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131.
Oscar B. Torres Rashmi Jalah Kenner C. Rice Fuying Li Joshua F. G. Antoline Malliga R. Iyer Arthur E. Jacobson Mohamed Nazim Boutaghou Carl R. Alving Gary R. Matyas 《Analytical and bioanalytical chemistry》2014,406(24):5927-5937
A potential new treatment for drug addiction is immunization with vaccines that induce antibodies that can abrogate the addictive effects of the drug of abuse. One of the challenges in the development of a vaccine against drugs of abuse is the availability of an optimum procedure that gives reproducible and high yielding hapten-protein conjugates. In this study, a heroin/morphine surrogate hapten (MorHap) was coupled to bovine serum albumin (BSA) using maleimide-thiol chemistry. MorHap-BSA conjugates with 3, 5, 10, 15, 22, 28, and 34 haptens were obtained using different linker and hapten ratios. Using this optimized procedure, MorHap-BSA conjugates were synthesized with highly reproducible results and in high yields. The number of haptens attached to BSA was compared by 2,4,6-trinitrobenzenesulfonic acid (TNBS) assay, modified Ellman’s test and matrix assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). Among the three methods, MALDI-TOF MS discriminated subtle differences in hapten density. The effect of hapten density on enzyme-linked immunosorbent assay (ELISA) performance was evaluated with seven MorHap-BSA conjugates of varying hapten densities, which were used as coating antigens. The highest antibody binding was obtained with MorHap-BSA conjugates containing 3–5 haptens. This is the first report that rigorously analyzes, optimizes and characterizes the conjugation of haptens to proteins that can be used for vaccines against drugs of abuse. The effect of hapten density on the ELISA detection of antibodies against haptens demonstrates the importance of careful characterization of the hapten density by the analytical techniques described. 相似文献
132.
do Nascimento Ticiano Gomes Borges Arthur Luy Tavares Ferreira de Almeida Lara Mendes Ribeiro Êurica Adélia Nogueira Silva Fernanda Geny Calheiros da Costa Silva Valdemir do Nascimento Prata Ana Paula Basílio-Júnior Irinaldo Diniz Goulart Marília Oliveira Fonseca Morilla Demetrius Peireira de Freitas Johnnatan Duarte Aragão Cícero Flávio Soares 《Journal of Thermal Analysis and Calorimetry》2022,147(21):11949-11963
Journal of Thermal Analysis and Calorimetry - This work prepared and characterized microcapsule of Uncaria tomentosa (UT) in order to standardize a spray-dryer Uncaria tomentosa extract. The UT... 相似文献
133.
Yulia Shulpekova Vladimir Nechaev Svetlana Kardasheva Alla Sedova Anastasia Kurbatova Elena Bueverova Arthur Kopylov Kristina Malsagova Jabulani Clement Dlamini Vladimir Ivashkin 《Molecules (Basel, Switzerland)》2021,26(12)
Folates have a pterine core structure and high metabolic activity due to their ability to accept electrons and react with O-, S-, N-, C-bounds. Folates play a role as cofactors in essential one-carbon pathways donating methyl-groups to choline phospholipids, creatine, epinephrine, DNA. Compounds similar to folates are ubiquitous and have been found in different animals, plants, and microorganisms. Folates enter the body from the diet and are also synthesized by intestinal bacteria with consequent adsorption from the colon. Three types of folate and antifolate cellular transporters have been found, differing in tissue localization, substrate affinity, type of transferring, and optimal pH for function. Laboratory criteria of folate deficiency are accepted by WHO. Severe folate deficiencies, manifesting in early life, are seen in hereditary folate malabsorption and cerebral folate deficiency. Acquired folate deficiency is quite common and is associated with poor diet and malabsorption, alcohol consumption, obesity, and kidney failure. Given the observational data that folates have a protective effect against neural tube defects, ischemic events, and cancer, food folic acid fortification was introduced in many countries. However, high physiological folate concentrations and folate overload may increase the risk of impaired brain development in embryogenesis and possess a growth advantage for precancerous altered cells. 相似文献
134.
Ast CR Henk J Ernst A Moreschini L Falub MC Pacilé D Bruno P Kern K Grioni M 《Physical review letters》2007,98(18):186807
The long-range ordered surface alloy Bi/Ag(111) is found to exhibit a giant spin splitting of its surface electronic structure due to spin-orbit coupling, as is determined by angle-resolved photoelectron spectroscopy. First-principles electronic structure calculations fully confirm the experimental findings. The effect is brought about by a strong in-plane gradient of the crystal potential in the surface layer, in interplay with the structural asymmetry due to the surface-potential barrier. As a result, the spin polarization of the surface states is considerably rotated out of the surface plane. 相似文献
135.
Stocker Mariele K. Sanson Murilo L. Bernardes Arthur A. Netto Antonio Marchi Brambilla Rodrigo 《Journal of Sol-Gel Science and Technology》2021,98(3):568-579
Journal of Sol-Gel Science and Technology - In this work, silica capsules containing bromothymol blue (BTB) were synthesized by a modified Stöber sol–gel method. These silica capsules... 相似文献
136.
Xianwen Lou Christianus M. A. Leenders Arthur H. A. M. van Onzen Ralf A. A. Bovee Joost L. J. van Dongen Jef A. J. M. Vekemans E. W. Meijer 《Journal of the American Society for Mass Spectrometry》2014,25(2):297-300
Tetrahydrofuran (THF) is one of the most frequently used solvents in the MALDI TOF MS analysis of synthetic compounds. However, it should be used with caution because a trace amount of 4-hydroxybutanal (HBA) might be generated and accumulated in THF during storage. Since only a tiny amount of analytes is required in MALDI MS measurements, a trace amount of HBA might have a significant effect on the MS results. It was found that HBA will quickly react with primary and secondary amino compounds, leading to false results about the sample composition with an extra series of ions with additional mass of 70 Da in between. The formation of HBA can be inhibited by butylated hydroxytoluene (BHT) antioxidant. Therefore, when THF is required as the solvent for sample preparation, it is strongly recommended to use a BHT-stabilized one, at least for the analysis of compounds with amino groups. Figure
? 相似文献
137.
David L. Mobley Shuai Liu Nathan M. Lim Karisa L. Wymer Alexander L. Perryman Stefano Forli Nanjie Deng Justin Su Kim Branson Arthur J. Olson 《Journal of computer-aided molecular design》2014,28(4):327-345
Here, we give an overview of the protein-ligand binding portion of the Statistical Assessment of Modeling of Proteins and Ligands 4 (SAMPL4) challenge, which focused on predicting binding of HIV integrase inhibitors in the catalytic core domain. The challenge encompassed three components—a small “virtual screening” challenge, a binding mode prediction component, and a small affinity prediction component. Here, we give summary results and statistics concerning the performance of all submissions at each of these challenges. Virtual screening was particularly challenging here in part because, in contrast to more typical virtual screening test sets, the inactive compounds were tested because they were thought to be likely binders, so only the very top predictions performed significantly better than random. Pose prediction was also quite challenging, in part because inhibitors in the set bind to three different sites, so even identifying the correct binding site was challenging. Still, the best methods managed low root mean squared deviation predictions in many cases. Here, we give an overview of results, highlight some features of methods which worked particularly well, and refer the interested reader to papers in this issue which describe specific submissions for additional details. 相似文献
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