In this paper we discuss a novel application of holographic interferometry in the simultaneous quantitative visualization
of high-speed, oscillatory flow and temperature fields in complex flow geometries. We consider cases of (i) self-sustained
oscillatory flows with main flow imposed in grooved and communicating channels as well as (ii) oscillating thermofluid processes
with zero mean velocity in a thermoacoustic refrigerator model. Examples showing unsteady temperature distributions obtained
by real-time holographic interferometry combined with high-speed cinematography illustrate the possibilities of the approach
introduced in the paper. Our study shows that temperature distributions accurately mirror flow structures in certain types
of complex, unsteady flows, thus allowing, apart from the measurement of temperature profiles and heat transfer, also the
measurement of oscillatory amplitudes, frequencies, wavelengths as well as the speed of propagation of traveling waves by
applying digital image processing techniques. In the grooved and communicating channels it is possible to visualize the structure
of the Tollmien–Schlichting waves through isotherms by using the infinite fringe field alignment of holographic interferometry.
In the thermoacoustic refrigerator model, small amplitude temperature oscillations generated by the acoustic standing wave
are visualized and measured. Image processing as well as data reduction procedures used in the analysis of these flow fields
are discussed in the paper. Experimental data obtained by applying the techniques introduced in the paper show good agreement
with theory and results of numerical simulations. Our study suggests that using temperature as tracer offers numerous advantages
in the study of certain types of complex, unsteady flows.
Received: 7 January 1997/Accepted: 18 September 1997 相似文献
A box-tree is a bounding-volume hierarchy that uses axis-aligned boxes as bounding volumes. We describe a new algorithm to construct a box-tree for objects in a 3D scene, and we analyze its worst-case query time for approximate range queries. If the input scene has certain characteristics that we derived from our application—collision detection in industrial installations—then the query times are polylogarithmic, not only for searching with boxes but also for range searching with other constant-complexity ranges. 相似文献
6-Fluoro-8-quinolinol was prepared from 2-amino-5-fluorophenol by a Skraup synthesis. No synergism was observed between 5-fluoro- and 6-fluoro-8-quinolinols or between 6-fluoro- and 7-fluoro-8-quinolinols against any of the six fungi in our test system (Aspergillus niger, A. oryzae, Myrothecium verrucaria, Trichoderma viride, Mucor cirinelloides, and Trichophyton mentagrophytes) in Sabouraud dextrose broth. Unlike the fluoro-8-quinolinols, the 8-quinolinols comparably substituted with chlorine or bromine did form synergistic mixtures. This is attributed to steric factors. 相似文献
Ionic fluorophores are powerful tools for the study of environmental science and bio‐imaging. However, traditional ionic dyes usually require long synthetic steps and suffer from a quenching effect caused by aggregation. A water‐soluble ionic aggregation‐induced emission luminogen called DBTA is presented, which is readily accessed by a one‐step reaction. The switchable emission manipulated by hydrogen bonding provided solid evidence for the restriction of intramolecular motions as the mechanism of aggregation‐induced emission. DBTA can not only differentiate solvents with different H‐bond donor acidities but also capable of wash‐free imaging in living HeLa cells and fish larva. 相似文献
A single high‐affinity fatty acid binding site in the important human transport protein serum albumin (HSA) is identified and characterized using an NBD (7‐nitrobenz‐2‐oxa‐1,3‐diazol‐4‐yl)‐C12 fatty acid. This ligand exhibits a 1:1 binding stoichiometry in its HSA complex with high site‐specificity. The complex dissociation constant is determined by titration experiments as well as radioactive equilibrium dialysis. Competition experiments with the known HSA‐binding drugs warfarin and ibuprofen confirm the new binding site to be different from Sudlow‐sites I and II. These binding studies are extended to other albumin binders and fatty acid derivatives. Furthermore an X‐ray crystal structure allows locating the binding site in HSA subdomain IIA. The knowledge about this novel HSA site will be important for drug depot development and for understanding drug‐protein interaction, which are important prerequisites for modulation of drug pharmacokinetics. 相似文献
Treatment of CpRuH(PP) (PP=dppm, dppe) with TlPF6 produced [CpRu(H)(Tl)(PP)]PF6. X‐ray diffraction and computational studies suggest that the complexes contain a Ru?H?Tl 3c–2e bond and can be viewed as the first σ‐complexes of period 6 main‐group hydrides [CpRu{η2‐(H?Tl)}(PP)]PF6 or [Tl{η2‐H?RuCp(PP)}]PF6. The complexes can be stored as a solid at room temperature for days without appreciable decomposition, but are unstable in solution and evolved to the trimetallic complexes [{CpRu(PP)}2(μ‐Tl)]PF6. 相似文献
New estrane salicyloyloxy or D-homo derivatives were synthesized under microwave (MW) or conventional heating from estrane precursors and methyl salicylate. The MW technique provides advantages regarding product yield and reaction time, and represents a more environmentally friendly approach than conventional heating. Considering the biomedical potential of estrane compounds, we evaluated the antioxidant activity and cytotoxicity of synthesized estrane derivatives in a series of in vitro tests, as well as their 3β-hydroxysteroid dehydrogenase/Δ5 → Δ4 isomerase (3βHSD) and 17β-hydroxysteroid dehydrogenase types 1, 2 and 3 (17βHSD1, 17βHSD2 and 17βHSD3) inhibition potentials. In DPPH tests, 3-methoxyestra-1,3,5(10)-trien-17β-yl salicylate displayed antioxidant potential, while all compounds exhibited OH radical neutralization activity. 3-Oxoestr-4-en-17β-yl salicylate showed strong cytotoxicity against MDA-MB-231 breast cancer cells, while 17-oxoestra-1,3,5(10)-trien-3-yl salicylate, estra-1,3,5(10)-triene-3,17β-diyl 3-benzoate 17-salicylate and 3-benzyloxy-17-salicyloyloxy-16,17-secoestra-1,3,5(10)-triene-16-nitrile showed the strongest inhibition of PC-3 prostate cancer cell growth. 3-Hydroxyestra-1,3,5(10)-trien-17β-yl salicylate was the best inhibitor of 17βHSD2, suggesting potential use in treating pathological conditions associated with estrogen depletion. For 3-methoxyestra-1,3,5(10)-trien-17β-yl salicylate and 3-oxoestr-4-en-17β-yl salicylate, X-ray crystal structure analysis and molecular energy optimization were performed to define their conformations and energy minima. Very good overlap in the region of the steroidal nucleus was observed for the molecular structures of each analyzed molecule in the crystalline state and after energy optimization, while conformer analysis indicates conformational flexibility in the form of rotation around the C17···O2 bond. Structural geometry analysis for these compounds shows that the region of ring A in steroids, and especially the C3 atom functional group, is important structural features concerning antiproliferative activity against MDA-MB-231 cells.
Mono‐ADP‐ribosylation is a dynamic posttranslational modification (PTM) with important roles in signaling. Mammalian proteins that recognize or hydrolyze mono‐ADP‐ribosylated proteins have been described. We report the synthesis of ADP‐ribosylated peptides from the proteins histone H2B, RhoA and, HNP‐1. An innovative procedure was applied that makes use of pre‐phosphorylated amino acid building blocks. Binding assays revealed that the macrodomains of human MacroD2 and TARG1 exhibit distinct specificities for the different ADP‐ribosylated peptides, thus showing that the sequence surrounding ADP‐ribosylated residues affects the substrate selectivity of macrodomains. 相似文献