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21.
Dr. Valerio Voliani Dr. María González‐Béjar Dr. Vicente Herranz‐Pérez M.Sc. Maria Duran‐Moreno Dr. Giovanni Signore Prof. Jose M. Garcia‐Verdugo Prof. Julia Pérez‐Prieto 《Chemistry (Weinheim an der Bergstrasse, Germany)》2013,19(40):13538-13546
A simple and straightforward method for the orthogonal functionalisation of upconverting NaYF4 nanocrystals (UCNCs)—doped withYb3+ and Er3+—based on N‐(3‐dimethylaminopropyl)‐N′‐ethylcarbodiimide/N‐hydroxysuccinimide (EDC/NHS) selective reactions between two dyes and two different reactive groups present at the periphery of the upconverting nanocrystals is reported. Organic‐soluble UCNCs of 10 and 50 nm in size are encapsulated efficiently in a 1:1 mixture of two commercial 3000 Da poly(ethylene glycol) derivatives with two different reactive groups (amino and carboxylic groups). The water‐dispersible UCNCs are non‐cytotoxic, stable in the physiological environment, and present free amine and carboxylic reactive groups on their periphery, allowing rapid, selective, and modular covalent conjugation to payloads through EDC/NHS reactions. PEG‐encapsulated UCNCs with and without covalent conjugation to payloads are characterised in vitro through spectroscopic, dynamic light scattering, and electron microscopy measurements. Living cell analyses coupled with TEM measurements confirm the uptake and low cytotoxicity of the coated UCNCs. They are linked covalently to two different dyes, internalised by living cells, and analysed by confocal microscopy. The related colocalisation measurements prove the reactivity of both amines and carboxylic acids on the periphery of the nanocrystals. This approach demonstrates that it is possible to produce water‐dispersible and cyto‐compatible dual‐functional UCNCs. 相似文献
22.
Dr. Sabina Rodríguez‐Hermida Dr. Min Ying Tsang Claudia Vignatti Dr. Kyriakos C. Stylianou Dr. Vincent Guillerm Dr. Javier Pérez‐Carvajal Prof. Francesc Teixidor Prof. Clara Viñas Dr. Duane Choquesillo‐Lazarte Dr. Cristóbal Verdugo‐Escamilla Dr. Inmaculada Peral Dr. Jordi Juanhuix Dr. Albert Verdaguer Dr. Inhar Imaz Prof. Daniel Maspoch Dr. José Giner Planas 《Angewandte Chemie (International ed. in English)》2016,55(52):16049-16053
Materials with surfaces that can be switched from high/superhydrophobicity to superhydrophilicity are useful for myriad applications. Herein, we report a metal–organic framework (MOF) assembled from ZnII ions, 1,4‐benzenedicarboxylate, and a hydrophobic carborane‐based linker. The MOF crystal‐surface can be switched between hydrophobic and superhydrophilic through a chemical treatment to remove some of the building blocks. 相似文献
23.
Sulfotransferases catalyze the transfer of a sulfuryl group from the eukaryotic sulfate donor 3'-phosphoadenosine 5'-phosphosulfate to an acceptor biomolecule. Sulfotransferases have been linked with several disease states, prompting our investigation of specific sulfotransferase inhibitors. Presented herein is the synthesis and evaluation of a bisubstrate analogue designed to inhibit estrogen sulfotransferase. The synthesis utilizes a novel, orthogonally protected 3'-phosphoadenosine 5'-phosphate (PAP) derivative allowing the selective functionalization of the 5'-phosphate with a sulfate acceptor mimic. Kinetic studies revealed significant inhibitory activity and provide guidance for improved inhibitor design. 相似文献