Majorana phases \(\alpha _{21}\) and \(\alpha _{31}\), whereas the Dirac phase \(\delta \) is arbitrary. For (ii), the parameters of \(m_0\), \(\delta \), \(\alpha _{21}\), and \(\alpha _{31}\) can be completely determined. We calculate the CP-violating asymmetries in neutrino–antineutrino oscillations for both mass textures of (i) and (ii), which are closely related to the CP-violating Majorana phases. 相似文献
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Jong-Shenq Guo Hirokazu Ninomiya Je-Chiang Tsai 《Physica D: Nonlinear Phenomena》2010,239(3-4):230-239
Recent experimental studies of photosensitive Belousov–Zhabotinskii reaction have revealed the existence of propagating wave segments. The propagating wave segments are unstable, but can be stabilized by using a feedback control to continually adjust the excitability of the medium. Experimental studies also indicate that the locus of the size of a stabilized wave segment as a function of the excitability of the medium gives the excitability boundary for the existence of 2D wave patterns with free ends in excitable media. To study the properties of this boundary curve, we use the wave front interaction model proposed by Zykov and Showalter. This is equivalent to study a first order system of three ordinary differential equations which includes a singular nonlinearity. Using two different reduced first order systems of two ordinary differential equations, we first show the existence of wave segments for any given propagating velocity. Then the wave profiles can be classified into two types, namely, convex and non-convex types. More precisely, when the normalized propagating velocity is small, we show that the wave profile is of convex type, while the wave profile is of non-convex type when the normalized velocity is close to 1. 相似文献
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Liu SH Lin YH Huang LJ Luo SW Tsai WL Chiang SY Fung HS 《Journal of synchrotron radiation》2010,17(6):761-768
A synchrotron‐radiation‐based circular‐dichroism end‐station has been implemented at beamline BL04B at the National Synchrotron Radiation Research Center (NSRRC) in Taiwan for biological research. The design and performance of this compact end‐station for measuring circular‐dichroism spectra in the vacuum‐ultraviolet region are described. The linearly polarized light from the beamline is converted to modulated circularly polarized light with a LiF photoelastic modulator to provide a usable wavelength region of 130–330 nm. The light spot at the sample position is 5 mm × 5 mm at a slit width of 300 µm and provides a flux greater than 1 × 1011 photons s?1 (0.1% bandwidth)?1. A vacuum‐compatible cell made of two CaF2 windows has a variable path length from 1.3 µm to 1 mm and a temperature range of 253–363 K. Measured CD spectra of (1S)‐(+)‐10‐camphorsulfonic acid and proteins demonstrated the ability of this system to extend the wavelength down to 172 nm in aqueous solution and 153 nm in hexafluoro‐2‐propanol. 相似文献
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Polyelectrolyte multilayer (PEM) films offer a method to functionalize substrates with specific properties that enable the films to be used for a variety of purposes. Desirable qualities of PEM films can include mechanical strengths, ease of preparations, flexibility, and their abilities to have their properties tailored to suit a particular process. We present a simple method to fabricate a class of PEM films that incorporate cubic silsesquioxane nanoparticles (CSSQ). Through a spin self-assembly (SSA) process, a hybrid multilayered film with two-components, namely, poly(styrene sulfonate) (PSS) and octaammonium cubic silsesquioxane (CSSQ) nanoparticle have been fabricated. The formation of this multilayer film is further verified by ellipsometry, contact angle studies, and atomic force microscopy (AFM). The water contact angle and ellipsometric measurements exhibit that the (PSS/OA-CSSQ) films are deposited onto the substrate. The surface topography of the deposited bilayers of PSS/OA-CSSQ film appears to be uniformly distributed with extremely small granules but the film uniformity of the granular surface is diminished and clusters of granules are observed at above 5 bilayers due to the aggregation of the OA-CSSQ nanoparticles. 相似文献