New steps in acute coronary syndrome and antihyperlipidemic treatment: Determination of statins and metabolites by liquid chromatography tandem mass spectrometry

Our aim in this study was to develop a validated liquid chromatography–tandem mass spectrometry (LC–MS/MS) method for the measurement of atorvastatin, rosuvastatin and their major metabolites, and furthermore to evaluate patients’ adherence to statin therapy and to investigate the effect of statin therapy on various hematological and biochemical parameters. A simple protein precipitation was performed for the extraction of analytes and the extracted samples were injected directly. The levels of drugs and their metabolites were measured by the validated method in a total of 210 patients diagnosed with unstable angina pectoris (USAP), ST-elevation myocardial infarction (STEMI) or non-ST-elevation myocardial infarction (NSTEMI). Various biochemical and hematological parameters were measured. The linearity ranges for atorvastatin and rosuvastatin were 1.22–2,500 and 0.97–2000 ng/ml, respectively. The inter-assay coefficient of variation for all analytes was ≤ 6%. In patients diagnosed with USAP, STEMI and NSTEMI, treatment compliance rates were 22.1, 23.5 and 41.2% for atorvastatin and 36.1, 40.2 and 67.1% for rosuvastatin, respectively. An economical, simple and reliable measurement method has been developed. Our findings support the poor patient compliance with statin therapy in the included population. It was observed that 6 months of statin treatment caused slight changes in biochemical and hematological parameters.     

Proton conductivity properties of acid doped fluoroalkylated 1,2,3-triazole

Novel proton conducting organic electrolyte containing fluoroalkylated 1,2,3-triazole was synthesized via intramolecular cyclisation of vinyl azides. FT-IR, elemental analysis and NMR methods were used for the characterization of the resulting organic molecule. Triazole containing sample was doped with triflic acid to obtain proton conducting organic electrolytes. Thermal stability of these materials was analyzed with thermogravimetric analysis (TGA) and the melting temperatures were measured by differential scanning calorimetry (DSC). The effect of acid content on the proton conductivity was investigated with impedance spectrometer and the maximum proton conductivity was measured as 10⁻² S/cm at 150 °C.