An HPLC and EPR investigation on the stability of DMPO and DMPO spin adducts in vivo

Application of the spin trapping technique in intact animals requires an understanding of the stability and distribution of the spin traps and their spin adducts in vivo. We studied the stability of DMPO in vivo in mice using HPLC and the stability of spin adducts of DMPO by EPR in plasma, whole blood, peritoneal fluid, and homogenized heart tissue of the rat. At 15 minutes after intraperitoneal injection DMPO had similar concentrations in the liver, heart, and blood of the mice and 40% remained in the organs 2 hours after the injection. In contrast, the spin adduct DMPO-OH was short lived, with a half-life of 3.0 minutes in plasma, and was not detectable 1 minute after formation in whole blood and homogenized heart tissue. The carbon centered spin adduct DMPO-CH(OH)CH₃ was more stable, having half-lives of 16, 11, 3.6, and 0.79 minutes in plasma, peritoneal fluid, whole blood, and homogenized heart tissue, respectively. The spin adduct DMPO-SO₃ was sufficiently stable for the adduct to be observed directly from living mice.     

The general nucleolus and the reduced game property

The nucleolus of a TU game is a solution concept whose main attraction is that it always resides in any nonempty -core. In this paper we generalize the nucleolus to an arbitrary pair (,F), where is a topological space andF is a finite set of real continuous functions whose domain is . For such pairs we also introduce the least core concept. We then characterize the nucleolus forclasses of such pairs by means of a set of axioms, one of which requires that it resides in the least core. It turns out that different classes require different axiomatic characterizations.One of the classes consists of TU-games in which several coalitions may be nonpermissible and, moreover, the space of imputations is required to be a certain generalized core. We call these gamestruncated games. For the class of truncated games, one of the axioms is a new kind ofreduced game property, in which consistency is achieved even if some coalitions leave the game, being promised the nucleolus payoffs. Finally, we extend Kohlberg's characterization of the nucleolus to the class of truncated games.     

On locally optimal nonparametric tests

Summary There is an abundancy of problems in which no parametric model realistically describes the situation and in which, accordingly, we have to resort to nonparametric methods. As the numerical problems connected with nonparametric tests are becoming less and less important, rank tests, permutation tests and the like are becoming more and more part of the standard armatory of applied statisticians. The lack of tabulated critical values, for instance, should no longer be a serious objection against the use of permutation tests in practice; cf. Edgington (1987).The rationale underlying permutation and rank tests has been outlined in quite a number of text books and papers; cf. Fraser (1957), Lehmann (1959), Hájek-Sidák (1967) or Witting (1970). Roughly speaking, permutation tests are constructibel if the data can be condensed by means of a sufficient and complete statistic allowing for the proper kind of conditioning. Rank tests arise if the underlying problem is invariant with respect to (w.r.t.) a large group of transformations which leads to a maximal invariant statistic consisting of (signed) ranks.Most practical nonparametric problems, however, are too complex to be tractable by just one of those approaches. Many of them, however, can be handled by a combination of both techniques. In this paper we outline the logic underlying that combined reduction method and apply it to construct locally most powerful tests. Moreover, we discuss what we label Hoeffding's transfer problem, i.e. the uniformity aspect of locally most powerful tests with respect to the starting point at the boundary. We are concentrating on the discussion of the nonparametric two-sample location and scale problem. Further important problems are mentioned in Section III.This is a written account of an invited lecture delivered by the third author on occasion of the 14th Symposium über Operations Research, Ulm, September 6–8, 1989.     

L.P.-games with sufficiently many players

L.P.-games aren-person cooperative games arising from a linear program as studied by Owen and Billera/Raanan. Employing a version of nondegeneracy of games developed by Weidner and the author, we show that methods from the Geometry of Numbers are the suitable tool in order to obtain lower bounds for the number of players of each type that ensure that the core and the competitive equilibrium coincide.     

Luminescent heteronuclear Au(I)5Ag(I)8 complexes of [1,2,3-C6(C6H4R-4)3]3- (R = H, CH3, But) by cyclotrimerization of arylacetylides

Unusual AuI-AgI heterometallic complexes [Au5Ag8(mu-dppm)4{1,2,3-C6(C6H4R-4)3}(CCC6H4R-4)7]3+ (R = H 1, CH3 2, But 3) were isolated by reactions of polymeric silver arylacetylides (AgCCC6H4R-4)n with binuclear gold component [Au2(mu-dppm)2(MeCN)2]2+ (dppm = bis(diphenylphosphino)methane), in which cyclotrimerization of arylacetylide -CCC6H4R-4 affords trianion {1,2,3-C6(C6H4R-4)3}3- with an unprecedented mu5-bonding mode. Compounds 1(SbF6)3-3(SbF6)3 exhibit intense photoluminescence derived from an MLCT (Au5Ag8 --> CCC6H4R-4) transition, mixed with a metal cluster-centered excited states.     

Ring-opening reactions of arylvinylidenecyclopropanes with diaryl diselenide upon heating: formation of 1,2-diarylselenocyclopentene derivatives

Arylvinylidenecyclopropanes undergo a novel reaction upon heating at 150 °C with diaryl diselenide to give the corresponding 1,2-diarylselenocyclopentene derivatives in good to high yields within 1.5 h. The further transformation of 1,2-diarylselenocyclopentene derivatives has been disclosed.     

V-positivity,poverses and the economic global unicity theorems of Gale and Nikaido

Summary Strong theorems concerning globally unique solutions to nonlinear inequalities have been obtained byGale andNikaido via P-matrix characterizations of theJacobians of the mappings involved. We introduce two new concepts here: v-positivity and the poverse. These permit us to state and prove significant generalizations of the theorems just mentioned and, equally important, provide access to preliminary results concerning linear inequalities by the powerful direct techniques of linear programming theory.

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Zusammenfassung Gale undNikaido haben starke Sätze über globale, eindeutige Lösungen nichtlinearer Ungleichungen erhalten für den Fall, daß dieJacobi- Matrix der auftretenden Abbildung eineP-Matrix ist. Darauf aufbauend werden zwei neue Konzepte vorgestellt: v-positivity und die poverse. Diese erlauben es, Verallgemeinerungen der erwähnten Sätze aufzustellen und zu beweisen und, was von gleicher Bedeutung ist, die Verbindung zu früheren Ergebnissen über lineare Ungleichungen durch die wirkungsvollen direkten Verfahren der Theorie der Linearen Optimierung herzustellen.

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This Research was partly supported by Project No. NR 047-021, ONR Contracts NO 0014-67-A-0126-0008 and NO 0014-67-A-0126-0009 with the Center for Cybernetic Studies, The University of Texas.     

激光诱导击穿光谱(LIBS)法微损快速检测党参中Pb

中药材重金属元素快速检测对污染监控及人们健康具有重要意义。激光诱导击穿光谱技术(Laser Induced Breakdown Spectroscopy, LIBS)属于一种快速检测方法,研磨压片等预处理方法相对样品消解已有所简化,但破坏了样品的物理性质,且不能满足中药材大宗品种、大批量检测需求。若进一步简化样品预处理,将更加凸显LIBS快速检测的优势。本文建立了激光诱导击穿光谱技术(LIBS)快速微损检测中药材样品重金属元素定标方法。线性相关系数R2为0.7764,建立的微损定标曲线线性可用于切片党参LIBS快速检测,对待测党参切片样品检测平均相对误差为3.74%,与电感耦合等离子体质谱法(ICP-MS)对比,相关系数R2为0.7957,验证了LIBS技术微损检测的可行性。制备的党参参考定标样品可多次重复用于待测样定标和仪器标定等。实验对待测党参样品仅进行切片处理,避免了研磨、压片等预处理,更加充分地体现LIBS快速检测的优势,为LIBS技术应用于中药材重金属元素快检等领域提供了一种新方法。