Whole cell matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and in situ structure analysis of streptocidins, a family of tyrocidine-like cyclic peptides

Streptocidins, a family of tyrocidine-like cyclic decapeptides, are an ideal demonstration object for the detection and in situ structure analysis of natural compounds directly in microbial cells using whole cell matrix-assisted laser desorption/ionization time-of-flight-mass spectrometry (MALDI-TOFMS), an emerging technique that can be used for rapid sensitive metabolic profiling of microorganisms. Five main members of the streptocidin family (A-E) were detected in Brevibacillus cells picked from agar plates and identified by in situ structure analysis with post-source decay MALDI-TOFMS. This efficient modern method allows the precise detection of metabolites within minutes without the need to isolate and purify the target compounds. The generated mass spectra are of similar quality to those obtained for the purified peptides. In addition, surface extracts were prepared by treating Brevibacillus cells with 70% acetonitrile in the presence of 0.1% trifluoroacetic acid and fractionated by high-resolution reversed-phase high-performance liquid chromatography (HPLC). In this way ten minor streptocidins were detected demonstrating the full biosynthetic variety of streptocidin production on the cellular level. The streptocidins differ from the well-known tyrocidines essentially in position 3 of the decapeptide chain by replacement of the aromatic amino acid (F/W) found in tyrocidines by L-leucine or L-valine.     

Long-range pair correlations in dilute gases

It is shown that there exist nonequilibrium pair correlations which are not described by the Boltzmann transport equation but which persist even in a dilute gas.     

Isolation and total synthesis of (E)-6, 10-dimethyl-9-methylidene-undec-5-en-2-one,a constituent of Costus root oil

(E)-6, 10-Dimethyl-9-methylidene-undec-5-en-2-one ( 1 ) has been isolated from Costus root oil. A total synthesis of this compound is reported.     

Zwitterionic 1-phosphonioalkyl dithiophosphinates

The title compounds (and, in part, their seleno analogs) result from the oxidation of an ylidylphosphine and also from the addition of ylides to a perthiophosphonic anhydride. They can be deprotonated and alkylated to give anions or cations, respectively. The reaction with phenacyl bromide opens a way to 1,3,2-oxathiaphosphole sulfides. © 1998 John Wiley & Sons, Inc. Heteroatom Chem 9:433–437, 1998     

Scalable ion trap quantum computing without moving ions

A hybrid quantum computing scheme is studied where the hybrid qubit is made of an ion trap qubit serving as the information storage and a solid-state charge qubit serving as the quantum processor, connected by a superconducting cavity. In this paper, we extend our previous work [CITE] and study the decoherence, coupling and scalability of the hybrid system. We present our calculations of the decoherence of the coupled ion-charge system due to the charge fluctuations in the solid-state system and the dissipation of the superconducting cavity under laser radiation. A gate scheme that exploits rapid state flips of the charge qubit to reduce decoherence by the charge noise is designed. We also study a superconducting switch that is inserted between the cavity and the charge qubit and provides tunable coupling between the qubits. The scalability of the hybrid scheme is discussed together with several potential experimental obstacles in realizing this scheme.     

A measuring system for the fast simultaneous isotope ratio and elemental analysis of carbon, hydrogen, nitrogen and sulfur in food commodities and other biological material

The isotope ratio of each of the light elements preserves individual information on the origin and history of organic natural compounds. Therefore, a multi-element isotope ratio analysis is the most efficient means for the origin and authenticity assignment of food, and also for the solution of various problems in ecology, archaeology and criminology. Due to the extraordinary relative abundances of the elements hydrogen, carbon, nitrogen and sulfur in some biological material and to the need for individual sample preparations for H and S, their isotope ratio determination currently requires at least three independent procedures and approximately 1 h of work. We present here a system for the integrated elemental and isotope ratio analysis of all four elements in one sample within 20 min. The system consists of an elemental analyser coupled to an isotope ratio mass spectrometer with an inlet system for four reference gases (N(2), CO(2), H(2) and SO(2)). The combustion gases are separated by reversible adsorption and determined by a thermoconductivity detector; H(2)O is reduced to H(2). The analyser is able to combust samples with up to 100 mg of organic material, sufficient to analyse samples with even unusual elemental ratios, in one run. A comparison of the isotope ratios of samples of water, fruit juices, cheese and ethanol from wine, analysed by the four-element analyser and by classical methods and systems, respectively, yielded excellent agreements. The sensitivity of the device for the isotope ratio measurement of C and N corresponds to that of other systems. It is less by a factor of four for H and by a factor of two for S, and the error ranges are identical to those of other systems.