We present a new algorithm for estimating parameters in reaction–diffusion systems that display pattern formation via the mechanism of diffusion-driven instability. A Modified Discrete Optimal Control Algorithm (MDOCA) is illustrated with the Schnakenberg and Gierer–Meinhardt reaction–diffusion systems using PDE constrained optimization techniques. The MDOCA algorithm is a modification of a standard variable step gradient algorithm that yields a huge saving in computational cost. The results of numerical experiments demonstrate that the algorithm accurately estimated key parameters associated with stationary target functions generated from the models themselves. Furthermore, the robustness of the algorithm was verified by performing experiments with target functions perturbed with various levels of additive noise. The MDOCA algorithm could have important applications in the mathematical modeling of realistic Turing systems when experimental data are available. 相似文献
Dissociative nuclear motion in core-excited molecular states leads to a splitting of the fragment Auger lines: the Auger-Doppler effect. We present here for the first time experimental evidence for an Auger-Doppler effect following F1s → a(1g)* inner-shell excitation by circularly polarized x rays in SF(6). In spite of a uniform distribution of the dissociating S-F bonds near the polarization plane of the light, the intersection between the subpopulation of molecules selected by the core excitation with the cone of dissociation induces a strong anisotropy in the distribution of the S-F bonds that contributes to the scattering profile measured in the polarization plane. 相似文献
We consider a restricted four-body problem, with a precise hierarchy between the bodies: two larger bodies and a smaller one, all three of oblate shape, and a fourth, infinitesimal body, in the neighborhood of the smaller of the three bodies. The three heavy bodies are assumed to move in a plane under their mutual gravity, and the fourth body to move in the three-dimensional space under the gravitational influence of the three heavy bodies, but without affecting them. We first find that the triangular central configuration of the three heavy oblate bodies is a scalene triangle (rather than an equilateral triangle as in the point mass case). Then, assuming that these three bodies are in such a central configuration, we perform a Hill approximation of the equations of motion describing the dynamics of the infinitesimal body in a neighborhood of the smaller body. Through the use of Hill’s variables and a limiting procedure, this approximation amounts to sending the two larger bodies to infinity. Finally, for the Hill approximation, we find the equilibrium points for the motion of the infinitesimal body and determine their stability. As a motivating example, we identify the three heavy bodies with the Sun, Jupiter, and the Jupiter’s Trojan asteroid Hektor, which are assumed to move in a triangular central configuration. Then, we consider the dynamics of Hektor’s moonlet Skamandrios.
Stilbene cleaving oxygenases (SCOs), also known as lignostilbene-α,β-dioxygenases (LSDs) mediate the oxidative cleavage of the olefinic double bonds of lignin-derived intermediate phenolic stilbenes, yielding small modified benzaldehyde compounds. SCOs represent one branch of the larger carotenoid cleavage oxygenases family. Here, we describe the structural and functional characterization of an SCO-like enzyme from the soil-born, bio-control agent Pseudomonas brassicacearum.
Methods
In vitro and in vivo assays relying on visual inspection, spectrophotometric quantification, as well as liquid-chormatographic and mass spectrometric characterization were applied for functional evaluation of the enzyme. X-ray crystallographic analyses and in silico modeling were applied for structural investigations.
Results
In vitro assays demonstrated preferential cleavage of resveratrol, while in vivo analyses detected putative cleavage of the straight chain carotenoid, lycopene. A high-resolution structure containing the seven-bladed β-propeller fold and conserved 4-His-Fe unit at the catalytic site, was obtained. Comparative structural alignments, as well as in silico modelling and docking, highlight potential molecular factors contributing to both the primary in vitro activity against resveratrol, as well as the putative subsidiary activities against carotenoids in vivo, for future validation.
Conclusions
The findings reported here provide validation of the SCO structure, and highlight enigmatic points with respect to the potential effect of the enzyme’s molecular environment on substrate specificities for future investigation.
Cholesterylbutyrate (Chol-but) was chosen as a prodrug of butyric acid. Butyrate is not often used in vivo because its half-life is very short and therefore too large amounts of the drug would be necessary for its efficacy. In the last few years butyric acid's anti-inflammatory properties and its inhibitory activity towards histone deacetylases have been widely studied, mainly in vitro. Solid Lipid Nanoparticles (SLNs), whose lipid matrix is Chol-but, were prepared to evaluate the delivery system of Chol-but as a prodrug and to test its efficacy in vitro and in vivo. Chol-but SLNs were prepared using the microemulsion method; their average diameter is on the order of 100-150 nm and their shape is spherical. The antineoplastic effects of Chol-but SLNs were assessed in vitro on different cancer cell lines and in vivo on a rat intracerebral glioma model. The anti-inflammatory activity was evaluated on adhesion of polymorphonuclear cells to vascular endothelial cells. In the review we will present data on Chol-but SLNs in vitro and in vivo experiments, discussing the possible utilisation of nanoparticles for the delivery of prodrugs for neoplastic and chronic inflammatory diseases. 相似文献
A selective and sensitive method is presented for biogenic amines (BA) determination. The novelty consists in coupling a highly selective electrochemical biosensor to a weak acid cation-exchange column for online detection of amines. A bienzyme design, based on a recently isolated amine oxidase from grass pea and commercial horseradish peroxidase, was used for the biosensor construction. The enzymes were co-immobilized on the surface of a graphite electrode together with the electrochemical mediator (Os-redox polymer). The electrochemical detection was performed at a low applied potential (?50 mV vs. Ag/AgCl, KCl0.1 M), where biases from interferences are minimal. The separation and determination of six BA, with relevance in food analysis (tyramine, putrescine, cadaverine, histamine, agmatine and spermidine), were investigated. Irrespective of the BA nature, the amine oxidase-based biosensor showed a linear response up to 5 mM, and its sensitivity decreases in the following order: cadaverine, putrescine, spermidine, agmatine, histamine and tyramine. The approach was used to estimate the BA content in fish samples, after their extraction with methanesulfonic acid. 相似文献
Up to now the content of the organic halides in sediments was measured as extractable organic halides compounds by different
methods including microcoulometric titration one. We describe a procedure for microcoulometric determination of total organic
halide pollutants by using a direct combustion of the sediments into the microcoulometric system furnace. An accelerated removal
of the inorganic chlorides by reaction with potassium nitrate using ultrasonic radiation was attained. The procedure was validated
for analysis of bottom sediments from natural and anthropogenic sources. The repeatability RSD = 8.5%, the expanded relative
uncertainty U (n = 7, P = 95%, k = 2) = 6.4%, the reproducibility RSD was within the range 10.0–7.3%, the average recovery R = 97.6% and method LOD = 11 mg kg−1 Cl.
Correspondence: Zara V. Aneva, Analytical Department, University “Prof. Dr. Assen Zlatarov”, Nr. 1 Prof. Yakimov street, 8010
Bourgas, Bulgaria 相似文献
A bimetallic coordination polymer, infinity (2)[{LNi (II)Co (II)}(dca) 2], has been constructed from heterobinuclear [Ni (II)Co (II)] nodes and dicyanamido spacers [L (2-) is the dianion of the Schiff base resulting from the 2:1 condensation of 3-methoxysalicyladehyde with 1,3-propanediamine; L (2-) = N, N'-propylenebis(3-methoxysalycilideneiminato)]. The intranode Co (II)-Ni (II) interaction was found to be ferromagnetic because of the orthogonality of the magnetic orbitals. Below 12 K, the onset of the canted ferromagnetic ordering is observed. 相似文献
The spinel-type ferrites NiFe2O4 and Zn0.5Ni0.5Fe2O4 modified by lanthanide ions Eu3+ and Tb3+ were prepared by a sol—gel process with propylene oxide as a gelating agent. The phase homogeneity of the samples was tested by XRD and Mössbauer spectroscopy. Transmission electronic microscopy used for characterisation of the morphology of the samples revealed nanosized powdered samples with a narrow distribution of particle sizes. It was noted that the presence of Ln3+ ions influenced the magnetic properties of nanosized NiFe2O4 and Ni0.5Zn0.5Fe2O4 ferrites. The dependence of the magnetic properties of the samples on the rare-earth doping may be explained by the different grain sizes. The saturation magnetisation tends to decrease with increasing rare-earth doping and decreasing crystallite size. A similar trend was observed for the coercive field, with the exception of the Tb3+-doped Zn0.5Ni0.5Fe2O4 where it remained the same as in the pure ferrite. 相似文献