树脂吸附法分离纯化桑叶总黄酮(Ⅰ)H103树脂对桑叶水提液中总黄酮的吸附性能

采用大孔吸附树脂法从桑叶水提液中分离黄酮类化合物。通过比较10种大孔吸附树脂对桑叶水提液中总黄酮的吸附特性及机理,发现H103树脂对桑叶总黄酮吸附量大、洗脱容易、吸附速度快,是一种良好的桑叶总黄酮吸附剂。实验表明,H103树脂吸附桑叶黄酮的适宜上样浓度为6.05mg/mL,吸附动力学符合Bangham模型,吸附过程符合内扩散模型。     

Purification and Characterization of the Catalytic Domain of Protein Tyrosine Phosphatase SHP-1 and the Preparation of Anti-ΔSHP-1 Antibodies

This study is focused on the expression of an SH2 domain-truncated form of protein tyrosine phosphatase SHP-1（designated ΔSHP-1） and the preparation of its polyclonal antibodies. A cDNA fragment encoding ΔSHP-1 was amplified by PCR and then cloned into the pT7 expression vector. The recombinant pT7-ΔSHP-1 plasmid was used to transform Rosetta（DE3） E. coli cells. ΔSHP-1 was distributed in the exclusion body of E. coli cell extracts and was purified through a two-column chromatographic procedure. The purified enzyme exhibited an expected molecular weight on SDS-gels and HPLC gel filtration columns. It possesses robust tyrosine phosphatase activity and shows typical enzymatic characteristics of classic tyrosine phosphatases. To generate polyclonal anti-ΔSHP-1 antibodies, purified recombinant ΔSHP-1 was used to immunize a rabbit. The resultant anti-serum was subjected to purification on ΔSHP-1 antigen affinity chromatography. The purified polyclonal antibody displayed a high sensitivity and specificity toward ΔSHP-1. This study thus provides the essential materials for further investigating the biological function and pathological implication of SHP-1 and screening the inhibitors and activators of the enzyme for therapeutic drug development.     

Synthesis, Characterization, and Properties of Supported Tungstozincate Bridged by Co(Ⅱ) Complex Fragment

[CoII(phen)3]2[{(ZnW12O40)CoII(phen)2(H2O)}2CoII(trien)2(NaH2O)2]·3H2O was synthesized via hydrothermal technique and characterized with elemental analyses, IR spectroscopy, TGA-DTA, and variable temperature magnetic susceptibility. The compound crystallized in the monoclinic system with the space group P21/n, a=1.8210 nm, b=2.3592 nm, c=2.2932 nm, β=110.31o, V=9.239 nm3, Z=2, R1=0.0827. The compound consists of two coordination cations, three lattice water molecules, and a macroanion [{(ZnW12O40)Co(phen)2(...     

Differential pulse voltammetric studies on the effects of Al（Ⅲ） on the lactate dehydrogenase activity

In this paper, differential pulse voltammetry (DPV) was applied to study the effects of aluminum Al(Ⅲ) on the lactate dehydrogenase (LDH) activity. Michaelis–Menten constant (KmN ADH) and maximum velocity (vmax) in the enzyme promoting catalytic reaction of "pyruvate(Pyr) NADH H LDDHlactate NAD " under different conditions by monitoring DPV reduction current of NAD were reported.     

合肥光源直流流强检测器系统的磁屏蔽

分析和测试了合肥光源杂散场对直流流强检测器系统的影响。给出了合肥光源ＤＣＣＴ系统的磁屏蔽的计算、设计和测量结果。屏蔽后,由杂散场引起的ＤＣＣＴ零漂由１ｍＡ降至１０μＡ以下。     

利用FEL康普顿背散射实现对NSRL辐射波段的扩展

 针对NSRL同步辐射光源不能开展硬X射线领域实验研究的缺点,提出了利用FEL康普顿背散射产生高亮度极化的硬X射线,并利用FEL波长可调的特点来连续调节硬X射线的波长。同时还针对NSRL光源的参数,提出了一个初步可行的远红外FEL参数和背散射工作方式,并给出了理论结果。     

反向交叉相位调制对NOLM开关特性影响

提出了NOLM中反向信号脉冲与控制脉冲耦合方程,研究了正、反向信号脉冲在传输过程中共同作用的非线性相移,分析了高斯脉冲和NRZ码在此情况下的开关特性,得到了有反向交叉相位调制时的第一最佳解复用长度、开关窗口和输出信号波形等对实际应用有价值的数据和波形.     

合肥国家同步辐射光源软X射线束空间相干性实验研究

本文从实验角度对合肥国家同步辐射实验室软X射线束的空间相干性进行了研究.利用放置在物面上的高分辨正性光刻胶,记录了中心波长为3.2nm的波带片和针孔直径为30μm组合的光场分布(光斑),获得相应条件下的爱里斑及空间相干直径实验数据;完成了一个双缝干涉实验.所得的这些结果与理论计算相一致.     

四元数方法及其应用

本文全面地评述了四元数方法及其在现代科学技术中的应用,指出四元数具有其他定位参数和变换算子的综合优点和综合功能,不仅在刚体动力学中有广泛的用途,而且在一般多体系统的力学分析和控制中也有极其广阔的应用前景。      

运动稳定性的研究进展和趋势

本文概述运动稳定性学科在力学系统、控制系统、大系统、冲击系统、不确定系统以及一般理论等方面的研究进展和趋势。