Direct discrimination of cell surface glycosylation signatures using a single pH-responsive boronic acid-functionalized polymer

Cell surface glycans serve fundamental roles in many biological processes, including cell–cell interaction, pathogen infection, and cancer metastasis. Cancer cell surface have alternative glycosylation to healthy cells, making these changes useful hallmarks of cancer. However, the diversity of glycan structures makes glycosylation profiling very challenging, with glycan ‘fingerprints’ providing an important tool for assessing cell state. In this work, we utilized the pH-responsive differential binding of boronic acid (BA) moieties with cell surface glycans to generate a high-content six-channel BA-based sensor array that uses a single polymer to distinguish mammalian cell types. This sensing platform provided efficient discrimination of cancer cells and readily discriminated between Chinese hamster ovary (CHO) glycomutants, providing evidence that discrimination is glycan-driven. The BA-functionalized polymer sensor array is readily scalable, providing access to new diagnostic and therapeutic strategies for cell surface glycosylation-associated diseases.

A high-content multichannel sensor array was developed to efficiently discriminate cell states. The binding of boronic acid with glycans is pH-responsive, hence utilizing three pH provides a sensitive multidimensional array from a single polymer.     

Ligand-stabilized metal nanoparticles in organic solvent

This critical review reports the fundamental behavior of metal nanoparticles in different organic solvents, i.e., metal organosol. An overview on metal organosol and then their smart synthetic approaches, characterization, and potential applications in the fields of catalysis and spectroscopy with special emphasis on SERS are embodied. Aspects of organosol fabrication, stabilization, morphology control, growth mechanisms, and physical properties as mono- and bimetallic nanoparticles are discussed. The article inspires the repetitive usage of metal nanoparticles as stable deliverable organic and molecular compounds.     

Stability of magnetohydrodynamic stratified shear flows

Summary The linear stability of a stratified shear flow of a perfectly conducting bounded fluid in the presence of a magnetic field aligned with the flow and buoyancy forces has been studied under Boussinesq approximation. A new upper bound has been obtained for the range of real and imaginary parts of the complex wave velocity for growing perturbations. The upper bound depends on minimum Richardson number, wave number, Alfvén velocity and basic flow velocity. H?iland's necessary criterion for instability of hydrodynamic stratified homogeneous shear flow is modified and its analog for nonhomogeneous magnetohydrodynamic cases is derived. Finally the upper bound for the growth rate ofKC _i and its variants, whereK is the wave number andC _i the imaginary part of complex wave velocity, is derived as the necessary condition of instability. All estimates remain valid even when the minimum richardson numberJ ₁, for some practical problems, exceeds 1/4 for growing perturbations. The authors of this paper have agreed to not receive the proofs for correction.     

Kinetics of oxidation of methanol and mono-deutero-methanol by chromium(VI) in perchloric acid medium

Kinetics of the oxidation of methanol and mono-deutero-methanol by Cr(VI) over a wide range of temperature (25–40°) have been studied in perchloric acid medium at constant ionic strength (μ = 1.0 M) adjusted with sodium perchlorate. Each reaction is first order with respect to the substrate and dichromate concentrations but the order with respect to [H⁺] is nearly 3 in each case. Both these reactions take place at almost the same rate under identical experimental conditions. The activation parameters of the reactions are not widely different and the values of ΔH3 and ΔS3 for the oxidation of methanol are 79.5 kJ mole ^-1 and - 38.1 J deg^-1 mole^-1 respectively, whereas the corresponding values for the deuterated compound are 83.8 kJ mole^-1 and -23.9 J deg^-1 mole^-1. The probable mechanism of the reactions is discussed.     

All-optical carry lookahead adder with the help of terahertz optical asymmetric demultiplexer

An all-optical model of carry lookahead adder (CLA) implemented with a semiconductor optical amplifier (SOA)-assisted Sagnac interferometer (TOAD) is presented. The model accounts for the SOA small signal gain, linewidth enhancement factor, the switching pulses energy and width and the Sagnac loop asymmetry. Adder is a very basic component in a central processing unit. The CLA is the highest speed adder nowadays. Theoritical model is presented and verified through numerical simulation. The method promises both higher processing speed and accuracy. The model can be enhanced the functionality in which carry lookahead adder is the basic building block.     

Femtosecond laser fabrication of high‐Q whispering gallery mode microresonators via two‐photon polymerization

Whispering gallery mode microresonators have been triggering considerable advances in science due to their ability to confine light within small dielectric volumes, which makes them suitable for a wide range of applications. Lithographic approaches have been the dominant technique for fabricating microresonators; however, they restrict the choice of materials due to their multistep processing nature. As an alternative, they report the direct laser fabrication of acrylic based hollow microcylinder resonators, via two‐photon polymerization, with good structural integrity and sidewall roughness of 1.5 nm, which make them promising candidates for photonic applications in the near‐infrared. Such polymeric microresonators exhibit finesse close to 10³ and a quality factor of 1 10⁵, a performance achieved without any additional processing step, which would restrict the choices of materials to be incorporated into the polymeric resonator. This advantage thereby broadens the widespread use of the polymeric microresonators, making them an excellent platform for lasing and nonlinear optics studies in the near‐infrared. © 2017 Wiley Periodicals, Inc. J. Polym. Sci., Part B: Polym. Phys. 2017 , 55, 569–574     

A 1D Cu(II) coordination polymer exhibiting ferromagnetic interactions and a mononuclear Cu(II) complex of substituted pyrazole carboxylic acids: Synthesis, characterization and crystal structure

Reaction of 2 equiv. amount of copper(II) nitrate hexahydrate with 1 equiv. of 5-methyl-1-pyridin-2-yl-1H-pyrazole-3-carboxylic acid (PyPzCA) in presence of triethyl amine base afforded a 1D coordination polymeric compound [Cu₂(PyPzCA)₂(H₂O)₃(NO₃)]NO₃·H₂O (1). Whereas, the same reaction when repeated with 1-(4,6-dimethyl-pyrimidin-2-yl)-5-methyl-1H-pyrazole-3-carboxylic acid (PymPzCA) instead of PyPzCA, a mononuclear compound [Cu(PymPzCA)]·2H₂O·NEt₃ (2) is formed. Both the complexes are crystallographically characterized. In 1, both the copper atoms (Cu1 and Cu2) have distorted square pyramidal geometry with N₂O₃ chromophore while, in 2, the central copper atom has a distorted trigonal bipyramidal geometry with N₄O chromophore. Complex 1, is a 1D coordination polymer where the metal centers being far apart and are involved in a weak ferromagnetic interaction which is quite unexpected.     

Positronium formation cross-sections in the ground and excited states ( levels) in an -He atom collision

The positronium formation cross-sections in the ground and excited n=2 levels have been studied in an -He atom collision in the framework of eikonal approximation. Both the differential and total formation cross-sections have been investigated in the intermediate- and high-energy regime. Present eikonal results are found to differ appreciably from the corresponding first Born values even at very high incident energies. The total cross-section results have been compared with available experiments due to different groups as well as with other existing theoretical results. Received: 20 July 1998 / Received in final form: 5 January 1999     

Unsteady MHD Flow on a Rotating Cone in a Rotating Fluid

Unsteady flow over an infinite permeable rotating cone in a rotating fluid in the presence of an applied magnetic field has been investigated. The unsteadiness is induced by the time-dependent angular velocity of the body, as well as that of the fluid. The partial differential equations governing the flow have been solved numerically by using an implicit finite-difference scheme in combination with the quasi-linearization technique. For large values of the magnetic parameter, analytical solutions have also been obtained for the steady-state case. It is observed that the magnetic field, surface velocity, and suction and injection strongly affect the local skin friction coefficients in the tangential and azimuthal directions. The local skin friction coefficients increase when the angular velocity of the fluid or body increases with time, but these decrease with decreasing angular velocity. The skin friction coefficients in the tangential and azimuthal directions vanish when the angular velocities of fluid and the body are equal but this does not imply separation. When the angular velocity of the fluid is greater than that of the body, the velocity profiles reach their asymptotic values at the edge of the boundary layer in an oscillatory manner, but the magnetic field or suction reduces or suppresses these oscillations.