The compact integration of a cascaded HCR circuit for highly reliable cancer cell discrimination

The accurate identification of multiple biomarkers involved in disease plays a vital role in effectively distinguishing cancer cells from normal cells, facilitating reliable cancer diagnosis. Motivated by this knowledge, we have engineered a compact and clamped cascaded DNA circuit for specifically discriminating cancer cells from normal cells via the amplified multi-microRNA imaging strategy. The proposed DNA circuit combines the traditional cascaded DNA circuit with multiply localized responsive character through the elaboration of two super-hairpin reactants, thus concurrently streamlining the circuit components and realizing localization-intensified cascaded signal amplification. In parallel, the multiple microRNA-stimulated sequential activations of the compact circuit, combined with a handy logic operation, significantly elevated the cell-discriminating reliability. Applications of the present DNA circuit in vitro and in cellular imaging experiments were executed with expected results, therefore illustrating that our DNA circuit is useful for precise cell discrimination and further clinical diagnosis.

A compact and clamped CHA-control-HCR (CCH) circuitry system, specifically for amplified multi-microRNA imaging, is developed to precisely distinguish cancer cells from normal cells.     

Enzyme-free amplified detection of DNA by an autonomous ligation DNAzyme machinery

The Zn(2+)-dependent ligation DNAzyme is implemented as a biocatalyst for the amplified detection of a target DNA by the autonomous replication of a nucleic acid reporter unit that is generated by the catalyzed ligation process. The reporter units enhance the formation of active DNAzyme units, thus leading to the isothermal autocatalytic formation of the reporter elements. The system was further developed and applied for the amplified detection of Tay-Sachs genetic disorder mutant, with a detection limit of 1.0 × 10(-11) M. Besides providing a versatile paradigm for the amplified detection of DNA, the system reveals a new, enzyme-free, isothermal, autocatalytic mechanism that introduces means for effective programmed synthesis.     

变螺旋角方法离心风机蜗壳型线数值优化

基于变螺旋角蜗壳型线设计方法,通过大涡模拟(LES)计算风机内部的非定常流场,利用二次回归正交试验设计法,对某离心风机的蜗壳型线进行数值优化以降低风机的A声级。优化时以蜗壳型线起始和终了位置的螺旋角为设计变量,以非定常流场计算得出的时均效率和A声级为目标函数,通过优化得到使风机气动噪声最低的最佳螺旋角分布,进而得到最佳蜗壳型线。利用数值模拟对优化结果进行了验证,验证结果表明:优化之后,时均效率降低了0.07%,A声级降低了4.04dB,时均效率和A声级相对于原风机分别降低了0.09%和4.51%;本次二次回归正交试验优化具有较高的预测精度,与CFD数值验证得出的时均效率和A声级的相对误差分别为0.01%和0.53%。     

强酸性阳离子交换树脂催化丙烯齐聚

筛选了可用于丙烯齐聚的阳离子交换树脂催化剂,研究了树脂性质与催化性能间的关系,并考察了自制LC001树脂催化丙烯齐聚的工艺条件.结果发现,采用大孔、高交换量的LC001树脂在150℃、4.0MPa、液时空速为1.5h-1条件下反应,丙烯单程转化率达到64.7％,C9和C12烯烃的选择性能达到58％.催化剂性能稳定,催化活性高,工艺流程简单,有很好的工业应用前景.