Chlortetracycline,a Novel Arf Inhibitor That Decreases the Arf6-Dependent Invasive Properties of Breast Cancer Cells

Breast cancer is a major disease for women worldwide, where mortality is associated with tumour cell dissemination to distant organs. While the number of efficient anticancer therapies increased in the past 20 years, treatments targeting the invasive properties of metastatic tumour cells are still awaited. Various studies analysing invasive breast cancer cell lines have demonstrated that Arf6 is an important player of the migratory and invasive processes. These observations make Arf6 and its regulators potential therapeutic targets. As of today, no drug effective against Arf6 has been identified, with one explanation being that the activation of Arf6 is dependent on the presence of lipid membranes that are rarely included in drug screening. To overcome this issue we have set up a fluorescence-based high throughput screening that follows overtime the activation of Arf6 at the surface of lipid membranes. Using this unique screening assay, we isolated several compounds that affect Arf6 activation, among which the antibiotic chlortetracycline (CTC) appeared to be the most promising. In this report, we describe CTC in vitro biochemical characterization and show that it blocks both the Arf6-stimulated collective migration and cell invasion in a 3D collagen I gel of the invasive breast cancer cell line MDA-MB-231. Thus, CTC appears as a promising hit to target deadly metastatic dissemination and a powerful tool to unravel the molecular mechanisms of Arf6-mediated invasive processes.     

Voltage‐Driven Photoluminescence Modulation of Liquid‐Crystalline Hybridized ZnO Nanoparticles

Liquid‐crystalline hybrid nanomaterials have been obtained by grafting mesogenic units around luminescent ZnO nanocrystals of 5 nm in diameter. Modifying the mesogenic density around the inorganic core allows the modulation of the liquid‐crystalline behavior and its miscibility in commercial liquid crystal (LC). The strong blue photoluminescence observed for the hybrids can be modulated by applying a voltage on a LC cell containing commercial LC and 10 wt % of hybrid.     

Utilizing NDR effect to reduce switching threshold variations in memristive devices

Variations in the switching threshold voltage of memristive devices present significant challenges for their integration into large-scale circuits. In this paper, we propose to address this problem by adding a device exhibiting S-type (N-type) negative differential resistance (NDR) in series (parallel) with memristive devices. The main effect comes from the transition between low- and high-conductivity branches of the NDR device, which leads to a redistribution of the voltage drop inside the device stack, and, as a result, the effective lowering of variations in the switching threshold. The idea is checked experimentally using a TiO_2?x memristive device connected in parallel with a tunnel GaAs diode.     

Fonction L unité d'un groupe de Barsotti-Tate

The purpose of this article is to give a cohomological formula for the unit-root part of the L-function associated to a Barsotti-Tate group G on a scheme S over a field of characteristic p when G extends to some compactification of S. This is an analogue of a part of a conjecture of Katz according to wich the L-function of an F-crystal should be expressed in terms of the p-adic etale sheaf corresponding to the unit-root part of the crystal. In order to carry out this project, we use the technics of [E-LS II] wich require in our case an extension of the Dieudonné crystalline theory ([B-B-M]) to “crystal of level mG” in the sense of Berthelot. We show that the unit-root L-function of the Dieudonné crystal associated to G can be expressed in terms of the syntomic cohomology of the Ext group of G by the constant sheaf.

Received: 24 March 1997 / Revised version: 6 January 1998     

Modelling of pH-dependent electro-osmotic flowsModélisation des variations de flux électro-osmotiques avec le pH.

NaCl-water saturated kaolinite is observed to show pH-dependent electro-osmotic flow. This behaviour is modelled by computing the electro-osmotic flow between parallel planes as a function of their electrical surface charge density. The latter can be related to pH through the physico-chemical properties of the material. In case of kaolinite particles, isomorphic substitution and two surface dissociation reactions allow to predict the pH-dependence of the electrical charge density and thus the variations of electro-osmotic flows with pH. To cite this article: P. Dangla et al., C. R. Mecanique 332 (2004).     

Metal‐Free Synthetic Approach to 3‐Monosubstituted Unsymmetrical 1,2,4,5‐Tetrazines Useful for Bioorthogonal Reactions

A facile, efficient and metal‐free synthetic approach to 3‐monosubstituted unsymmetrical 1,2,4,5‐tetrazines is presented. Dichloromethane (DCM) is for the first time recognized as a novel reagent in the synthetic chemistry of tetrazines. Using this novel approach 11 3‐aryl/alkyl 1,2,4,5‐tetrazines were prepared in excellent yields (up to 75 %). The mechanism of this new reaction, including the role of DCM in the tetrazine ring formation, has been investigated by ¹³C labeling of DCM, and is also presented and discussed as well as the photophysical and electrochemical properties.     

Influence of the crystallization on the molecular mobility and ionic DC conductivity behaviors of relaxor ferroelectric P(VDF‐TrFE‐CTFE) terpolymers

The dynamics of semicrystalline poly(vinylidene fluoride‐trifluoroethylene‐chlorotrifluoroethylene) (P(VDF‐TrFE‐CTFE)) terpolymers were fully investigated as a function of temperature and frequency, by means of broadband dielectric spectroscopy. Four types of relaxation regimes were observed over the full dielectric spectroscopy, namely (second) relaxation in the sub‐glass state, (segmental dynamics) in the rubbery state, the Curie transition and space charge carrier motion at high temperatures. Constrained segmental dynamics were observed in the terpolymer containing the highest crystalline fraction for which a narrow relaxation time distribution was found. These results indicate a decreasing average size of the cooperative rearranging region over the crystalline fraction. A decrease of the strength index values also implied a more fragile behavior for terpolymers with a higher degree of crystallinity. An exceptional increase in dielectric strength was found as the crystallinity increased. This behavior could be explained by an enhanced interphase (constrained amorphous phase). The Curie transition showed an accelerating relaxation rate for a more fragile terpolymer. Moreover, a motion of the space charge carrier ions was observed in the higher temperature range. Finally, there was evidence that the segmental dynamics in the amorphous phase was responsible for the motion of the space charge carrier ions. © 2016 Wiley Periodicals, Inc. J. Polym. Sci., Part B: Polym. Phys. 2016 , 54, 1645–1657     

Dynamics and structure of unstably stratified homogeneous turbulence

We characterise the properties of unstably stratified homogeneous turbulence by means of high-resolution direct numerical simulations and a two-point statistical spectral model based on a quasi-normal closure proposed by Burlot et al. Both approaches agree very well regarding the evolution of one- and two-point turbulent statistics, showing that the model is valid at even higher Reynolds numbers than previously considered. From a parametric study with different initial conditions, we confirm that the energy distribution at large scale influences strongly the late time dynamics of the flow. In particular, we assess the existence of backscatter transfer of energy, and evaluate its role in the growth rate of several turbulent quantities. Moreover, thanks to the statistical model, we analyse the scale-by-scale anisotropy of the flow through the decomposition of turbulent spectra in terms of directional anisotropy and polarisation anisotropy, for a refined characterisation of the structure of the flow which is strongly anisotropic in the large scales. This also allows us to study how isotropy is restored in the inertial scales.     

Synthetic Approaches to Trifluoromethoxy‐Substituted Compounds

Because of the unique properties of the trifluoromethoxy group, molecules bearing this moiety will find applications in various fields, particularly in the life sciences. However, despite the great interest in this functional group, only a small number of trifluoromethoxylated molecules are currently synthetically accessible. Over the last few years, several innovative and promising strategies for the synthesis of trifluoromethoxylated compounds have been described. This Minireview discusses these existing methods with a particular focus on more recent advances.