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An immobiline DryStrip application method enabling high-capacity two-dimensional gel electrophoresis 总被引:5,自引:0,他引:5
Sabounchi-Schütt F Aström J Olsson I Eklund A Grunewald J Bjellqvist B 《Electrophoresis》2000,21(17):3649-3656
In the field of proteomics the need to detect low-abundance cellular components, such as regulatory proteins, is of critical importance. Two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) is one of the most commonly used separation tools for these biological investigations. In this paper we report an alternative micropreparative 2-D PAGE sample application method, called the "paper bridge loading" method. This method makes it possible to apply a larger sample volume to commercially available immobilized pH gradient (IPG) strips. The Vh products required for focusing are only marginally longer than those used in analytical experiments. The method was compared to traditional cup loading and in-gel rehydration. With 18 cm long narrow-range Immobiline DryStrip pH 4.5-5.5, the "paper bridge" method allowed the application of 10 mg human plasma proteins compared to 3 mg with traditional loading methods. The corresponding figures using Escherichia coli sample was found to be 6 mg and less than 2 mg, respectively. The paper bridge method also showed the best results in terms of spot resolution and separation of high molecular weight proteins. 相似文献
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I. Gömöry 《Journal of Thermal Analysis and Calorimetry》1977,11(2):327-334
A survey and an analysis of the publications concerned in thermal analysis (TA) published in four renowned journals during the years 1965–1974 were performed. The published data were evaluated from three points of view: TA methods used for polymers, properties and phenomena studied by TA methods, types of polymers investigated by TA methods. From the statistical data were derived the future trends for the application of particular TA methods in polymer science. 相似文献
16.
LDPE synthesized under two-phase conditions is known to have a good balance of blown film properties. Good optical properties and high impact strength are characteristic for two-phase LDPE grades. In order to obtain systematic information, LDPE was synthesized at several different pressures with two different melt index levels. Blown film was extruded at different output rates and melt temperatures. The influence on haze and dart drop impact was studied. Two-phase conditions were found to improve haze and dart drop impact and the improvement was impressive, especially for the low melt index grades. By increasing the output rate, the dart drop values were further improved. The influence of melt temperature on dart drop impact goes through an optimum and overall haze decreases with increasing melt temperature. For films made from two-phase grades, dart drop impact decreases as internal haze increases. For corresponding internal haze values, the films made from single-phase grades have lower dart drop values and they are somewhat scattered. 相似文献
17.
Condensation of bis-(2-chloroethyl)phosphoramidic dichloride with 3′-amino-3′-deoxy-N,N-dimethyladenosine afforded the 2′,3′-cyclicphosphorodiamidate (III). By an improved synthesis, methyl 3-amino-3-deoxy-β-D-ribofuranoside was obtained as a model compound for conversion to the analogous 2,3-cyclicphosphorodiamidate (XII). Existence of the latter as two diastereomers due to phosphorus asymmetry was shown by nmr analysis, using comparison with the 5-(O-p-nitrobenzoate) (XIII) as a basis for assignments. 相似文献
18.
Abstract— This paper reports the present conclusions of an extended investigation on photo-sensitization of silver bromide. A general molecular packing structure for dye aggregates on the AgBr (111) surface is proposed. This structure, along with the observed spectral absorption displacements of small aggregates, is used to elucidate the phenomena of antisensitization and supersensitization. Supersensitization is seen as a partitioning of large dye aggregates into small aggregates by a deliberately added second component or by gaps between the aggregates. This partitioning isolates antisensitizing dye in a small fraction of the aggregates and minimizes its effect. The smallest aggregates are least likely to contain an antisensitizer, and show the highest quantum efficiency for photoconductivity and photographic action. Photoconductivity measurements establish that supersensitization occurs before the electron appears in the AgBr phase.
The question of direct electron injection vs. energy transfer as mechanisms for AgBr photosensitization is examined in terms of radiationless transfer to surface Ag2 S on AgBr. The absorption of surface Ag2 S is shown to be adequate for acceptance of Förster transfer from the dye, and surface Ag2 S is known to be photographically active. However, this mechanism is inefficient, and inadequate to account for observed high efficiency infrared sensitization. Direct electron injection is seen as the more probable mechanism for efficient dye sensitization of AgBr. 相似文献
The question of direct electron injection vs. energy transfer as mechanisms for AgBr photosensitization is examined in terms of radiationless transfer to surface Ag
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The photodegradation of 1,3-dimethyl-1-(2-(3-fluorobenzylthio)-1,3,4-thiadiazol-5-yl)urea as a thin film and in solution is described. The two photoproducts from thin-film photolysis were characterized by spectral and synthetic methods. The X-ray crystal structure of one of the photoproducts is also reported. The rearrangements were shown to involve an S-to-N benzyl migration, followed by a sulfur-oxygen substitution. 相似文献
20.
Pressurized sample preparation devices (High Pressure Asher, Pressurized Microwave Digestion system, compared with a PTFE decomposition vessel) were used to dissolve certified metal alloy samples (steel, copper) for ICP analysis. Based on the results of the analysis it was established that both up-to-date devices can be advantageously applied to quickly and quantitatively dissolve metal alloy samples. To dissolve the samples, two different kinds of acid mixtures (A: nitric and hydrochloric acid; B: nitric and hydrochloric and sulphuric and phosphoric acid) were used. The sample preparation is simpler and less time-consuming than the earlier commonly used methods, sample loss and degree of contamination are also reduced. Steel samples containing tungsten, titanium and niobium (less than 0.5%) can only be analyzed using a mixture of the four acids. By dissolving steel samples in the nitric and hydrochloric acid mixture, the concentration of their most common elements (Cr, Ni, Mn, V, Cu) as well as their S and P content can be determined. Copper alloy samples can be dissolved quickly by the pressurized microwave decomposition device using hydrochloric acid and diluted (1:1) nitric acid. 相似文献