A quick method for the simultaneous determination of ascorbic acid and sorbic acid in fruit juices by capillary zone electrophoresis

A method of quickly determining ascorbic acid and sorbic acid by capillary zone electrophoresis with ultraviolet detection was developed. The choice of background electrolyte, wavelength, injection time and applied voltage were discussed. Ascorbic acid and sorbic acid were well separated in 80 mmol L⁻¹ boric acid-5 mmol L⁻¹borax (pH = 8.0) in 5 min at the detecting wavelength of 270 nm. Under the optimum condition, the method has linear ranges of 2.54-352.00 mg L⁻¹ for ascorbic acid and 1.08-336.39 mg L⁻¹ for sorbic acid with the detection limit of 1.70 mg L⁻¹ for ascorbic acid and 0.54 mg L⁻¹ for sorbic acid, respectively. Other organic acids in fruit juices have no effect on the detection. This method is very feasible and simple and can be used to detect ascorbic acid and sorbic acid in fruit juices.     

DNA TURNOVER IN BUFFER-HELD ESCHERICHIA COLI AND ITS EFFECT ON REPAIR OF UV DAMAGE

Abstract— Continuous DNA degradation and resynthesis, without a net change in cellular DNA content, were observed in buffer-held, non-irradiated E. coli B/r. This constant DNA turnover probably involves most of the genome and reflects random sites of DNA repair due to the polA-dependent excision-resynthesis repair pathway. Under these non-growth conditions, it appears that at any given time there is a minimum of one repair site per 6.5 × 10⁶ daltons DNA, each of which is at least 160 nucleotides long.
While the amount of DNA degradation is not influenced by prior exposure to UV radiation, the synthetic activity decreases with increasing UV fluence. We suggest that when sites of DNA turnover occur opposite to cyclobutyl dipyrimidines in UV-irradiated cells, repair of the latter damage can be prevented. This implies that both beneficial and deleterious processes take place in irradiated buffer-held cells, and that cell survival depends on the delicate balance between DNA turnover and repair of UV-damage. Based on these findings, we propose a model to explain the limited repair observed during post-irradiation liquid-holding and to account for the large difference in cell survival between irradiation at low fluence rates (fluence-rate dependent recovery) and at high fluence rates followed by liquid-holding (liquid-holding recovery).     

再论Pedoe不等式的高维推广及应用

本文对欧氏空间E~n中的两个n维单形,给出了著名的Pedoe不等式的一个实质性推广,并讨论了它的应用.     

波束开角对多普勒测频结果的影响*

具有不同波束开角的声学多普勒测速设备未标定的测速结果存在偏差。基于水底椭圆散射模型研究了波束开角对声学多普勒测速结果的影响,对提高声学测速性能具有实际意义。水底椭圆散射模型下,足印上不同方位传播损失和散射强度存在差异,导致频谱不对称,测频结果偏小。利用不对称系数量化频谱不对称程度,若波束开角增大,方位不对称系数随之增大,测频偏差增大,推导了波束开角导致的测频偏差解析式。分析仿真和外场试验数据测频结果可知,不同多普勒频移下测频偏差和理论计算值相符,不同波束开角下实际测频偏差与理论公式计算的测频偏差均随波束开角增大而增大。     

An Upper Bound for the w-Weak Global Dimension of Pullbacks

Let R be a commutative ring with identity and I0 an ideal of R.We introduce and study the c-weak global dimension c-w.gl.dim(R/I0) of the factor ring R/I0.Let T be a w-linked extension of R,and we also introduce the wR-weak global dimension wR-w.gl.dim(T) of T.We show that the ring T with wR-w.gl.dim(T) =0 is exactly a field and the ring T with wR-w.gl.dim(T) ≤ 1 is exactly a PwRMD.As an application,we give an upper bound for the w-weak global dimension of a Cartesian square (RDTF,M).More precisely,if T is w-linked over R,then w-w.gl.dim(R) ≤ max{wR-w.gl.dim(T) + w-fdR T,c-w.gl.dim(D) + w-fdn D}.Furthermore,for a Milnor square (RDTF,M),we obtain w-w.gl.dim(R) ≤ max{wR-w.gl.dim(T) + w-fdR T,w-w.gl.dim(D) + w-fdR D}.     

Growth regulation of luminescent gold nanoparticles directed from amphiphilic block copolymers:highly-controlled nanoassemblies toward tailored in-vivo transport

The understanding of amphiphilic block copolymers(ABC)in encapsulation and transport of inorganic nanomedicines is highly desired.Still,it remains limited due to the challenges in the fabrication of nanoassemblies(NAs)with highly-controlled shape and loading of nanoparticles.Herein,through growth regulation of luminescent gold nanoparticles(Au NPs)by different reductants with ABC pluronic F127 as a template,a straightforward strategy is reported for in-situ fabrication of three wellcontrolled gold NAs(Au NAs)that display tunable shapes from spherical to elongated nanostructures and controllable surface chemistry and loading of Au NPs with distinct emissions but identical individual Au NP size.The three Au NAs exhibit tailored invivo transport behaviours:those with spherical shape and more hydrophilic surface show longer blood retention with higher tumor-targeting efficiency(~25.3%injection dose/g)and excellent long-term near-infrared tumor imaging even after 96 h postinjection.These findings provide a useful guidance in designing specific nanostructures for future nanomedicine transport.     

In Vitro and In Vivo Antibacterial Activity,Toxicity and Resistance Analysis of Pleuromutilin Derivative Z33 against Methicillin-Resistant Staphylococcus aureus

The novel pleuromutilin derivative, which showed excellent in vitro antibacterial activity against MRSA, 22-(2-(2-(4-((4-(4-nitrophenyl)piperazin-1-yl)methyl)-1H-1,2,3-triazol-1-yl)acetamido)phenyl)thioacety-l-yl-22-deoxypleuromutilin (Z33), was synthesized and characterized in our previous work. In this study, the preliminary pharmacodynamics and safety of Z33 were further evaluated. In in vitro antibacterial activity assays, Z33 was found to be a potent bactericidal antibiotic against MRSA that induced dose-dependent growth inhibition and long-term post-antibiotic effect (PAE). The drug-resistance test demonstrated that Z33 possessed a narrow mutant selection window and lower propensities to select resistance than that of tiamulin. Cytochrome P450 (CYP450) inhibition assay determined that the inhibitory effect of Z33 was similar to that of tiamulin against the activity of CYP3A4, and was lower than that of tiamulin on the activity of CYP2E1. Toxicity determination showed that both Z33 and tiamulin displayed low cytotoxicity of RAW264.7 cells. Furthermore, Z33 was found to be a high-security compound with a 50% lethal dose (LD₅₀) above 5000 mg/kg in the acute oral toxicity test in mice. In an in vivo antibacterial activity test, Z33 displayed better therapeutic effectiveness than tiamulin in the neutropenic mouse thigh infection model. In summary, Z33 was worthy of further development as a highly effective and safe antibiotic agent against MRSA infection.     

高性能激光全息防伪标识特性参数测量系统

提出了一种高性能激光全息防伪标识参数的检测方法,利用同一束光在分束器分成的反射光和透射光光强度比例恒定的性质,实时探测衍射方向光强和标识入射光强值,通过一维线性扫描实现激光全息防伪标识衍射效率和信噪比的自动测量。实验数据表明：入射光强测量值相对误差不超过0．29％;衍射效率及信噪比的测量值最大相对误差分别不超过0．81％和2．0％,比第一代检测仪采用的方法测量精度提高近一倍。该方法降低了对光源稳定度的要求,大大降低了激光器的成本,有利于测量仪器的推广。     

Some minimax theorems with strictly monotone transformation

Some two-function minimax theorems are proved. In these results, the staircase and quantitative-topological conditions of both functions involve strictly monotone transformation and mixing of functional values. Consequently, Lin Quan and Kindler＇s minimax theorems are generalized.