异硫氰酸荧光素标记抗体的固体基质室温磷光性质研究

研究了异硫氰酸荧光素（FITC）标记的羊抗人抗体（GAHAb-FITC)和兔抗羊抗体9RAGAb-FITC)，及其以三种免疫方式与人免疫球蛋白（IgG)反应所得免疫复合物在多种固体薄膜基质上发射室温磷光（RTP）的适宜条件及其光谱，强度和寿命等性质。研究发现，标记抗体及其免疫复合物保留了FITC优良的固体基质室温磷光性质，λ m0643em^(max)=525/650nm，其RTP强度与其浓度线性相关，并有很高的灵敏度，更为重要的是，免疫反应及其RTP检测能结合于同一基质，方便地相继完成，在聚酰胺膜（PM）上，以Pb(Ac)2为重原子微扰剂，稀释比为1：10（ψ）的GAHAb-FITC,RAGAb-FITC及其与人IgG形成的免疫复合物均能发射较强制 RTP信号并有较长的RTP寿命，本结果为建立相应的固体基质室温磷光免疫分析（SS-RTP-IA）新方法奠定了相应的实验基础。     

940nm波长高功率线阵二极管激光器封装研究

 对940nm波长高功率线阵二极管激光器的封装结构进行了重新设计，并在此基础上开展了芯片的封装实验。封装出的线阵二极管激光器，在连续工作时输出激光功率达40W，在准连续工作时（占空比0.5%），输出峰值功率可达100W。     

AC conductivity of porous silicon: A fractal and surface transport mechanism?

Summary In this paper we report on the frequency dependence of the AC conductivity of porous silicon in the range 10 Hz-100 kHz. Two types of testing devices have been fabricated on three different series of samples formed electrochemically using as a starting materialptype,n ⁻-type andn ⁺-type silicon substrates. For frequencies less than 20-40 kHz the conductivity is found to follow a sublinear frequency dependence. This behaviour is typical of a carrier transport mechanism determined by an anomalous diffusion process. At higher frequencies we find that surface states influence the transport mechanism. This suggests a double-channel transport mechanism: one related to porous-silicon “volume” properties and the other more connected to the “surface” itself. Paper presented at the III INSEL (Incontro Nazionale sul Silicio Emettiore di Luce) Torino, 12–13 October 1995.     

k—Very Ample Line Bundles on Del Pezzo Surfaces

A k-very ample line bundle L on a Del Pezzo Surface is numerically characterized, improving the results of Biancofiore— Ceresa in [7].     

Maximum spin aligned states in 98 47Ag51

New excited states have been observed in the neutron deficient isotope ⁹⁸Ag following the ⁵⁸Ni+⁵⁰Cr →¹⁰⁸Te^* heavy–ion reaction. One of these states may be interpreted as a maximum spin aligned state of the πg_9/2 ⁻³νg_7/2 configuration. Other possible interpretations within the nuclear shell model are discussed as well. Received: 24 October 1997     

Three-phase compressible flow in porous media: Total Differential Compatible interpolation of relative permeabilities

We describe the construction of Total Differential (TD) three-phase data for the implementation of the exact global pressure formulation for the modeling of three-phase compressible flow in porous media. This global formulation is preferred since it reduces the coupling between the pressure and saturation equations, compared to phase or weighted formulations. It simplifies the numerical analysis of the problem and boosts its computational efficiency. However, this global pressure approach exists only for three-phase data (relative permeabilities, capillary pressures) which satisfy a TD condition. Such TD three-phase data are determined by the choice of a global capillary pressure function and a global mobility function, which take both saturations and global pressure level as argument. Boundary conditions for global capillary pressure and global mobility are given such that the corresponding three-phase data are consistent with a given set of three two-phase data. The numerical construction of global capillary pressure and global mobility functions by C¹${\text{C}}^1$ and C⁰${\text{C}}^0$ finite element is then performed using bi-Laplacian and Laplacian interpolation. Examples of the corresponding TD three-phase data are given for a compressible and an incompressible case.     

Towards Nanoscale Biomedical Devices in Medicine: Biofunctional and Spectroscopic Characterization of Superparamagnetic Nanoparticles

Medical interest in nanotechnology originates from a belief that nanoscale therapeutic devices can be constructed and directed towards its target inside the human body. Such nanodevices can be engineered by coupling superparamagnetic nanoparticle to biomedically active proteins. We hereby report the immobilization of a PhEst, a S-formylglutathione hydrolase from the psychrophilic P. haloplanktis TAC125 onto the gold coated surface of modified superparamagnetic core-shell nanoparticles (Fe₃O₄@Au). The synthesis of the nanoparticles is also reported. S-formylglutathione hydrolases constitute a family of ubiquitous enzymes which play a key role in formaldehyde detoxification both in prokaryotes and eukaryotes. PhEst was originally annotated as a putative feruloyl esterase, an enzyme that releases ferulic acid (an antioxidant reactive towards free radicals such as reactive oxygen species) from polysaccharides esters. Dynamic light scattering, scanning electron microscopy with energy dispersive X-ray spectroscopy, UV–visible absorption spectroscopy, fluorescence spectroscopy, magnetic separation technique and enzyme catalytic assay confirmed the chemical composition of the gold covered superparamagnetic nanoparticles, the binding and activity of the enzyme onto the nanoparticles. Activity data in U/ml confirmed that the immobilized enzyme is approximately 2 times more active than the free enzyme in solution. Such particles can be directed with external magnetic fields for bio-separation and focused towards a medical target for therapeutical as well as bio-sensor applications.     

Maximally aligned states in 99Ag

Excited states of ⁹⁹Ag were populated via the ⁵⁰Cr + ⁵⁸Ni (261 MeV) reaction using the NORDBALL detector array equipped with charged-particle and neutron detector systems for reaction channel separation. On the basis of the measured γγ-coincidence relations and angular distribution ratios a significantly extended level scheme has been constructed up to E _x ∼ 7.8 MeV and I = 35/2. The experimental results were described within the framework of the shell model. Candidates for states fully aligned in the πg _9/2 ^-3ν(d _5/2, g _7/2)² valence configuration space were found at 4109 and 6265 keV. Received: 18 June 2002 / Accepted: 11 October 2002 / Published online: 4 February 2003 RID="a" ID="a"e-mail: sohler@atomki.hu Communicated by J. ?yst?     

Spectroscopy of neutron deficient 65Ge

Excited states of ⁶⁵Ge were populated via the ¹²C + ⁵⁸Ni (261 MeV) reaction using the NORDBALL detector array equipped with charged-particle and neutron detector systems for reaction channel separation. On the basis of γγ-coincidence relations and angular distribution ratios a significantly extended level scheme was constructed up to E _x = 9 MeV and J ^π = (33/2^?). The low-energy states of the nucleus are discussed in the framework of the interacting boson-fermion model.