全文获取类型
收费全文 | 294篇 |
免费 | 2篇 |
国内免费 | 1篇 |
专业分类
化学 | 194篇 |
晶体学 | 27篇 |
力学 | 11篇 |
数学 | 38篇 |
物理学 | 27篇 |
出版年
2021年 | 2篇 |
2020年 | 3篇 |
2019年 | 3篇 |
2018年 | 3篇 |
2017年 | 3篇 |
2016年 | 3篇 |
2015年 | 3篇 |
2014年 | 2篇 |
2013年 | 16篇 |
2012年 | 11篇 |
2011年 | 24篇 |
2010年 | 9篇 |
2009年 | 5篇 |
2008年 | 18篇 |
2007年 | 9篇 |
2006年 | 17篇 |
2005年 | 17篇 |
2004年 | 10篇 |
2003年 | 17篇 |
2002年 | 16篇 |
2001年 | 10篇 |
2000年 | 9篇 |
1999年 | 3篇 |
1998年 | 6篇 |
1997年 | 6篇 |
1996年 | 5篇 |
1995年 | 4篇 |
1994年 | 2篇 |
1993年 | 3篇 |
1992年 | 6篇 |
1991年 | 3篇 |
1990年 | 5篇 |
1989年 | 5篇 |
1988年 | 4篇 |
1987年 | 4篇 |
1986年 | 3篇 |
1985年 | 3篇 |
1984年 | 3篇 |
1983年 | 3篇 |
1980年 | 2篇 |
1979年 | 2篇 |
1978年 | 3篇 |
1977年 | 3篇 |
1975年 | 1篇 |
1974年 | 1篇 |
1973年 | 1篇 |
1969年 | 1篇 |
1968年 | 1篇 |
1962年 | 1篇 |
1961年 | 1篇 |
排序方式: 共有297条查询结果,搜索用时 15 毫秒
61.
Prisecaru A Devereux M Barron N McCann M Colleran J Casey A McKee V Kellett A 《Chemical communications (Cambridge, England)》2012,48(55):6906-6908
The di-copper(II) cation, [Cu(2)(μ-terephthalate)(1,10-phen)(4)](2+), is a powerful, non-sequence-specific, minor-groove oxidizer of duplex DNA which, unlike copper(II) bis-1,10-phenanthroline chloride, operates independently of exogenous reagents. The agent displays excellent in vitro cytoxicity towards cisplatin-resistant ovarian cancer cells, producing intracellular reactive oxygen species upon nano-molar exposure. 相似文献
62.
DNA barcodes are short, unique ssDNA primers that "mark" individual biomolecules. To gain better understanding of biophysical parameters constraining primer-dimer formation between primers that incorporate barcode sequences, we have developed a capillary electrophoresis method that utilizes drag-tag-DNA conjugates to quantify dimerization risk between primer-barcode pairs. Results obtained with this unique free-solution conjugate electrophoresis approach are useful as quantitatively precise input data to parameterize computation models of dimerization risk. A set of fluorescently labeled, model primer-barcode conjugates were designed with complementary regions of differing lengths to quantify heterodimerization as a function of temperature. Primer-dimer cases comprised two 30-mer primers, one of which was covalently conjugated to a lab-made, chemically synthesized poly-N-methoxyethylglycine drag-tag, which reduced electrophoretic mobility of ssDNA to distinguish it from ds primer-dimers. The drag-tags also provided a shift in mobility for the dsDNA species, which allowed us to quantitate primer-dimer formation. In the experimental studies, pairs of oligonucleotide primer barcodes with fully or partially complementary sequences were annealed, and then separated by free-solution conjugate CE at different temperatures, to assess effects on primer-dimer formation. When less than 30 out of 30 base-pairs were bonded, dimerization was inversely correlated to temperature. Dimerization occurred when more than 15 consecutive base-pairs formed, yet non-consecutive base-pairs did not create stable dimers even when 20 out of 30 possible base-pairs bonded. The use of free-solution electrophoresis in combination with a peptoid drag-tag and different fluorophores enabled precise separation of short DNA fragments to establish a new mobility shift assay for detection of primer-dimer formation. 相似文献
63.
Melanie Egli Alicia Hartmann Helena Rapp Wright Keng Tiong Ng Frdric B. Piel Leon P. Barron 《Molecules (Basel, Switzerland)》2021,26(18)
The rapid source identification and environmental risk assessment (ERA) of hundreds of chemicals of emerging concern (CECs) in river water represent a significant analytical challenge. Herein, a potential solution involving a rapid direct-injection liquid chromatography–tandem mass spectrometry method for the quantitative determination of 102 CECs (151 qualitatively) in river water is presented and applied across six rivers in Germany and Switzerland at high spatial resolution. The method required an injection volume of only 10 µL of filtered sample, with a runtime of 5.5 min including re-equilibration with >10 datapoints per peak per transition (mostly 2 per compound), and 36 stable isotope-labelled standards. Performance was excellent from the low ng/L to µg/L concentration level, with 260 injections possible in any 24 h period. The method was applied in three separate campaigns focusing on the ERA of rivers impacted by wastewater effluent discharges (1 urban area in the Basel city region with 4 rivers, as well as 1 semi-rural and 1 rural area, each focusing on 1 river). Between 25 and 40 compounds were quantified directly in each campaign, and in all cases small tributary rivers showed higher CEC concentrations (e.g., up to ~4000 ng/L in total in the R. Schwarzach, Bavaria, Germany). The source of selected CECs could also be identified and differentiated from other sources at pre- and post- wastewater treatment plant effluent discharge points, as well as the effect of dilution downstream, which occurred over very short distances in all cases. Lastly, ERA for 41 CECs was performed at specific impacted sites, with risk quotients (RQs) at 1 or more sites estimated as high risk (RQ > 10) for 1 pharmaceutical (diclofenac), medium risk (RQ of 1–10) for 3 CECs (carbamazepine, venlafaxine, and sulfamethoxazole), and low risk (RQ = 0.1–1.0) for 7 CECs (i.e., RQ > 0.1 for 11 CECs in total). The application of high-throughput methods like this could enable a better understanding of the risks of CECs, especially in low flow/volume tributary rivers at scale and with high resolution. 相似文献
64.
Three kinds of grid system based on C-type grid are examined in order to reveal their relative flow characteristics of the turbomachinery cascade, especially near the trailing edge and wake. Here, a semi-conservative interpolation technique to treat the discontinuous boundary condition along the periodic boundary is proposed and is applied on the patched-type grid structure. Computational results are presented to see the influence of trailing-edge grid structure on the Navier-Stokes solutions for the high-turning transonic turbine cascade. 相似文献
65.
66.
67.
68.
Andrew R. Barron Geoffrey Wilkinson Majid Motevalli Michael B. Hursthouse 《Polyhedron》1985,4(6):1131-1134
The reaction of Rh2(O2CMe)4 with the sodium salt of 3,5-dimethylpyrazole (3,5-Me2pzH) in acetonitrile gives Rh2(3,5-Me2pz)4 · 2MeCN. This yellow diamagnetic compound on heating gives Rh(3,5-Me2pz)4 which in turn forms adducts with different unidentate ligands, L, to give Rh2(3,5-Me2pz)4 · 2L. The binuclear tetra bridged structure has been established for the acetonitrile complex by X-ray diffraction. The Rh-Rh distance is 2.353(3) Å and the Rh-N (acetonitrile) distance is 2.202(5) Å. Some unsubstituted pyrazolates have been made. 相似文献
69.
We prove uniqueness for extended real-valued lower semicontinuous viscosity solutions of the Bellman equation forL
-control problems. This result is then used to prove uniqueness for lsc solutions of Hamilton-Jacobi equations of the form –u
t
+H(t, x, u, –Du)=0, whereH(t, x, r, p) is convex inp. The remaining assumptions onH in the variablesr andp extend the currently known results.Supported in part by Grant DMS-9300805 from the National Science Foundation. 相似文献
70.