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181.
Ihling C Berger K Höfliger MM Führer D Beck-Sickinger AG Sinz A 《Rapid communications in mass spectrometry : RCM》2003,17(12):1240-1246
Fourier transform ion-cyclotron resonance (FTICR) mass spectrometry offers several advantages for the analysis of biological samples, including excellent mass resolution, ultra-high mass measurement accuracy, high sensitivity, and wide mass range. We report the application of a nano-HPLC system coupled to an FTICR mass spectrometer equipped with nanoelectrospray source (nano-HPLC/nano-ESI-FTICRMS) for proteome analysis. Protein identification in proteomics is usually conducted by accurately determining peptide masses resulting from enzymatic protein digests and comparing them with theoretically digested protein sequences from databases. A tryptic in-solution digest of bovine serum albumin was used to optimize experimental conditions and data processing. Spots from Coomassie Blue and silver-stained two-dimensional (2D) gels of human thyroid tissue were excised, in-gel digested with trypsin, and subsequently analyzed by nano-HPLC/nano-ESI-FTICRMS. Additionally, we analyzed 1D-gel bands of membrane preparations of COS-6 cells from African green monkey kidney as an example of more complex protein mixtures. Nano-HPLC was performed using 1-mm reverse-phase C-18 columns for pre-concentration of the samples and reverse-phase C-18 capillary columns for separation, applying water/acetonitrile gradient elution conditions at flow rates of 200 nL/min. Mass measurement accuracies smaller than 3 ppm were routinely obtained. Different methods for processing the raw data were compared in order to identify a maximum number of peptides with the highest possible degree of automation. Parallel identification of proteins from complex mixtures down to low-femtomole levels makes nano-HPLC/nano-ESI-FTICRMS an attractive approach for proteome analysis. 相似文献
182.
S.V. Dordevic D.N. Basov R.C. Dynes B. Ruzicka V. Vescoli L. Degiorgi H. Berger R. Gaál L. Forró E. Bucher 《The European Physical Journal B - Condensed Matter and Complex Systems》2003,33(1):15-23
We present a comprehensive analysis of the optical constants of the two-dimensional dichalcogenide materials 2
H
-
TaSe
2
and 2
H
-
NbSe
2
, in an attempt to address the physics of two-dimensional correlated systems. The title compounds were studied over several
decades in frequency, from the far-infrared to the ultraviolet. Measurements with linearly polarized light have allowed us
to obtain both the in-plane and out-of-plane components of the conductivity tensor. Although the electromagnetic response
of dichalcogenides is strongly anisotropic, both the in-plane and out-of-plane components of the conductivity tensor share
many common features, including the presence of a well-defined metallic component, as well as a “mid-infrared band”. We discuss
the implications of these results in the context of the spectroscopic results of other classes of low-dimensional conductors
such as the high-temperature superconducting cuprates. In particular, the analysis of the redistribution of the spectral weight
as a function of temperature, as well as the behavior of the quasiparticles relaxation rate, points to significant distinctions
between the charge dynamics of dichalcogenides and other classes of low dimensional conductors.
Received 28 October 2002 / Received in final form 10 March 2003 Published online 23 May 2003
RID="a"
ID="a"e-mail: degiorgi@solid.phys.ethz.ch 相似文献
183.
Berger EL Harris BW Kaplan DE Sullivan Z Tait TM Wagner CE 《Physical review letters》2001,86(19):4231-4234
A long-standing discrepancy between the bottom-quark production cross section and predictions of perturbative quantum chromodynamics is addressed. We show that pair production of light gluinos, of mass 12 to 16 GeV, with two-body decays into bottom quarks and light bottom squarks, yields a bottom-quark production rate in agreement with hadron collider data. We examine constraints on this scenario from low-energy data and make predictions that may be tested at the next run of the Fermilab Tevatron collider. 相似文献
184.
Camus E Talmant M Berger G Laugier P 《The Journal of the Acoustical Society of America》2000,108(6):3058-3065
Ultrasonic wave propagation in human cortical bone has been investigated in vitro using the so-called axial transmission technique. This technique, which relies on velocity measurement of the first arriving signal, has been used in earlier investigations to study bone status during fracture healing or osteoporosis. Two quasi-point-source elements, one transmitter and one receiver (central frequency 0.5 MHz), were used to generate a wide ultrasonic beam, part of which strikes the sample surface at the longitudinal critical angle, and to receive the signals reflected from the sample surface. The analysis of the field reflected from a fluid-solid interface for an incident spherical wave predicts the existence of a lateral wave propagating along the sample surface at a velocity close to the longitudinal velocity, in addition to the ordinary reflected wave and vibration modes. The transducer-sample and the transmitter-receiver distances were chosen such that the lateral wave is the first arriving signal. Validation of the measuring technique was performed on test materials and was followed by experiments on human cortical bones. Experimental results (arrival time and velocity) strongly suggest that the first detected signal corresponds to the lateral wave predicted by theory. 相似文献
185.
Berger JR Martin PA McCaffery SJ 《The Journal of the Acoustical Society of America》2000,107(3):1161-1167
In this paper, the propagation of time-harmonic torsional waves in composite elastic cylinders is investigated. An imperfect interface is considered where tractions are continuous across the interface and the displacement jump is proportional to the stress acting on the interface. A frequency equation is derived for the rod and dispersion curves of normalized frequency as a function of normalized wave number for elastic bimaterials with varying values for the interface constant F are presented. The analysis is shown to recover the dispersion curves for a bimaterial rod with a perfect (welded) interface (F = 0), and has the correct limiting behavior for large F. It is shown that the modes, at any given frequency, are orthogonal, and it is outlined how the problem of reflection of a torsional mode by a planar defect (such as a circumferential crack) can be treated. 相似文献
186.
Forro L Gaal R Berger H Fazekas P Penc K Kezsmarki I Mihaly G 《Physical review letters》2000,84(9):1938-1941
We report on experiments giving evidence for quantum effects of electromagnetic flux in barium alumosilicate glass. In contrast to expectation, below 100 mK the dielectric response becomes sensitive to magnetic fields. The experimental findings include both lifting of the dielectric saturation by weak magnetic fields and oscillations of the dielectric response in the low temperature resonant regime. As the origin of these effects we suggest that the magnetic induction field violates the time reversal invariance leading to a flux periodicity in the energy levels of tunneling systems. At low temperatures, this effect is strongly enhanced by the interaction between tunneling systems and thus becomes measurable. 相似文献
187.
Borisenko SV Golden MS Legner S Pichler T Durr C Knupfer M Fink J Yang G Abell S Berger H 《Physical review letters》2000,84(19):4453-4456
On the basis of angle-scanned photoemission data recorded using unpolarized radiation, with high (E,k) resolution, and an extremely dense sampling of k space, we resolve the current controversy regarding the normal state Fermi surface (FS) in Bi(2)Sr(2)CaCu(2)O(8+delta). The true picture is simple, self-consistent, and robust: the FS is holelike, with the form of rounded tubes centered on the corners of the Brillouin zone. Two further types of features are also clearly observed: shadow FSs, which are most likely to be due to short range antiferromagnetic spin correlations, and diffraction replicas of the main FS caused by passage of the photoelectrons through the modulated Bi-O planes. 相似文献
188.
Smith JG Alexander JP Baker R Bebek C Berger BE Berkelman K Blanc F Boisvert V Cassel DG Dickson M Drell PS Ecklund KM Ehrlich R Foland AD Gaidarev P Gibbons L Gittelman B Gray SW Hartill DL Heltsley BK Hopman PI Jones CD Kreinick DL Lohner M Magerkurth A Meyer TO 《Physical review letters》2000,84(7):1393-1397
We present the first observation of the decay B-->J/psistraight phiK. Using 9.6x10(6) B&Bmacr; meson pairs collected with the CLEO detector, we have observed ten fully reconstructed B-->J/psistraight phiK candidates, whereas the estimated background is 0.5+/-0.2 event. We obtain a branching fraction of B(B-->J/psistraight phiK) = (8. 8(+3.5)(-3.0)[stat]+/-1.3[syst])x10(-5). This is the first observed B meson decay requiring the creation of an additional s&smacr; quark pair. 相似文献
189.
Prakhov S Tippens WB Allgower C Bekrenev V Berger E Briscoe WJ Clajus M Comfort JR Craig K Grosnick D Huber GM Isenhower D Knecht N Koetke D Koulbardis A Kozlenko N Kruglov S Kycia T Lolos GJ Lopatin I Manley DM Marusic A Manweiler R McDonald S Nefkens BM Olmsted J 《Physical review letters》2000,84(21):4802-4805
We report the first determination of the upper limit for the branching ratio of the CP forbidden decay eta-->4pi(0). No events were observed in a sample of 3.0x10(7) eta decays. The experiment was performed with the Crystal Ball multiphoton spectrometer installed in a separated pi(-) beam at the AGS (Alternating Gradient Synchrotron). At the 90% confidence limit, B(eta-->4pi(0))=6. 9x10(-7). 相似文献
190.
This paper reports the formation and properties of nano-composite pyroelectric thin films. They consist of pyroelectric triglycine sulfate (TGS) single-crystal nano-rods grown inside a highly dense array of alumina pores (about 65 nm diameter and density of 1011 cm−2). The nucleation and growth of the TGS single crystals are obtained by precipitation from a supersaturated aqueous solution. Nucleation is preferred only at the bottom of the pores due to a tight control of temperature, composition and pore diameter. Growth of single crystals with preferred crystallographic orientation is obtained with the aid of an applied electric field. Various crystallographic orientations (1 0 0) (−1 1 0) (−1 2 0) are obtained separately as a single preferred orientation by changing the amplitude of the electric field during crystal growth. The films exhibit ferroelectric behavior. 相似文献