1,1,3,3-Tetramethylguanidine-gallane, (Me2N)2CN(H).GaH3: an unusually strongly bound gallane adduct

The novel adduct 1,1,3,3-tetramethylguanidine-gallane, (Me2N)2CN(H).GaH3, has been prepared by the reaction of [(Me2N)2CNH2]+Cl- with LiGaH4 in Et2O solution. Its spectroscopic properties indicate a monomeric species with an unusually strong coordinate link between the imido function and GaH3, an inference confirmed by the crystal structure at 150 K which also reveals significant secondary interactions through non-classical N-H...H-Ga bridges. Despite the intrinsic strength of the Ga-N bond, however, vaporisation at ca. 310 K occurs with partial dissociation, and decomposition via more than one pathway proceeds at temperatures >330-350 K to give a variety of products, including the free base, Me2NH, H2, and a novel gallium-nitrogen compound composed of a Ga4N4 cubane-like core bridged on three edges by -N{C(NMe2)2}GaH2- units.     

Basicity of a stable carbene, 1,3-di-tert-butylimidazol-2-ylidene,in THF

The basicity of 1,3-di-tert-butylimidazol-2-ylidene (1) was measured in THF against three hydrocarbon indicators. Both ion pairs and free ions were found and the corresponding equilibrium constants were measured. Homoconjugation was not found in either THF or DMSO. The carbene is effectively more basic in DMSO by several pK units, probably because of hydrogen bonding of 1-H(+) to DMSO. Model ab initio computations are consistent with these results.     

The stereospecificity at the iron center of the decarbonylation of an ironacetyl complex

The dinuclear complex [(h⁵-1-CH₃-3-C₆H₅C₅H₃)Fe(CO)₂]₂ was synthesized by reaction of Fe₂(CO)9 with 1-methyl-3-phenylcyclopentadiene; it was converted to (h⁵-1-CH₃-3-C₆H₅C₅H₃)Fe(CO)₂CH₃ by reduction with sodium amalgam and addition of CH₃l, and thence to (h⁵-1-CH₃-3-C₆H₅C₅H₃)Fe(CO)[P(C₆H₅)₃] (COCH₃) (I) by reaction with P(C₆H₅)₃. The acetyl I was separated into two diastereomerically related pairs of enantiomers. Ia and Ib, by a combination of column chromatography on alumina and crystallization from benzene/pentane. The photochemical decarbonylation of Ia and Ib in benzene or THF solution was examined by ¹H NMR spectroscopy. This reaction proceeds with high stereospecificity (>84% retention or inversion) at the iron center to yield (h⁵-1-CH₃-3-C₆H₈C₅H₃)Fe(CO)[P(C₆H₅)₃]CH₃(II), enriched in the diastereomerically related pairs of enantiomers, IIa and IIb, respectively. Since IIa and IIb epimerize under the photolytic conditions of decarbonylation, the actual stereospecificity of the conversion of I to II is higher than 84%, and likely 100%. This is supported by the data from kinetic studies of the decarbonylation of I and the epimerization of II, carried out under identical photolytic conditions. The implications of the foregoing results to the mechanism of the decarbonylation are considered. Also described herein is the synthesis of other complexes with two asymmetric centers of the general formula (h⁵-cyclopentadienyl)Fe(CO)(L)(COR) and (h⁵-cyclopentadienyl)Fe(CO)(L)R that contain either an unsymmetrically substituted h⁵-cyclopentadienyl ring or a chiral tertiary phosphine.     

Mechanism of the forbidden [3s,5s]-sigmatropic shift: orbital symmetry influences stepwise mechanisms involving diradical intermediates

The mechanisms of [3s,5s]-sigmatropic shifts of octa-1,3,7-triene and 7-methylenenona-1,3,8-triene have been elaborated using B3LYP and BPW91 density functional theory and CASPT2 methods. These orbital symmetry forbidden rearrangements are stepwise, involving diradical intermediates. A comparison with several [3,3]-sigmatropic shifts of substituted hexadienes and of [5,5]-sigmatropic shifts that are allowed, but nevertheless follow stepwise paths, shows that the activation barrier for the disallowed [3,5] shift is significantly larger than that for the stepwise reactions that are orbital symmetry allowed. Cyclic diradicals that have an aromatic circuit of electrons including the two radical centers and conjugated pi or sigma bonds are stabilized as compared to cyclic diradicals with an antiaromatic circuit of electrons. This applies to the transition states leading to and from the diradicals and influences the activation energies of stepwise sigmatropic shifts. The magnitudes of these effects are small but will have a significant influence on the rates of competing processes. This series of calculations has been used to assess the relative capabilities of the two functionals. We find that BPW91 underestimates the endothermicity of diradical formation and the barrier to diradical formation whereas B3LYP overestimates these quantities.     

Asymptotics for Euclidean minimal spanning trees on random points

Summary Asymptotic results for the Euclidean minimal spanning tree onn random vertices inR ^d can be obtained from consideration of a limiting infinite forest whose vertices form a Poisson process in allR ^d. In particular we prove a conjecture of Robert Bland: the sum of thed'th powers of the edge-lengths of the minimal spanning tree of a random sample ofn points from the uniform distribution in the unit cube ofR ^d tends to a constant asn.Whether the limit forest is in fact a single tree is a hard open problem, relating to continuum percolation.Research supported by N.S.F. Grants MCS87-11426 and MCS 90-01710Research supported in part by N.S.F. Grant DMS88-12868, A.F.O.S.R. Grant 89-0301, ARO Grant DAAL03-89-G-0092 and NSA Grant MDA-904-H-2034     

Enumeration of total yeasts and molds in foods by the SimPlate Yeast and Mold-Color Indicator method and conventional culture methods: collaborative study

The relative effectiveness of the SimPlate Yeast and Mold-Color Indicator method (Y&M-CI) was compared to the U.S. Food and Drug Administration's (FDA) Bacteriological Analytical Manual (BAM) method and the proposed International Organization for Standardization (ISO) method, ISO/CD 21527, for enumerating yeasts and molds in foods. Test portions were prepared and incubated according to the conditions stated in both the BAM and ISO methods. Six food types were analyzed: frozen corn dogs, nut meats, frozen fruits, cake mix, cereal, and fresh cheese. Nut meats, frozen fruits, and fresh cheese were naturally contaminated. All other foods were artificially contaminated with either a yeast or mold. Seventeen laboratories throughout North America and Europe participated in the study. Three method comparisons were conducted. In general, there was <0.3 mean log count difference in recovery between the SimPlate method and the 2 corresponding reference methods. Moreover, mean log counts between the 2 reference methods were also very similar. The repeatability (Sr) and reproducibility (SR) standard deviations were comparable between the 3 method comparisons. These results indicate that the BAM method and the SimPlate method are equivalent for enumerating yeast and mold populations in foods. Similarly, the SimPlate method is comparable to the proposed ISO method when test portions are prepared and incubated as defined in the proposed ISO method.     

Redox,transmetalation, and stacking properties of tetrathiafulvalene-2,3,6,7-tetrathiolate bridged tin,nickel, and palladium compounds

Here we report that capping the molecule TTFtt (TTFtt = tetrathiafulvalene-2,3,6,7-tetrathiolate) with dialkyl tin groups enables the isolation of a stable series of redox congeners and facile transmetalation to Ni and Pd. TTFtt has been proposed as an attractive building block for molecular materials for two decades as it combines the redox chemistry of TTF and dithiolene units. TTFttH₄, however, is inherently unstable and the incorporation of TTFtt units into complexes or materials typically proceeds through the in situ generation of the tetraanion TTFtt⁴⁻. Capping of TTFtt⁴⁻ with Bu₂Sn²⁺ units dramatically improves the stability of the TTFtt moiety and furthermore enables the isolation of a redox series where the TTF core carries the formal charges of 0, +1, and +2. All of these redox congeners show efficient and clean transmetalation to Ni and Pd resulting in an analogous series of bimetallic complexes capped by 1,2-bis(diphenylphosphino)ethane (dppe) ligands. Furthermore, by using the same transmetalation method, we synthesized analogous palladium complexes capped by 1,1′-bis(diphenylphosphino)ferrocene (dppf) which had been previously reported. All of these species have been thoroughly characterized through a systematic survey of chemical and electronic properties by techniques including cyclic voltammetry (CV), ultraviolet-visible-near infrared spectroscopy (UV-vis-NIR), electron paramagnetic resonance spectroscopy (EPR), nuclear magnetic resonance spectroscopy (NMR) and X-ray diffraction (XRD). These detailed synthetic and spectroscopic studies highlight important differences between the transmetalation strategy presented here and previously reported synthetic methods for the installation of TTFtt. In addition, the utility of this stabilization strategy can be illustrated by the observation of unusual TTF radical–radical packing in the solid state and dimerization in the solution state. Theoretical calculations based on variational 2-electron reduced density matrix methods have been used to investigate these unusual interactions and illustrate fundamentally different levels of covalency and overlap depending on the orientations of the TTF cores. Taken together, this work demonstrates that tin-capped TTFtt units are ideal reagents for the installation of redox-tunable TTFtt ligands enabling the generation of entirely new geometric and electronic structures.

Capping TTFtt enables facile transmetalation in three different oxidation states.     

Vibronic effects accelerate the intersystem crossing processes of the through-space charge transfer states in the triptycene bridged acridine–triazine donor–acceptor molecule TpAT-tFFO

Quantum chemical studies employing combined density functional and multireference configuration interaction methods suggest five excited electronic states to be involved in the prompt and delayed fluorescence emission of TpAT-tFFO. Three of them, a pair of singlet and triplet charge transfer (CT) states (S₁ and T₁) and a locally excited (LE) triplet state (T₃), can be associated with the (Me → N) conformer, the other two CT-type states (S₂ and T₂) form the lowest excited singlet and triplet states of the (Me → Ph) conformer. The two conformers, which differ in essence by the shearing angle of the face-to-face aligned donor and acceptor moieties, are easily interconverted in the electronic ground state whereas the reorganization energy is substantial in the excited singlet state, thus explaining the two experimentally observed time constants of prompt fluorescence emission. Forward and reverse intersystem crossing between the singlet and triplet CT states is mediated by vibronic spin–orbit interactions involving the LE T₃ state. Low-frequency vibrational modes altering the distance and alignment of the donor and acceptor π-systems tune the S₁ and T₃ states (likewise S₂ and T₃) into and out of resonance. The enhancement of intersystem crossing due to the interplay of vibronic and spin–orbit coupling is considered a general feature of organic through-space charge-transfer thermally activated delayed fluorescence emitters.

DFT/MRCI quantum chemical studies suggest five excited electronic states to be involved in the prompt and delayed fluorescence emission of TpAT-tFFO.     

The Extracts of Polygonum cuspidatum Root and Rhizome Block the Entry of SARS-CoV-2 Wild-Type and Omicron Pseudotyped Viruses via Inhibition of the S-Protein and 3CL Protease

COVID-19, resulting from infection by the SARS-CoV-2 virus, caused a contagious pandemic. Even with the current vaccines, there is still an urgent need to develop effective pharmacological treatments against this deadly disease. Here, we show that the water and ethanol extracts of the root and rhizome of Polygonum cuspidatum (Polygoni Cuspidati Rhizoma et Radix), a common Chinese herbal medicine, blocked the entry of wild-type and the omicron variant of the SARS-CoV-2 pseudotyped virus into fibroblasts or zebrafish larvae, with IC₅₀ values ranging from 0.015 to 0.04 mg/mL. The extracts were shown to inhibit various aspects of the pseudovirus entry, including the interaction between the spike protein (S-protein) and the angiotensin-converting enzyme II (ACE2) receptor, and the 3CL protease activity. Out of the chemical compounds tested in this report, gallic acid, a phytochemical in P. cuspidatum, was shown to have a significant anti-viral effect. Therefore, this might be responsible, at least in part, for the anti-viral efficacy of the herbal extract. Together, our data suggest that the extracts of P. cuspidatum inhibit the entry of wild-type and the omicron variant of SARS-CoV-2, and so they could be considered as potent treatments against COVID-19.