Engineering the Big Chill: The Story of JLab’s Central Helium Liquefier

This article tells the story of the Central Helium Liquefier (CHL) at the Thomas Jefferson National Accelerator Facility (JLab), one of the US National Laboratories. JLab’s successful superconducting radio frequency accelerator was only possible because a group of JLab engineers successfully tackled a complex of difficulties to build a cryogenic system that included the CHL, a task that required advancing the frontier of cryogenic technology. Ultimately, these cryogenic advances were applied far beyond JLab to the benefit of cutting-edge programs at other US national laboratories (Oak Ridge, Brookhaven, and the Facility for Rare Isotope Beams at MSU) as well as NASA. This innovation story dramatizes the sort of engineer-driven technological problem solving that allows the successful launch and operation of experimental projects. Along the way, the CHL story also provides an important addition to our understanding of the role played by engineers and industry in creating knowledge at physics laboratories.     

Convenient Access to Fluorescent Probes by Chemoselective Acylation of Hydrazinopeptides: Application to the Synthesis of the First Far‐Red Ligand for Apelin Receptor Imaging

Herein, we develop a convenient method to facilitate the solution‐phase fluorescent labelling of peptides based on the chemoselective acylation of α‐hydrazinopeptides. This approach combines the advantages of using commercially available amine‐reactive dyes and very mild conditions, which are fully compatible with the chemical sensitivity of the dyes. The usefulness of this approach was demonstrated by the labelling of apelin‐13 peptide. Various fluorescent probes were readily synthesized, enabling the rapid optimization of their affinities for the apelin receptor. Thus, the first far‐red fluorescent ligand with sub‐nanomolar affinity for the apelin receptor was characterized and shown to track the receptor efficiently in living cells by fluorescence confocal microscopy.     

High-Pressure NiAs-Type Modification of FeN

The combination of laser-heated diamond anvil cells and synchrotron Mössbauer source spectroscopy were used to investigate high-temperature high-pressure chemical reactions of iron and iron nitride Fe₂N with nitrogen. At pressures between 10 and 45 GPa, significant magnetic hyperfine splitting indicated compound formation after annealing at 1300 K. Subsequent in situ X-ray diffraction reveals a new modification of FeN with NiAs-type crystal structure, as also rationalized by first-principles total-energy and chemical-bonding studies.     

Recovering the Geometry of a Flat Spacetime from Background Radiation

We consider globally hyperbolic flat spacetimes in 2 + 1 and 3 + 1 dimensions, in which a uniform light signal is emitted on the r-level surface of the cosmological time for r → 0. We show that the frequency shift of this signal, as perceived by a fixed observer, is a well-defined, bounded function which is generally not continuous. This defines a model with anisotropic background radiation that contains information about initial singularity of the spacetime. In dimension 2 + 1, we show that this observed frequency shift function is stable under suitable perturbations of the spacetime, and that, under certain conditions, it contains sufficient information to recover its geometry and topology. We compute an approximation of this frequency shift function for a few simple examples.     

Maximum likelihood estimator consistency for a ballistic random walk in a parametric random environment

We consider a one dimensional ballistic random walk evolving in an i.i.d. parametric random environment. We provide a maximum likelihood estimation procedure of the parameters based on a single observation of the path till the time it reaches a distant site, and prove that the estimator is consistent as the distant site tends to infinity. Our main tool consists in using the link between random walks and branching processes in random environments and explicitly characterising the limiting distribution of the process that arises. We also explore the numerical performance of our estimation procedure.     

A new highly adaptable design of shear-flow device for orientation of macromolecules for Linear Dichroism (LD) measurement

This article presents a new design of flow-orientation device for the study of bio-macromolecules, including DNA and protein complexes, as well as aggregates such as amyloid fibrils and liposome membranes, using Linear Dichroism (LD) spectroscopy. The design provides a number of technical advantages that should make the device inexpensive to manufacture, easier to use and more reliable than existing techniques. The degree of orientation achieved is of the same order of magnitude as that of the commonly used concentric cylinders Couette flow cell, however, since the device exploits a set of flat strain-free quartz plates, a number of problems associated with refraction and birefringence of light are eliminated, increasing the sensitivity and accuracy of measurement. The device provides similar shear rates to those of the Couette cell but is superior in that the shear rate is constant across the gap. Other major advantages of the design is the possibility to change parts and vary sample volume and path length easily and at a low cost.