A new simple, rapid and sensitive reversed-phase liquid chromatographic method was developed and validated for the simultaneous determination of sulpiride (SUL) and mebeverine Hydrochloride (MEB) in the presence of their impurities and degradation products. The separation of these compounds was achieved within 6 min on a 250 mm, 4.6 mm i.d., 5 m particle size Waters?-C18 column using isocractic mobile phase containing a mixture of acetonitrile and 0.01 M dihydrogenphosphate buffer (45:55) at pH = 4.0. The analysis was performed at a flow rate of 1.0 mL/min with fluorescence-detection at excitation 300 nm and emission at 365 nm. The concentration-response relationship was linear over a concentration range of 10- 100 ng/mL for both MEB and SUL with a limit of detection 0.73 ng/mL and 0.85 ng/mL for MEB and SUL respectively. The proposed method was successfully applied for the analysis of both MEB and SUL in bulk with average recoveries of 100.22 ± 0.757% and 99.96 ± 0.625% respectively, and in commercial tablets with average recoveries of 100.04 ± 0.93% and 100.03 ± 0.376% for MEB and SUL respectively. The proposed method was successfully applied to the determination of MEB metabolite (veratic acid) in real plasma simultaneously with SUL. The mean% recoveries (n = 3) for both MEB metabolite (veratic acid) and SUL were 100.36 ± 2.92 and 99.06 ± 2.11 for spiked human plasma respectively. For real human plasma, the mean% recoveries (n = 3) were and respectively. 相似文献
The skin integrity is essential due to its pivotal role as a biological barrier against external noxious factors. Pentacyclic triterpenes stand as valuable plant-derived natural compounds in the treatment of skin injuries due to their anti-inflammatory, antioxidant, antimicrobial, and healing properties. Consequently, the primary aim of the current investigation was the development as well as the physicochemical and pharmaco-toxicological characterization of betulin- and lupeol-based oleogels (Bet OG and Lup OG) for topical application in skin injuries. The results revealed suitable pH as well as organoleptic, rheological, and textural properties. The penetration and permeation of Bet and Lup oleogels through porcine ear skin as well as the retention of both oleogels in the skin were demonstrated through ex vivo studies. In vitro, Bet OG and Lup OG showed good biocompatibility on HaCaT human immortalized cells. Moreover, Bet OG exerted a potent wound-healing property by stimulating the migration of the HaCaT cells. The in ovo results demonstrated the non-irritative potential of the developed formulations. Additionally, the undertaken in vivo investigation indicated a positive effect of oleogels treatment on skin parameters by increasing skin hydration and decreasing erythema. In conclusion, oleogel formulations are ideal for the local delivery of betulin and lupeol in skin disorders. 相似文献
The structures of AgCu clusters containing 40 atoms are investigated. The most promising structural families (fcc clusters, capped decahedra, and two types of capped polyicosahedra) are singled out by means of global optimization techniques within an atom-atom potential model. Then, representative clusters of each family are relaxed by means of density-functional methods. It is shown that, for a large majority of compositions, a complex interplay of geometric and electronic shell-closure effects stabilizes a specific polyicosahedral family, whose clusters are much lower in energy and present large HOMO-LUMO gaps. Within this family, geometric and quantum effects concur to favor magic structures associated with core-shell chemical ordering and high symmetry, so that these clusters are very promising from the point of view of their optical properties. Our results also suggest a natural growth pathway of AgCu clusters through high-stability polyicosahedral structures. Results for AuCu clusters of the same size are reported for comparison, showing that the interplay of the different effects is highly material specific. 相似文献
Let denote the repartition of the -level correlation measure of the finite set of directions , where is the fixed point and is an integer lattice point in the square . We show that the average of the pair correlation repartition over in a fixed disc converges as . More precisely we prove, for every and , the estimate
We also prove that for each individual point , the -level correlation diverges at any point as , and we give an explicit lower bound for the rate of divergence.
Novel multilayer thin films that consist solely of cross‐linked single component layers are generated by a selective cross‐linking of the poly(vinyl amine) (PVAm) layers in [PVAm/poly(acrylic acid) (PAA)]n thin films constructed either on silica particles or silicon wafers, followed by the removal of PAA. The surface topography of the (PVAm)n multilayer thin films, before and after the adsorption of human serum albumin (HSA), has been studied by atomic force microscopy on the freeze‐dried films. The decrease of the average roughness of the film after the adsorption of HSA showed the protein was adsorbed into the (PVAm)n film making these films potential reservoirs for proteins.
Electron paramagnetic resonance experiments reveal a significant difference between the principal g values (and hence ligand-field parameters) of the ferric cyanide-ligated form of different variants of the protoglobin of Methanosarcina acetivorans (MaPgb) and of horse heart myoglobin (hhMb). The largest principal g value of the ferric cyanide-ligated MaPgb variants is found to be significantly lower than for any of the other globins reported so far. This is at least partially caused by the strong heme distortions as proven by the determination of the hyperfine interaction of the heme nitrogens and mesoprotons. Furthermore, the experiments confirm recent theoretical predictions [Forti, F.; Boechi, L., Bikiel, D., Martí, M.A.; Nardini, M.; Bolognesi, M.; Viappiani, C.; Estrin, D.; Luque, F. J. J. Phys. Chem. B2011, 115, 13771-13780] that Phe(G8)145 plays a crucial role in the ligand modulation in MaPgb. Finally, the influence of the N-terminal 20 amino-acid chain on the heme pocket in these protoglobins is also proven. 相似文献
The so-called “invariance under twisting” for twisted tensor products of algebras is a result stating that, if we start with
a twisted tensor product, under certain circumstances we can “deform” the twisting map and we obtain a new twisted tensor
product, isomorphic to the given one. It was proved before that a number of independent and previously unrelated results from
Hopf algebra theory are particular cases of this theorem. In this article we show that some more results from literature are
particular cases of invariance under twisting, for instance a result of Beattie-Chen-Zhang that implies the Blattner-Montgomery
duality theorem. 相似文献
We extend the Newtonian n-body problem of celestial mechanics to spaces of curvature κ=constant and provide a unified framework for studying the motion. In the 2-dimensional case, we prove the existence of several
classes of relative equilibria, including the Lagrangian and Eulerian solutions for any κ≠0 and the hyperbolic rotations for κ<0. These results lead to a new way of understanding the geometry of the physical space. In the end we prove Saari’s conjecture
when the bodies are on a geodesic that rotates elliptically or hyperbolically. 相似文献
A laycle is the categorical analogue of a lazy cocycle. Twines (introduced by Bruguières) and strong twines (as introduced by the authors) are laycles satisfying some extra conditions. If c is a braiding, the double braiding c2 is always a twine; we prove that it is a strong twine if and only if c satisfies a sort of modified braid relation (we call such cpseudosymmetric, as any symmetric braiding satisfies this relation). It is known that the category of Yetter-Drinfeld modules over a Hopf algebra H is symmetric if and only if H is trivial; we prove that the Yetter-Drinfeld category HYDH over a Hopf algebra H is pseudosymmetric if and only if H is commutative and cocommutative. We introduce as well the Hopf algebraic counterpart of pseudosymmetric braidings under the name pseudotriangular structures and prove that all quasitriangular structures on the 2n+1-dimensional pointed Hopf algebras E(n) are pseudotriangular. We observe that a laycle on a monoidal category induces a so-called pseudotwistor on every algebra in the category, and we obtain some general results (and give some examples) concerning pseudotwistors, inspired by the properties of laycles and twines. 相似文献