Canady Cold Helios Plasma Reduces Soft Tissue Sarcoma Viability by Inhibiting Proliferation,Disrupting Cell Cycle,and Inducing Apoptosis: A Preliminary Report

Soft tissue sarcomas (STS) are a rare and highly heterogeneous group of solid tumors, originating from various types of connective tissue. Complete removal of STS by surgery is challenging due to the anatomical location of the tumor, which results in tumor recurrence. Additionally, current polychemotherapeutic regimens are highly toxic with no rational survival benefit. Cold atmospheric plasma (CAP) is a novel technology that has demonstrated immense cancer therapeutic potential. Canady Cold Helios Plasma (CHCP) is a device that sprays CAP along the surgical margins to eradicate residual cancer cells after tumor resection. This preliminary study was conducted in vitro prior to in vivo testing in a humanitarian compassionate use case study and an FDA-approved phase 1 clinical trial (IDE G190165). In this study, the authors evaluate the efficacy of CHCP across multiple STS cell lines. CHCP treatment reduced the viability of four different STS cell lines (i.e., fibrosarcoma, synovial sarcoma, rhabdomyosarcoma, and liposarcoma) in a dose-dependent manner by inhibiting proliferation, disrupting cell cycle, and inducing apoptosis-like cell death.     

Investigation of the Uptake and Transport of Two Novel Camptothecin Derivatives in Caco-2 Cell Monolayers

Irinotecan and Topotecan are two Camptothecin derivatives (CPTs) whose resistance is associated with the high expression of breast cancer resistance protein (BCRP) and P-glycoprotein (P-gp). To reverse this resistance, two novel CPTs, FL77-28 (7-(3-Fluoro-4-methylphenyl)-10,11-methylenedioxy-20(S)-CPT) and FL77-29 (7-(4-Fluoro-3-methylphenyl)-10,11-methylenedioxy-20(S)-CPT), were synthesized by our group. In this study, the anti-tumor activities of FL77-28, FL77-29, and their parent, FL118 (10,11-methylenedioxy-20(S)-CPT), were evaluated and the results showed that FL77-28 and FL77-29 had stronger anti-tumor activities than FL118. The transport and uptake of FL118, FL77-28, and FL77-29 were investigated in Caco-2 cells for the preliminary prediction of intestinal absorption. The apparent permeability coefficient from apical to basolateral (P_{app AP-BL}) values of FL77-28 and FL77-29 were (2.32 ± 0.04) × 10⁻⁶ cm/s and (2.48 ± 0.18) × 10⁻⁶ cm/s, respectively, suggesting that the compounds had moderate absorption. Since the transport property of FL77-28 was passive diffusion and the efflux ratio (ER) was less than 2, two chemical inhibitors were added to further confirm the involvement of efflux proteins. The results showed that FL77-28 was not a substrate of P-gp or BCRP, but FL77-29 was mediated by P-gp. In conclusion, FL77-28 might be a promising candidate to overcome drug resistance induced by multiple efflux proteins.     

Application of Two Novel Acoustic Emission Parameters on Identifying the Instability of Granite

The issue of monitoring and early warning of rock instability has received increasing critical attention in the study of rock engineering. To investigate the damage evolution process of granite under triaxial compression tests, acoustic emission (AE) tests were performed simultaneously. This study firstly introduced two novel parameters, i.e., the coefficient of variation (CoV) of the information entropy and correlation dimension of the amplitude data from the AE tests, to identify the precursor of the failure of granite. Then the relationship between the changes in these parameters and the stress-time curve was compared and analyzed. The results of this study show that: (1) There is a strong correlation between the CoV of the information entropy and the failure process of granite. The granite failed when the CoV curve raised to a plateau, which could be used as an indicator of rock instability. (2) The fluctuation of the correlation dimension indicates the different stages during the loading process, i.e., the initial compaction stage, the linear elastic stage, the yield stage, and the failure stage. Each stage contains a descending and a rising process in the correlation dimension curve, and the exhibited starting point or the bottom point at the correlation dimension curve could be selected as the indicator point for the rock instability. (3) The combined analysis of the Information entropy and Correlation dimension can improve the accuracy of rock instability prediction. This study provides new insights into the prediction of rock instability, which has theoretical implications for the stability of subsurface engineering rock masses.     

Preparation of nano-hydroxyapatite/poly(<Emphasis Type="SmallCaps">l</Emphasis>-lactide) biocomposite microspheres

Nano-hydroxyapatite (HA)/poly(l-lactide) (PLLA) composite microspheres with relatively uniform size distribution were prepared by a solid-in-oil-in-water (s/o/w) emusion solvent evaporation method. The encapsulation of the HA nanopaticles in microshperes was significantly improved by grafting PLLA on the surface of the HA nanoparticles (p-HA) during emulsion process. This procedure gave a possibility to obtain p-HA/PLLA composite microspheres with uniform morphology and the encapsulated p-HA nanoparticle loading reached up to 40 wt% (33 wt% of pure HA) in the p-HA/PLLA composite microspheres. The microstructure of composite microspheres from core-shell to single phase changed with the variation of p-HA to PLLA ratios. p-HA/PLLA composite microspheres with the diameter range of 2–3 μm were obtained. The entrapment efficiency of p-HA in microspheres could high up to 90 wt% and that of HA was only 13 wt%. Surface and bulk characterizations of the composite microspheres were performed by measurements such as wide angle X-ray diffraction (WAXD), thermal gravimetric analysis (TGA), environmental scanning electron microscope (ESEM) and transmission electron microscopy (TEM).     

A novel optical technique for realizing real-time Logarithmic transformation

We propose a novel optical technique for performing real-time nonlinear transformation and particularly for realizing Logarithmic transformation     

Wavefront reconstruction of Fresnel diffraction field by optical methods and digital algorithm

The experimental methods for measuring the phase distribution of Fresnel diffraction field by using interference and polarization information are given in this paper. An effective algorithm for phase retrieval under Fresnel-zone transform is also presented.     

ICP－AES法测定钨酸钇钾激光晶体中的Er和Nd

应用ＩＣＰ－ＡＥＳ法测定钨酸钇钾［ｋＹ（ＷＯ４）２］激光晶体中的Ｅｒ和Ｎｄ。试样用磷酸分解，Ｔｍ被选作内标元素。Ｅｒ和Ｎｄ的回收率分别为９８．６％－１０５．３％和９６．５％－１０５．８％，相对标准偏差分别为１．９０％和１．６０％。     

基于拓扑结构的碱金属化合物摩尔磁化率的支持向量回归研究

基于经典电动力学导出的表征简单离子磁化率的磁性点价g_i所构建的分子磁性连接性指数?^mF及45种碱金属化合物的摩尔磁化率χ_m的实测数据集,利用粒子群寻优的支持向量回归（SVR）方法,建立了基于⁰F和¹F的碱金属化合物χ_m的预测模型,并与基于多元线性回归（MLR）模型的计算结果进行了比较.结果显示,基于9次交叉验证的SVR模型预测的平均绝对误差、平均相对误差绝对值以及均方根误差均比MLR模型小,表明SVR模型的回归预测能力优于MLR.研究表明,磁性连接性指数^mF是一种合适的分子描述符,SVR是一种预测碱金属化合物χ_m的有效方法. 关键词： 碱金属化合物 摩尔磁化率 支持向量回归 预测     

Investigation of the limit of lateral beam shifts on a symmetrical metal-cladding waveguide

This paper reports that Goos--H\"anchen (GH) shifts occurring on a symmetrical metal-cladding waveguide are experimentally identified. It was found that there exists a critical thickness of the upper metal layer, h_cr, above which negative shift is observed and, reversely, positive shift occurs. Both positive and negative GH shifts near the critical thickness do not vary dramatically and can achieve a maximum on the submillimeter scale, which is different from simulated results using the stationary-phase method. It also shows that this critical thickness, h_cr, can be obtained at the position for zero reflectivity by setting the intrinsic damping to be the same as the radiative damping. The GH effects observed near the critical thickness are produced by extreme distortion of the reflected beam profiles, which limits the amplitude of the GH shift and, further, the sensitivity of the GH optical sensor based on the symmetrical metal-cladding waveguide.     

近场宽带电场耦合天线的高频结构模拟器软件仿真及性能分析

近场宽带电场耦合天线是一种新型的短距离、高速无线通信天线,它是索尼公司力推的近距离高速无线通信技术——.Transferjet^TM的工作天线.使用高频结构模拟器软件,对一种典型的近场宽带电场耦合天线进行了参数化的结构设计;通过仿真模拟,分析短截线的线长和线宽,以及耦合电极的面积对天线中心频率和工作带宽的影响;分析了天线间的距离和夹角,以及耦合电极的面积,对天线传输损耗和性能的影响.设计了一个满足Transferjet^TM技术指标的小尺寸天线,给出了性能仿真结果.