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991.
Stefanescu R Born R Moise A Ernst B Przybylski M 《Journal of the American Society for Mass Spectrometry》2011,22(1):148-157
Recent studies suggest that the H1 subunit of the carbohydrate recognition domain (H1CRD) of the asialoglycoprotein receptor
is used as an entry site into hepatocytes by hepatitis A and B viruses and Marburg virus. Thus, molecules binding specifically
to the CRD might exert inhibition towards these diseases by blocking the virus entry site. We report here the identification
of the epitope structure of H1CRD to a monoclonal antibody by proteolytic epitope excision of the immune complex and high-resolution
MALDI-FTICR mass spectrometry. As a prerequisite of the epitope determination, the primary structure of the H1CRD antigen
was characterised by ESI-FTICR-MS of the intact protein and by LC-MS/MS of tryptic digest mixtures. Molecular mass determination
and proteolytic fragments provided the identification of two intramolecular disulfide bridges (seven Cys residues), and a
Cys-mercaptoethanol adduct formed by treatment with β-mercaptoethanol during protein extraction. The H1CRD antigen binds to
the monoclonal antibody in both native and Cys-alkylated form. For identification of the epitope, the antibody was immobilized
on N-hydroxysuccinimide (NHS)-activated Sepharose. Epitope excision and epitope extraction with trypsin and FTICR-MS of affinity-bound
peptides provided the identification of two specific epitope peptides (5–16) and (17–23) that showed high affinity to the
antibody. Affinity studies of the synthetic epitope peptides revealed independent binding of each peptide to the antibody. 相似文献
992.
Replicating cells undergo DNA synthesis in the highly regulated, S-phase of the cell cycle. Analogues of the pyrimidine deoxynucleoside thymidine may be inserted into replicating DNA, effectively tagging dividing cells allowing their characterisation. Tritiated thymidine, targeted using autoradiography was technically demanding and superseded by 5-bromo-2-deoxyuridine (BrdU) and related halogenated analogues, detected using antibodies. Their detection required the denaturation of DNA, often constraining the outcome of investigations. Despite these limitations BrdU alone has been used to target newly synthesised DNA in over 20,000 reviewed biomedical studies. A recent breakthrough in "tagging DNA synthesis" is the thymidine analogue 5-ethynyl-2'-deoxyuridine (EdU). The alkyne group in EdU is readily detected using a fluorescent azide probe and copper catalysis using 'Huisgen's reaction' (1,3-dipolar cycloaddition or 'click chemistry'). This rapid, two-step biolabelling approach allows the tagging and imaging of DNA within cells whilst preserving the structural and molecular integrity of the cells. The bio-orthogonal detection of EdU allows its application in more experimental assays than previously possible with other "unnatural bases". These include physiological, anatomical and molecular biological experimentation in multiple fields including, stem cell research, cancer biology, and parasitology. The full potential of EdU and related molecules in biomedical research remains to be explored. 相似文献
993.
Selective particle trapping using an oscillating microbubble 总被引:1,自引:0,他引:1
The ability to isolate and sort analytes within complex microfluidic volumes is essential to the success of lab-on-a-chip (LOC) devices. In this study, acoustically-excited oscillating bubbles are used to selectively trap particles, with the selectivity being a function of both particle size and density. The operating principle is based on the interplay between the strong microstreaming-induced drag force and the attractive secondary Bjerknes force. Depending upon the size of the bubble, and thus its resonant frequency, it is possible to cause one force to dominate over the other, resulting in either particle attraction or repulsion. A theoretical analysis reveals the extent of the contribution of each force for a given particle size; in close agreement with experimental findings. Density-based trapping is also demonstrated, highlighting that denser particles experience a larger secondary Bjerknes force resulting in their attraction. This study showcases the excellent applicability and versatility of using oscillating bubbles as a trapping and sorting mechanism within LOC devices. 相似文献
994.
Jarvis AG Whitwood AC Fairlamb IJ 《Dalton transactions (Cambridge, England : 2003)》2011,40(14):3695-3702
The synthesis and characterisation of a multidentate conformationally flexible ligand based on the dibenzylidene acetone core structure, dbathiophos (1), is described. Ligand 1 has a high affinity for cationic and neutral Cu(I) species. Three unique Cu(I) complexes (4-6) are reported showing that the ligand backbone of dbathiophos is hemilabile, and able to adopt different 1,4-dien-3-one conformational geometries around Cu(I). Complexes 4 and 6 both effectively catalyse the cyclopropanation of styrene with ethyl diazoacetate at low catalyst loadings (1 mol% Cu). 相似文献
995.
Urbaczek S Kolodzik A Fischer JR Lippert T Heuser S Groth I Schulz-Gasch T Rarey M 《Journal of chemical information and modeling》2011,51(12):3199-3207
In most cheminformatics workflows, chemical information is stored in files which provide the necessary data for subsequent calculations. The correct interpretation of the file formats is an important prerequisite to obtain meaningful results. Consistent reading of molecules from files, however, is not an easy task. Each file format implicitly represents an underlying chemical model, which has to be taken into consideration when the input data is processed. Additionally, many data sources contain invalid molecules. These have to be identified and either corrected or discarded. We present the chemical file format converter NAOMI, which provides efficient procedures for reliable handling of molecules from the common chemical file formats SDF, MOL2, and SMILES. These procedures are based on a consistent chemical model which has been designed for the appropriate representation of molecules relevant in the context of drug discovery. NAOMI's functionality is tested by round robin file IO exercises with public data sets, which we believe should become a standard test for every cheminformatics tool. 相似文献
996.
We use elliptic theory to prove the existence of steady two-dimensional periodic waterwaves of large amplitude in a flowwith
an arbitrary bounded but discontinuous vorticity. This is achieved by developing a local and global bifurcation construction
of weak solutions of the elliptic partial differential equations that are relevant to this hydrodynamical context. 相似文献
997.
Adrian Constantin Joachim Escher Hung-Chu Hsu 《Archive for Rational Mechanics and Analysis》2011,201(1):251-269
We study the pressure beneath a solitary water wave propagating in an irrotational flow at the free surface of water with
a flat bed. The investigation is divided into two parts. The first part concerns a rigorous nonlinear study of the governing
equations for water waves. We prove that the pressure in the fluid beneath a solitary wave strictly increases with depth and
strictly decreases horizontally away from the vertical line beneath the crest. The second part of the paper describes an experimental
study. Excellent agreement is found to exist between the theoretical predictions and the measurements. Our conclusions are
also supported by numerical simulations. 相似文献
998.
Saielli G Nicolaou KC Ortiz A Zhang H Bagno A 《Journal of the American Chemical Society》2011,133(15):6072-6077
We have employed density functional theory (DFT) protocols to calculate the NMR properties of the vannusals, a class of natural products whose structures have been the subject of recent investigations. The originally assigned structure of vannusal B was revised after a long synthetic journey which generated a series of closely related diastereomers. In this work we show how DFT calculations based on density functionals and basis sets designed for the prediction of NMR spectra (M06/pcS-2 level of theory) can be used to reproduce the observed parameters, thereby offering to the synthetic chemist a useful tool to discard or accept putative structures of unknown organic molecules. 相似文献
999.
Lonsdale R Oláh J Mulholland AJ Harvey JN 《Journal of the American Chemical Society》2011,133(39):15464-15474
The cytochrome P450 (CYP) enzymes are important in many areas, including pharmaceutical development. Subtle changes in the electronic structure of the active species, Compound I, have been postulated previously to account partly for the experimentally observed differences in reactivity between isoforms. Current predictive models of CYP metabolism typically assume an identical Compound I in all isoforms. Here we present a method to calculate the electronic structure and to estimate the Fe-O bond enthalpy of Compound I, and apply it to several human and bacterial CYP isoforms. Conformational flexibility is accounted for by sampling large numbers of structures from molecular dynamics simulations, which are subsequently optimized with density functional theory (B3LYP) based quantum mechanics/molecular mechanics. The observed differences in Compound I between human isoforms are small: They are generally smaller than the spread of values obtained for the same isoform starting from different initial structures. Hence, it is unlikely that the variation in activity between human isoforms is due to differences in the electronic structure of Compound I. A larger difference in electronic structure is observed between the human isoforms and P450(cam) and may be explained by the slightly different hydrogen-bonding environment surrounding the cysteinyl sulfur. The presence of substrate in the active site of all isoforms studied appears to cause a slight decrease in the Fe-O bond enthalpy, apparently due to displacement of water out of the active site, suggesting that Compound I is less stable in the presence of substrate. 相似文献
1000.
Moise A André S Eggers F Krzeminski M Przybylski M Gabius HJ 《Journal of the American Chemical Society》2011,133(38):14844-14847
Clinically relevant bioactivities of human galectins (adhesion/growth-regulatory galactoside-specific lectins) inspired the design of peptides as new tools to elicit favorable effects (e.g., in growth control) or block harmful binding (e.g., in tissue invasion). To obtain the bioinspired lead compounds, we combined a proteolytic fragmentation approach without/with ligand contact (excision) with mass spectrometric identification of affinity-bound protein fragments, using galectin-1 and -3 as models. Two peptides from the carbohydrate recognition domains were obtained in each case in experimental series rigorously controlled for specificity, and the [157-162] peptide of galectin-3 proved to be active in blocking lectin binding to a neoglycoprotein and to tumor cell surfaces. This approach affords peptide sequences for structural optimization and intrafamily/phylogenetic galectin comparison at the binding-site level with a minimal requirement of protein quantity, and it is even amenable to mixtures. 相似文献