TILLING技术的最新进展及其在辐射诱变育种中的应用前景

定向诱导基因组局部突变技术(Targeting Induced Local Lesions IN Genomes, 简称TILLINC)是一种全新的、高通量和低成本反向遗传学研究方法。近年来, 随着突变筛选技术的革新, TILLING技术平台日趋多元化, 使得TILLING技术的操作更为简单﹑快速, 并广泛应用于作物育种研究领域。简要介绍了TILLING技术平台的最新发展动态, 并初步探讨了将辐射诱变处理与TILLING高通量筛选相结合在诱变育种中的应用前景。To investigate the M1 biological effects of heavy ions irradiation on Lycopersicon esculentum Mill., its seeds were irradiated by 12C6+heavy ions (80 MeV/u) with the dosages of 30, 60, 90, 120 and 160 Gy respectively . The results showed that with doses increased gradually, germination rate and seedling rate of Lycopersicon esculentum Mill. were decreased, and the latter was lower than the former, mainly due to the inhibition of root growth. The irradiation increased the content of MDA and proline evidently, showing irradiation could damage biomembrane, and also decreased the activities of POD and SOD with distinct inhibition pattern. However, the low dose and high dose irradiation promoted APX activity, illustrating APX was induced to protect irradiation injury. In brief, exposure to 12C6+ heavy ions had obvious injury effects on the seeds of Lycopersicon esculentum Mill.. Heavy ions irradiation damaged biomembrane, inhibited activities of enzymes, and finally inhibited the growth of the first generation of these seeds.     

用飞秒激光在透明介质体内形成衍射光栅

用数值孔径为0.65的40倍显微透镜紧聚焦能量稍高于能量损伤阈值的200 fs的钛宝石激光脉冲,分别在ZK6玻璃和K9玻璃内部导致局部折射率改变,在透明介质内部形成透射式相位衍射光栅．在635 nm的He-Ne激光照射下,通过对光栅衍射效率的数值拟合计算,得出了的光栅条纹宽度与实际测量基本吻合,这从侧面证明了折射率改变区域为超高斯型的假定．还对折射率改变区域的性质进行了分析．在实验上观察到脉冲间的相互影响将导致折射率改变区域范围的增大．从理论上给出了增加1级衍射效率的途径．