Flow injection analysis of sulfite ion with a potentiometric titanium phosphate-epoxy based membrane sensor

A novel pH sensor suitable for use in both aqueous and non-aqueous mediums is reported. The sensor is derived from polymer modified electrode obtained from electrochemical polymerisation of aniline in dry acetonitrile containing 0.5 M tetraphenyl borate at 2.0 V versus Ag/AgCl. The light yellow colour polymer modified electrode obtained under the present experimental condition has been characterised by scanning electron microscopy (SEM). The pH sensing of polymer modified electrode in both aqueous and non-aqueous mediums is examined and reported. As the typical examples, we used weak acid (acetic acid) and weak base (ammonium hydroxide) as analytes. The acetic acid is analysed in both aqueous and dry acetonitrile whereas ammonium hydroxide is analysed only in aqueous medium. The analysis in aqueous medium is conducted in 1 mM Tris-HCl buffer pH 7.0 and also in 0.1 M KCl. The slope of pH sensing is calculated from the data recorded in typical buffers and found to be approximately 86 mV per pH. The application of polymer modified electrode for the construction of urea biosensor is described based on immobilised urease within poly vinyl alcohol (PVA) matrix and also within organically modified sol-gel glass on the surface of polymer-modified electrode. The new urea sensor has shown maximum response of 160 mV at 25 degrees C with a lowest detection limit of 20 muM. The performance of new pH sensor and urea sensor has been studied and reported in this communication.     

Determination of lawsone in henna powders by high performance thin layer chromatography

The lawsone content has been evaluated quantitatively in eight commercial henna powders and two collected henna leaves. The phenolic, chloroform-soluble fraction of the majority of the examined samples showed the presence of lawsone and two other pigments. Here we aimed to optimize high performance thin layer chromatography for the determination of lawsone. Upon using the optimized method the examined samples showed considerable variation in lawsone concentration ranging from 0.004 up to 0.608 wt%, indicating that some samples were almost devoid of lawsone. Some of these products were subjected to preliminary in vivo toxicity studies.     

GC-MS/MS Quantification of EGFR Inhibitors, β-Sitosterol,Betulinic Acid, (+) Eriodictyol, (+) Epipinoresinol,and Secoisolariciresinol,in Crude Extract and Ethyl Acetate Fraction of Thonningia sanguinea

Medicinal plants are widely used in folk medicine to treat various diseases. Thonningia sanguinea Vahl is widespread in African traditional medicine, and exhibits antioxidant, antibacterial, antiviral, and anticancer activities. T. sanguinea is a source of phytomedicinal agents that have previously been isolated and structurally elucidated. Herein, gas chromatography combined with tandem mass spectrometry (GC-MS/MS) was used to quantify epipinoresinol, β-sitosterol, eriodictyol, betulinic acid, and secoisolariciresinol contents in the methanolic crude extract and its ethyl acetate fraction for the first time. The ethyl acetate fraction was rich in epipinoresinol, eriodictyol, and secoisolariciresinol at concentrations of 2.3, 3.9, and 2.4 mg/g of dry extract, respectively. The binding interactions of these compounds with the epidermal growth factor receptor (EGFR) were computed using a molecular docking study. The results revealed that the highest binding affinities for the EGFR signaling pathway were attributed to eriodictyol and secoisolariciresinol, with good binding energies of −19.93 and −16.63 Kcal/mol, respectively. These compounds formed good interactions with the key amino acid Met 769 as the co-crystallized ligand. So, the ethyl acetate fraction of T. sanguinea is a promising adjuvant therapy in cancer treatments.     

Synthesis,Spectroscopic, and Antimicrobial Activity Studies of Novel 10-Substituted Camptothecin Phosphorothioate Analogs

Abstract

A series of title compounds 2 and 3 were efficiently synthesized via the condensation of 10–hydroxycamptothecin with various symmetric (O,O′-monoaryl)-thiophosphoryl chlorides and asymmetric (O-ethyl-O′-aryl)-thiophosphoryl chlorides in sodium hydroxide powder and acetonitrile system. The structures of title compounds 2 and 3 were confirmed by elemental analysis, IR, ¹H NMR, ¹³C NMR, ³¹P[¹H] NMR,and mass spectral data. These symmetric [(O,O′-monoaryl)-thiophosphoryl)]-(20S)-camptothecin (2a–f) and asymmetric [(O-ethyl-O′-aryl)-thiophosphoryl)]-(20S)-camptothecin (3a–f) compounds were also tested for their in vitro antimicrobial activities against some bacterial strains, namely, S. aureus, B. Simplex, E. acetylicum, E. coli, P. aeruginosa, S. flexenari, S. aureus, S. typhi, and some fungal strains Aspergillus niger, Aspergillus flavus (molds), S. cerevisiae, C. albicans, T. longifucus, A. flavus, M. canis, F. solani, and C. glaberata (yeasts).

Supplemental materials are available for this article. Go to the publisher's online edition of Phosphorus, Sulfur, and Silicon and the related elements to view the free supplemental file.     

Entropy and renormalized solutions for nonlinear elliptic problem involving variable exponent and measure data

We give an existence result of entropy and renormalized solutions for strongly nonlinear elliptic equations in the framework of Sobolev spaces with variable exponents of the type:-div(a(x, u, u) + φ(u)) + g(x, u, u) = μ, where the right-hand side belongs to L1(Ω) + W-1,p(x)(Ω),-div(a(x, u, u)) is a Leray–Lions oper- ator defined from W-1,p(x)(Ω) into its dual and φ∈ C0(R, RN). The function g(x, u, u) is a non linear lower order term with natural growth with respect to |u| satisfying the sign condition, that is,g(x, u, u)u ≥ 0.     

Gagliardo-Nirenberg inequalities on manifolds

We prove Gagliardo-Nirenberg inequalities on some classes of manifolds, Lie groups and graphs.     

Effect of PVA surrounding medium on ZnSe nanoparticles: size, optical, and electrical properties

Polyvinyl alcohol (PVA) matrix was used to confine the particle size of ZnSe nanocrystallites as well as the variation of zinc (Zn) to selenium (Se) ion ratio which showed a remarkable decrease on the particle size as this ratio increased. The particle size decrease was monitored from the UV-vis absorption measurement as well as photoluminescence which suffered a blue shift with particle size decrease. The particle size was characterized with the aid of X-ray diffraction (XRD). The Raman spectra showed that, as the particle size increases, the peak position of the line centers (LO) mode were found to be red shifted from 239 to 234 cm(-1), accompanied by an increase in the full-width at half-maximum (FWHM). The electrical measurements and FT-IR spectra (overtone and normal) band vibration were used to study the effect of ZnSe NPs size on the PVA matrix.     

Riemann surfaces defined over the reals

The known (explicit) examples of Riemann surfaces not definable over their field of moduli are those with that field being a subfield of the reals but which cannot be defined over the reals. In this paper we provide explicit families of Riemann surfaces which are definable over the reals but cannot be defined over the field of moduli.