Long-term characteristics of analogical processing in high-school students with high fluid intelligence: an fMRI study

Intelligence is known to predict scholastic achievement and enables high performance in cognitive tasks. Fluid intelligence is strongly related to analogical reasoning abilities, which are fundamental to mathematical thinking. Geometric analogical reasoning is a prototypical measure of fluid intelligence. However, the cerebral correlates of geometric analogical reasoning and their developmental modulation over time are still rarely investigated. We report a 1-year follow-up functional magnetic resonance imaging study of a geometric analogical reasoning task in high fluid intelligence high-school students. This study was designed to characterise the cerebral correlates of geometric analogical reasoning and to improve our knowledge about the impact of general cognitive development on behavioural performance and on cerebral mechanisms underlying geometric analogical reasoning in adolescents. Our data indicate that a fronto-parietal network comprising the left and right parietal lobes and the left middle frontal gyrus was equally modulated by task difficulty at both measuring time points. At the behavioural level, however, participants showed improvements in performance at the second measuring time point. The behavioural improvements point to a more efficient task processing. As this is not accompanied by differential recruitment of fronto-parietal brain regions, the data suggest an increase in neural efficiency for these brain regions.     

A polynomial time approximation algorithm for the two-commodity splittable flow problem

We consider a generalization of the unsplittable maximum two-commodity flow problem on undirected graphs where each commodity ${i \in \{1, 2\}}$ can be split into a bounded number k _i of equally-sized chunks that can be routed on different paths. We show that in contrast to the single-commodity case this problem is NP-hard, and hard to approximate to within a factor of α > 1/2. We present a polynomial time 1/2-approximation algorithm for the case of uniform chunk size over both commodities and show that for even k _i and a mild cut condition it can be modified to yield an exact method. The uniform case can be used to derive a 1/4-approximation for the maximum concurrent (k ₁, k ₂)-splittable flow without chunk size restrictions for fixed demand ratios.     

Harn: Formoltitration

Ohne ZusammenfassungVergl. auch diese Zeitschrift47, 547;49, 66.     

Enzymatic modification of lignocellulosic substances for the production of fiberboards

The object of the research is the development of enzyme systems for the enzymatic modification of wood fiber materials as well as fiber materials from annual plants for the production of glue-free fiberboards. The project is aimed first at the process development for the enzymatic modification and second at the development of cellulase/hemicellulase complexes on the basis of stillage as a substrate and inducer for the enzymatic modification. The results demonstrate that it is possible to substitute synthetic resins by means of activation and biocatalytic cross-linking of fibers with hydrolytic enzyme systems, in particular, for the production of medium-density fiberboards. The article is published in the original.     

Optimized and far-red-emitting variants of fluorescent protein eqFP611

Fluorescent proteins (FPs) emitting in the far-red region of the spectrum are highly advantageous for whole-body imaging applications because scattering and absorption of long-wavelength light is markedly reduced in tissue. We characterized variants of the red fluorescent protein eqFP611 with bright fluorescence emission shifted up to 639 nm. The additional red shift is caused by a trans-cis isomerization of the chromophore. The equilibrium between the trans and cis conformations is strongly influenced by amino acid residues 143 and 158. Pseudo monomeric tags were obtained by further genetic engineering. For the red chromophores of eqFP611 variants, molar extinction coefficients of up to approximately 150,000 were determined by an approach that is not affected by the presence of molecules with nonfunctional red chromophores. The bright fluorescence makes the red-shifted eqFP611 variants promising lead structures for the development of near-infrared fluorescent markers. The red fluorescent proteins performed well in cell biological applications, including two-photon imaging.     

Structure-based engineering of strictosidine synthase: auxiliary for alkaloid libraries

The highly substrate-specific strictosidine synthase (EC 4.3.3.2) catalyzes the biological Pictet-Spengler condensation between tryptamine and secologanin, leading to the synthesis of about 2000 monoterpenoid indole alkaloids in higher plants. The crystal structure of Rauvolfia serpentina strictosidine synthase (STR1) in complex with strictosidine has been elucidated here, allowing the rational site-directed mutation of the active center of STR1 and resulting in modulation of its substrate acceptance. Here, we report on the rational redesign of STR1 by generation of a Val208Ala mutant, further describing the influence on substrate acceptance and the enzyme-catalyzed synthesis of 10-methyl- and 10-methoxystrictosidines. Based on the addition of strictosidine to a crude strictosidine glucosidase preparation from Catharanthus cells, a combined chemoenzymatic approach to generating large alkaloid libraries for future pharmacological screenings is presented.