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51.
Patterning multiple cell types is a critical step for engineering functional tissues, but few methods provide three-dimensional positioning at the cellular length scale. Here, we present a "bottom-up" approach for fabricating multicellular tissue constructs that utilizes DNA-templated assembly of 3D cell-laden hydrogel microtissues. A flow focusing-generated emulsion of photopolymerizable prepolymer is used to produce 100 μm monodisperse microtissues at a rate of 100 Hz (10(5) h(-1)). Multiple cell types, including suspension and adherently cultured cells, can be encapsulated into the microtissues with high viability (~97%). We then use a DNA coding scheme to self-assemble microtissues "bottom-up" from a template that is defined using "top-down" techniques. The microtissues are derivatized with single-stranded DNA using a biotin-streptavidin linkage to the polymer network, and are assembled by sequence-specific hybridization onto spotted DNA microarrays. Using orthogonal DNA codes, we achieve multiplexed patterning of multiple microtissue types with high binding efficiency and >90% patterning specificity. Finally, we demonstrate the ability to organize multicomponent constructs composed of epithelial and mesenchymal microtissues while preserving each cell type in a 3D microenvironment. The combination of high throughput microtissue generation with scalable surface-templated assembly offers the potential to dissect mechanisms of cell-cell interaction in three dimensions in healthy and diseased states, as well as provides a framework for templated assembly of larger structures for implantation. 相似文献
52.
The Infrared spectrum of solid chlorotrifluoromethane (CF3Cl) has been studied and compared with that of matrix-isolated CF3Cl. Crystal field splitting patterns of the strongest modes ν1 and ν4 suggest the crystal structure to be orthorhombic with a C2v factor group and two molecules per unit cell located on Cs sites. 相似文献
53.
We study hereL 1-convergence of a complex trigonometric sum and obtain a new necessary and sufficient condition for theL 1-convergence of Fourier series. 相似文献
54.
Surendran Ramasamy Abdul Rahman Mohamed Subhash Bhatia 《Reaction Kinetics and Catalysis Letters》2002,75(2):353-358
Oxidative coupling of methane for the production of ethylene was studied over Li-Ni/MgO catalyst in a fixed bed reactor. The influences of important reaction parameters such as temperature (T), methane/oxygen ratio (CH4/O2) in feed and space velocity of reactants (V/mcat) were studied over the conversion of methane, yields of ethylene and ethane and selectivity of ethylene formation. The reaction conditions were varied as 650 < T < 850oC, 0.83 x 10-6 < V/mcat < 2.92 x 10-6 m3/g s and 1 < CH4/O2 ratio < 8. 相似文献
55.
Characterization of microporous membranes for use in membrane contactors 总被引:11,自引:0,他引:11
S. B. Iversen V. K. Bhatia K. Dam-Johansen G. Jonsson 《Journal of membrane science》1997,130(1-2):205-217
Methods of selecting applicable membranes for use in membrane contactors for flue gas desulfurization are proposed in this paper. The mass transfer mechanism for SO2 diffusion through gas filled pores is explored by simple measurements in order to identify suitable membrane structures for use in contactors for flue gas cleaning. It is attempted to correlate the experimentally determined membrane mass transfer coefficient to intrinsic physical properties of the membrane by applying theoretical and empirical correlations for the porosity-tortuosity relationship of the porous structure. Thereby limiting fluxes can be predicted with good accuracy from data quoted in the manufactures catalogue. 相似文献
56.
S C Bhatia S N Revankar K J Doshi N D Desai E D Bharucha C G Sahajwalla 《Journal of chromatography. A》1988,434(1):296-302
57.
The instability in a horizontal layer of a partially ionized self gravitating plasma has been studied to include simultaneously the effects of Hall currents, viscosity and finiteness of Larmor radius (FLR). The ambient magnetic field is assumed to be uniform and vertical. Proper solutions have been obtained through the variational methods for a semi-infinite plasma in which the density has an exponential gradient along the vertical. The dispersion relation obtained has been solved numerically and it is found that the growth rate of unstable perturbations decreases with the effects of viscosity, neutral gas friction and FLR. The influence of effects of viscosity, neutral gas friction and FLR are consequently stabilizing. It is found that the Hall currents have a destabilizing influence as the growth rate is found to increase with this effect. 相似文献
58.
We consider the time evolution of a class of stochastic systems of finite size with polynomial nearest neighbor transition rates. We obtain analytical expressions for the first passage time (FPT) and its moments. We show that the mean FPT, averaged over a uniform initial distribution, shows a simple asymptotoc behavior with the system size and the parameters of the transition rates. 相似文献
59.
T. Aziz S. Ahmad H. Ahrar S. B. Beri V. S. Bhatia S. N. Ganguli S. K. Gupta V. K. Gupta A. Gurtu G. L. Kaul Manjit Kaur A. R. Khan S. Kumar P. K. Malhotra L. K. Mangotra I. S. Mittra Y. Prakash N. K. Rao M. Shafi M. Shyam G. Singh S. K. Tuli Aligharh Collaboration Bombay Collaboration Chandigarh Collaboration Jammu Collaboration Varanasi Collaboration 《Zeitschrift fur Physik C Particles and Fields》1985,27(3):325-328
60.
We have studied the effect of rotation on the development of Rayleigh-Taylor instability of an incompressible, viscous, Hall, finitely conducting plasma of variable density. The solution is developed, through variational methods, for a semi-infinite plasma in which the density varies exponentially along the vertical. It is found that the system is unstable for all wave numbers when the effects of magnetic resistivity are included. The effects of coriolis forces and viscosity on the growth rate of the unstable system are found to be stabilizing while that of Hall currents is destabilizing. Finite conductivity affects the growth rate of the unstable mode differently for the smaller and larger values of the wave numbers, destabilizing for the waves of large wave length and stabilizing for waves of small wave length. 相似文献