Using Raman spectroscopy to monitor surface finish and roughness of components manufactured by selective laser sintering

Selective laser sintering (SLS) is an additive manufacturing process used to realise fully functional component manufacture. Numerous parameters are used in the process to control variables such as laser power, scan speed, laser spot size and overlap of scan vectors. All of these parameters can dramatically alter the sintering process and therefore final component properties. This paper presents how Raman spectroscopy intensity effects, caused by the surface roughness of the components produced, can be used to monitor the degree of sintering between particles in the SLS process. Copyright © 2010 John Wiley & Sons, Ltd.     

Perspective: pre-chemistry conformational changes in DNA polymerase mechanisms

In recent papers, there has been a lively exchange concerning theories for enzyme catalysis, especially the role of protein dynamics/pre-chemistry conformational changes in the catalytic cycle of enzymes. Of particular interest is the notion that substrate-induced conformational changes that assemble the polymerase active site prior to chemistry are required for DNA synthesis and impact fidelity (i.e., substrate specificity). High-resolution crystal structures of DNA polymerase β representing intermediates of substrate complexes prior to the chemical step are available. These structures indicate that conformational adjustments in both the protein and substrates must occur to achieve the requisite geometry of the reactive participants for catalysis. We discuss computational and kinetic methods to examine possible conformational change pathways that lead from the observed crystal structure intermediates to the final structures poised for chemistry. The results, as well as kinetic data from site-directed mutagenesis studies, are consistent with models requiring pre-chemistry conformational adjustments in order to achieve high fidelity DNA synthesis. Thus, substrate-induced conformational changes that assemble the polymerase active site prior to chemistry contribute to DNA synthesis even when they do not represent actual rate-determining steps for chemistry.     

Time-of-flight mass measurements for nuclear processes in neutron star crusts

We present results from time-of-flight nuclear mass measurements at the National Superconducting Cyclotron Laboratory that are relevant for neutron star crust models. The masses of 16 neutron-rich nuclei in the scandium-nickel range were determined simultaneously, with the masses of (61)V, (63)Cr, (66)Mn, and (74)Ni measured for the first time with mass excesses of -30.510(890) MeV, -35.280(650) MeV, -36.900(790) MeV, and -49.210(990) MeV, respectively. With these results the locations of the dominant electron capture heat sources in the outer crust of accreting neutron stars that exhibit super bursts are now experimentally constrained. We find the experimental Q value for the (66)Fe→(66)Mn electron capture to be 2.1 MeV (2.6σ) smaller than predicted, resulting in the transition occurring significantly closer to the neutron star surface.     

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Time-resolved photoconductivity of PbSe nanocrystal arrays

We report the sub-picosecond photoconductivity dynamics of chemically treated PbSe nanocrystal arrays utilizing time-resolved terahertz spectroscopy (TRTS). TRTS allows both the degree of interdot electronic coupling and the carrier dynamics to be extracted simultaneously. The following capping ligands bonded to the quantum dot surface were studied: hydrazine, ethylenediamine, butlyamine, and aniline. In addition, the arrays were treated with NaOH. We find that the treatments affect both the degree of electronic coupling and the carrier dynamics.     

Experimental limit on optical-photon coupling to light neutral scalar bosons

We report on the first results of a sensitive search for scalar coupling of photons to a light neutral boson in the mass range of approximately 1.0 meV (milli-electron volts) and coupling strength greater than 10(-6) GeV(-1) using optical photons. This was a photon regeneration experiment using the "light shining through a wall" technique in which laser light was passed through a strong magnetic field upstream of an optical beam dump; regenerated laser light was then searched for downstream of a second magnetic field region optically shielded from the former. Our results show no evidence for scalar coupling in this region of parameter space.     

Fast calculation of pulsed photoacoustic fields in fluids using k-space methods

Two related numerical models that calculate the time-dependent pressure field radiated by an arbitrary photoacoustic source in a fluid, such as that generated by the absorption of a short laser pulse, are presented. Frequency-wavenumber (k-space) implementations have been used to produce fast and accurate predictions. Model I calculates the field everywhere at any instant of time, and is useful for visualizing the three-dimensional evolution of the wave field. Model II calculates pressure time series for points on a straight line or plane and is therefore useful for simulating array measurements. By mapping the vertical wavenumber spectrum directly to frequency, this model can calculate time series up to 50 times faster than current numerical models of photoacoustic propagation. As the propagating and evanescent parts of the field are calculated separately, model II can be used to calculate far- and near-field radiation patterns. Also, it can readily be adapted to calculate the velocity potential and thus particle velocity and acoustic intensity vectors. Both models exploit the efficiency of the fast Fourier transform, and can include the frequency-dependent directional response of an acoustic detector straightforwardly. The models were verified by comparison with a known analytic solution and a slower, but well-understood, numerical model.     

A polydimethylsiloxane/glass capillary electrophoresis microchip for the analysis of biogenic amines using indirect fluorescence detection

A polydimethylsiloxane-glass capillary microchip is fabricated for the rapid analysis of a mixture of common biogenic amines using indirect fluorescence detection. Using a running buffer of phosphate and 2-propanol, and Rhodamine 110 as a background fluorophore, both co-ionic and counter-ionic systems are explored. Studies demonstrate the separation and analysis of cations using indirect fluorescence detection for the first time in a chip-based system. Resulting electrophoretic separations are achieved within a few tens of seconds with detection limits of approximately 6 microM. The reduced sample handling and rapid separations afforded by the coupling of indirect fluorescence detection with chip-based capillary electrophoresis provide a highly efficient method for the analysis and detection of molecules not possessing a chromophore or fluorophore. Furthermore, limits of detection are on a par with reported chip-based protocols that incorporate precolumn derivatisation with fluorescence detection. The current device circumvents lengthy sample preparation stages and therefore provides an attractive alternative technique for the analysis biogenic amines.