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11.
Chunxia Zhao Guowang Xu Xianzhe Shi Jianmei Ma Yan Zhang Shen Lv Qianfeng Weng Qing Yang 《Analytical sciences》2004,20(7):1001-1005
Fluorescent-based single-strand conformation polymorphism (F-SSCP) analysis with capillary electrophoresis (CE) is the most common method for the detection of mutation because of its high sensitivity and resolution. In this study, we prepared an inexpensive linear polyacrylamide (LPA), and successfully applied it to CE-SSCP analysis and tandem CE-SSCP/heteroduplex analysis (HA) of the P53 gene on an ABI capillary genetic analyzer. A comparison of the sieving capabilities of a homemade LPA and commercial polydimethylacrylamide (PDMA) demonstrates that the homemade LPA has a higher resolution, a shorter analysis time, and is more suitable for tandem SSCP/HA than commercial PDMA. To show the usefulness, mutations of P53 gene exon 7 - 8 in 37 tumor samples were investigated by using homemade LPA. The results indicate that 10 mutations were found in 9 of 37 cases; the majority of P53 mutations were missense mutations, and 70% were located in exon 7, which plays an important role in neoplastic progression in human tumorigenesis. 相似文献
12.
13.
Gui Hong YAN Da XING* Shi Ci TAN Institute of Laser Life Science South China Normal University Guangzhou 《中国化学快报》2004,15(1):101-104
Electrochemiluminescence (ECL), like other chemiluminescence technique, offers high signal-to-noise ratio. Furthermore, it has the advantage over other chemiluminescence techniques of being initiated by a voltage potential. Thus, it provides a better-controlled luminescence. This technique has been used in measuring many kinds of organic and inorganic matters, and analyzing many kinds of antigen, antibody and hapten, such as carcinoembryonic antigen and alpha-fetoprotein, etc1-6. The ele… 相似文献
14.
The plastic deformation field near the horizontal surface of a half space of perfectly plastic material associated with an indenting rotating rigid tooth is studied using slip line theory. The tooth is one of many on a roll surface and indents first into the half-space material and then rotates about the roll center. A slip line field is proposed for the deforming plastic region and a solution scheme is outlined. The emphasis is on determining the shape of the deforming region, especially that of the free surface. The study has a potential application in roughness transfer in metal forming processing. 相似文献
15.
We describe a scheme of stochastic implementations of quantum teleportation and entanglement swapping in terms of neutral kaons. In this scheme, the kaon whose state is to be teleported collides with one of the two entangled kaons in an Einstein–Podolsky–Rosen state. Subsequent detection of the outgoing particles of the collision completes the two-qubit projection on Alice side. There appear novel features, which connects quantum information science with fundamental laws of particle physics. 相似文献
16.
Cao H. P. Chen G. Grechkoseeva M. A. Mazurov V. D. Shi W. J. Vasil'ev A. V. 《Siberian Mathematical Journal》2004,45(6):1031-1035
The spectrum of a finite group is the set of its element orders. A finite group G is said to be recognizable by spectrum, if every finite group with the same spectrum as G is isomorphic to G. The purpose of the paper is to prove that for every natural m the finite simple Chevalley group F
4(2
m
) is recognizable by spectrum. 相似文献
17.
This paper is to investigate the approximate confidence limits of the reliability performances (such as failure rate, reliability function and average life) for a cold standby series system. The Bayesian approximate upper confidence limit of failure rate is obtained firstly, and next Bayesian approximate lower confidence limits for reliability function and average life are presented. The expressions for calculating Bayesian lower confidence limits of the reliability function and average life are also obtained, and an illustrative example is examined numerically by means of the Monte-Carlo simulation. Finally, the accuracy of confidence limits is discussed. 相似文献
18.
ChunXiaLI YingXiaLI LingBoYU HuaZHANG SiftDongCHU HuaShiGUAN 《中国化学快报》2003,14(8):776-778
A convenient synthetic route was developed for the synthesis of the novel glycolipids: 1,2-di-O-acyl-3-O-(2‘-acylamide-2‘-denxy-ct-D-glucopyranosyl)-sn-glycerols. 10 new compounds of glycolipids with different acyl groups were obtained. 相似文献
19.
5‐Methylene‐2‐oxazolidinone (1) and 5‐methylene‐1,3‐thiazolidine‐2‐thione (4) react with various isocyanates to give the corresponding urethanes 3 and 5 in high yields in the presence of palladium(0) or palladium(II) catalyst under mild reaction conditions. A mechanism is proposed. Copyright © 2003 John Wiley & Sons, Ltd. 相似文献
20.
YunFei Liu ShengCai Shi ShengLin Yu 《International Journal of Infrared and Millimeter Waves》2003,24(12):2069-2077
When electromagnetic waves propagate through a wire grid, there will be some evanescent space harmonics generated around the wire grid. In this paper, we mainly investigate their effects on the transmission characteristics of a Gaussian beam by cylindrical wire grids. The results are compared with those without taking account of evanescent space-harmonics. 相似文献