Targeting of cancer cells using click-functionalized polymer capsules

Targeted delivery of drugs to specific cells allows a high therapeutic dose to be delivered to the target site with minimal harmful side effects. Combining targeting molecules with nanoengineered drug carriers, such as polymer capsules, micelles and polymersomes, has significant potential to improve the therapeutic delivery and index of a range of drugs. We present a general approach for functionalization of low-fouling, nanoengineered polymer capsules with antibodies using click chemistry. We demonstrate that antibody (Ab)-functionalized capsules specifically bind to colorectal cancer cells even when the target cells constitute less than 0.1% of the total cell population. This precise targeting offers promise for drug delivery applications.     

The Ternary System Nickel/Silicon/Titanium Revisited

The constitution of the ternary system Ni/Si/Ti is investigated over the entire composition range using X‐ray diffraction (XRD), energy dispersive X‐ray spectroscopy (EDS), differential thermal analysis (DTA), and metallography. The solid state phase equilibria are determined for 900 °C. Eight ternary phases are found to be stable. The crystal structures for the phases τ₁NiSiTi, τ₂Ni₄Si₇Ti₄, τ₃Ni₄₀Si₃₁Ti₁₃, τ₄Ni₁₇Si₇Ti₆, and τ₅Ni₃SiTi₂ are corroborated. For the remaining phases the compositions are determined as Ni₆Si₄₁Ti₅₃ (τ₆), Ni₁₆Si₄₂Ti₄₂(τ₇), and Ni₁₂Si₄₅Ti₄₃ (τ₈). The reaction scheme linking the solid state equilibria with the liquidus surface is amended to account for these newly observed phases. The discrepancies between previous experimental conclusions and modeling results are addressed. The liquidus surface is dominated by the primary crystallisation field of τ₁NiSiTi, the only congruently melting phase.     

Crystal structure investigations of ZrAsxSey (x>y, x+y≤2) by single crystal neutron diffraction at 300 K, 25 K and 2.3 K

Large single crystals of ZrAs_xSe_y (x>y, x+y≤2, PbFCl type of structure, space group P4/nmm) were grown by Chemical Transport. Structural details were studied by single crystal neutron diffraction techniques at various temperatures. One single crystal specimen with chemical composition ZrAs_1.595(3)Se_0.393(1) was studied at ambient temperature (R1=5.10 %, wR2=13.18 %), and a second crystal with composition ZrAs_1.420(3)Se_0.560(1) was investigated at 25 K (R1=2.70%, wR2=5.70 %) and 2.3 K (R1=2.30 %, wR2=4.70 %), respectively. The chemical compositions of the crystals under investigation were determined by wavelength dispersive X-ray spectroscopy. The quantification of trace elements was carried out by Laser Ablation-Inductively Coupled Plasma-Mass Spectrometry. According to the crystal structure refinements the crystallographic 2a site is occupied by As, together with a significant amount of vacancies. One of the 2c sites is fully occupied by As and Se (random distribution). With respect to the fractional coordinates of the atoms, the crystal structure determinations based on the data obtained at 25.0 K and 2.3 K did not show significant deviations from ambient temperature results. The temperature dependence of the displacement parameters indicates a static displacement of As on the 2a sites (located on the (0 0 1) planes) for all temperatures. No indications for any occupation of interstitial sites or the presence of vacancies on the Zr (2a) site were found.     

Conception of a new technique in cell cultivation using a lab-on-chip aided miniaturised device with calibratable electrochemical sensors

Continuous electrochemical sensing is often carried out in order to track the growth of cells as an alternative to optical monitoring. Planar sensors and multi-sensor chips are applied in case of adhered growing cells, and usually introduced into lab-on-chip systems. Repeated recalibration is necessary with most chemosensors so far and this limits the operational lifetime of such lab-on-chip systems to a few days. An assembly is presented here that eliminates this disadvantage and enables the monitoring of a long-term cultivation of tissue. Cell cultures and sensor are arranged such that they can be separated or contacted at variable times without having an impact on the growth of the cells. The use of a biocompatible nano-porous membrane is especially important. A multi-well system is described where each well is supplied with a planar multi-sensor chip below the adhered cells to determine (a) pH, (b) glucose, (c) oxygen, and—optionally—impedance, for example during the cultivation of cartilage tissue.     

Simultaneous measurements of heat of hydration and chemical shrinkage on hardening cement pastes

Isothermal calorimetry and chemical shrinkage measurements are two independent techniques used to study the development of hydration in cementitious systems. In this study, calorimetry and chemical shrinkage measurements were combined and simultaneously performed on hydrating cement paste samples. Portland cement pastes with different water to cement ratios and a cement paste containing calcium sulfoaluminate clinker and anhydrite were studied. The combined calorimetry/chemical shrinkage test showed good reproducibility and revealed the different hydration behavior of sealed samples and open samples, i.e., samples exposed to external water during hydration. Large differences between sealed and open samples were observed in a Portland cement paste with low water to cement ratio and in the calcium sulfoaluminate paste; these effects are attributed to self-desiccation of the sealed pastes. Once the setup is fully automatized, it is expected that combined calorimetry/chemical shrinkage measurements can be routinely used for investigating cement hydration.     

Three dimensional film electrode prepared from oppositely charged carbon nanoparticles as efficient enzyme host

Three dimensional carbon film electrodes were prepared from oppositely charged carbon nanoparticles (ca. 9 to 18 nm diameter) by a layer-by-layer approach. This was done by alternative immersion of indium tin oxide plates into suspension of positively and negatively charged particles. A stable film is formed already after single immersion and withdrawal step as confirmed by scanning electron microscopy. Up to ten immersion and withdrawal steps can be used to systematically increase the amount of nanoparticulate carbon material. The capacitive current density and current density of hydrogen peroxide reduction are proportional to the number of immersion and withdrawal steps. The same can be seen for adsorbed redox active 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate). After adsorption of bilirubin oxidase into the film efficient bioelectrocatalytic dioxygen reduction is observed.