Application of elemental bioimaging using laser ablation ICP-MS in forest pathology: distribution of elements in the bark of Picea sitchensis following wounding

Element distribution in the bark of two 20-year-old clones of Picea sitchensis following wounding was studied using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). Bark was sampled at 0, 3, and 43 days after wounding and analysed using a focused Nd:YAG laser (266 nm). Intensities of ¹³ C, ²⁵Mg, ²⁷Al, ³¹P, ³²S, ³⁹K, ⁴⁸Ca, ⁵⁵Mn, ⁵⁷Fe, ⁶³Cu and ⁶⁴Zn were measured by ICP-MS to study elemental distribution across the bark samples during the wound repair process. A clear accumulation of Mg, P and K at the boundary zone between the lesion and healthy tissue was detected in the wounded samples and was more distinctive at 43 than at 3 days after treatment. This zone of accumulation mapped onto the position of formation of the ligno-suberised boundary zone and differentiation of the wound periderm. These accumulations suggest major roles for Mg, P and K in the non-specific response of Sitka spruce both to wounding, possibly as co-factors to enzymes and energy utilisation. The LA-ICP-MS method developed in this work proved useful to study spatial element distribution across bark samples and has great potential for applications in other areas of plant pathology research.     

Phase-Contrast Mammography at the SYRMEP Beamline of Elettra

At the SYnchrotron Radiation for MEdical Physics (SYRMEP) beamline of Elettra, a widespread research activity in bio-medical imaging has been developed since 1997 [1 Arfelli, F. 1998. Low-dose phase contrast X-ray medical imaging. Phys. Med. Biol, 43: 2845 [Google Scholar]]. The core program carried out by the SYRMEP research team concerns the use of synchrotron radiation (SR) for mammography in the effort of improving image diagnostic quality innovating the imaging technique and the detection system.     

A class of bivariate exponential distributions

We introduce a class of absolutely continuous bivariate exponential distributions, generated from quadratic forms of standard multivariate normal variates.This class is quite flexible and tractable, since it is regulated by two parameters only, derived from the matrices of the quadratic forms: the correlation and the correlation of the squares of marginal components. A simple representation of the whole class is given in terms of 4-dimensional matrices. Integral forms allow evaluating the distribution function and the density function in most of the cases.The class is introduced as a subclass of bivariate distributions with chi-square marginals; bounds for the dimension of the generating normal variable are underlined in the general case.Finally, we sketch the extension to the multivariate case.     

Oxygen-17 NMR chemical shifts of esters

The ¹⁷O NMR spectra of some esters are discussed and are correlated with ¹³C NMR chemical shift and IR carbonyl streching band data.     

Terpene biosynthesis in the nudibranch Doriopsilla areolata

Biogenesis of the enantiomeric sesquiterpenes 1 and 5 of the marine nudibranch Doriopsilla areolata was investigated by feeding of [1-(13)C]glucose, [1,2-(13)C(2)]glucose, and [1,2-(13)C(2)]acetate. Evidence is presented that supports de novo origin of both compounds via mevalonic acid.     

Uniform convergence of derivatives of extended Lagrange interpolation

Summary The authors construct some extended interpolation formulae to approximate the derivatives of a function in uniform norm. They prove theorems on uniform convergence and give estimates of pointwise type and of simultaneous approximation.This material is based upon work supported by the Italian Research Council (first and second authors), and by the Ministero dell'Università e della Ricerca Scientifica e Tecnologica (second and third author).     

New C16 fatty-acid-based oxylipin pathway in the marine diatom Thalassiosira rotula

An unprecedented series of C16 oxylipins (1-8) has been characterized from the marine diatom Thalassiosira rotula. Absolute stereochemistry of the major alcohols 1 and 3 was determined to be 9S by spectroscopic and chemical methods. All the described products are formally derived by unprecedented enzymatic oxidation of C16 fatty acids. Conversion of hexadeca-6,9,12-trienoic acid (C16:3 omega-4) into 3 unequivocally established the occurrence of (at least) a specific 9S-oxygenase activity. To the best of our knowledge, the present data reveal for the first time the existence of an organic network of oxygenase-mediated transformations that require C16 fatty acids as substrates in living cells.