基于核酸适配体的微流控芯片酶反应器用于蛋白质的分析

将核酸适配体作为胰蛋白酶固定化介质,制备了一种新型的微流控芯片酶反应器,并与高效液相色谱-串联质谱联用,搭建了在线分析平台;分别使用标准蛋白及混合蛋白样品对芯片的酶解效率及联用平台的分析能力进行了初步评价。结果表明,5 ng肌红蛋白经该平台分析后肽段覆盖率可达到37%;对500 ng混合蛋白进行3次平行分析,肽段覆盖率及相对标准偏差分别为44.3%、6.5%(牛血清白蛋白), 65.0%、2.7%(肌红蛋白)和62.0%、5.6%(细胞色素c);初步实验表明,该在线分析平台具有检测灵敏度高、重现性好、酶解效率高的特点,有望在蛋白质组学分析中发挥重要作用。     

微波等离子体化学气相沉积法低温制备直纳米碳管膜

Among the three main methods for the synthesis of carbon nanotubes (CNTs)， chemical vapor deposition (CVD) has received a great deal of attention since CNTs can be synthesized at significantly low temperature. Plasma chemical vapor deposition methods can synthesize CNTs at lower temperature than thermal CVD. But in the usual catalytic growth of CNTs by CVD， CNTs are often tangled together and have some defects. These will limit the property research and potential applications. How to synthesize the straight CNTs at low temperature becomes a challenging issue. In this letter， straight carbon nanotube (CNT) films were achieved by microwave plasma chemical vapor deposition (MWPCVD) catalyzed by round Fe-Co-Ni alloy particles on Ni substrate at 610℃. It was found that， in our experimental condition， the uniform growth rate along the circumference of round alloy particles plays a very important role in the growth of straight CNT films. And because the substrate is conducting， the straight CNT films grown at low temperature may have the benefit for property research and offer the possibility to use them in the future applications.     

Self-Dual Yang-Mills Theory as a Two-Dimensional Chiral Model and Its Conservation Laws

The self-dual Yang-Mills field theories in two-dimensional representations are formulated. The Biicklund transformation and the conservation law are given in the framework of the prolongation theory. Furthermore, we derive the conserved current algebras which are in essence the Yang-Baxter relations.     

ChemInform Abstract: The Infrared Spectrum of F-·H2O.

The geometry and vibrational frequencies of the F-·H₂O system are determined by ab initio calculations (SCF and CISD levels of theory).     

FINITE ELEMENT SOLUTIONS OF LAMINAR AND TURBULENT MIXED CONVECTION IN A DRIVEN CAVITY

This paper presents laminar and turbulent mixed convection solutions of a driven cavity flow using the finite element method. For the laminar flow, distributions of velocity and temperature with and without the effect of buoyancy force are presented and compared. For the turbulent flow, governing partial differential equations of the thermal turbulence two-equati on model and kinetic turbulence two-equation model are used. Corresponding results such as kinetic eddy diffusivity, kinetic eddy energy, thermal eddy energy and their dissipations are presented.     

The effects of external optical feedback on the power penalty of DFB-LD modules for 2.5Gbs−1 optical transmission systems

We report the effects of external optical feedback on the power penalty of commercial distributed feedback laser diode (DFB-LD) modules for 2.5Gbs–1 optical transmission systems. External optical feedback presented to the DFB-LD modules causes the excitation of external cavity modes, resulting in increased relative intensity noise (RIN) and intensity noise ripples at low frequency region below 500MHz. For a 10–10 bit error rate (BER), the minimum power penalty is as much as 1.25dB for a feedback ratio of –8.8dB. An excess power penalty of 0.5dB per 3dB increase in the feedback ratio was also empirically obtained. We suggest that optical isolators in 2.5Gbs–1 DFB-LD modules used in conventional optical transmission systems or WDM systems must have a peak isolation ratio of better than 54.5dB, instead of the previously recommended 30dB, for negligible power penalty induced by external optical feedback.     

TIME-AVERAGED SPONTANEOUS POWER RADIATION IN PLASMA FOCUS

The time-averaged spontaneous radiation power and its spectrum are derived analytically. The collective mode is taken to be electron-cyclotron mode driven by the relativistic electron beam existing in the unstable and late phases of plasma focus. The infrared range radiation calculated is in agreement with experimental observation.     

PARAMETRIC STUDY FOR AN EFFICIENT MESHLESS METHOD IN VIBRATION ANALYSIS

Based on the moving least-squares (MLS) approach, an efficient meshless method is employed to generate the displacement functions for vibration analysis of elastic bodies. The equation of motion is established by following the standard procedure and the boundary conditions are imposed by applying penalty functions. As the displacement functions are expressed in terms of weight functions, the accuracy will depend on the parameters of the weight functions. Therefore, a parametric study is carried out to determine the best values for these parameters. To demonstrate the accuracy, modal analyses of the beams and plates with different boundaries have been carried out. In addition, the responses of these structures under dynamic excitation have been analyzed. The examples include simply supported beams subjected to sudden excitations and simply supported plates subjected to initial displacements.