Real-time Quantitative RT-PCR for CT9 Level in Human Cancer

Introduction Malignantdiseasesarecharacterizedbytheunreg ulatedgrowthoftransformedcells.Inrecentyears,dramaticinsightsintothemolecularmechanismsofthis phenomenonhavebeenachievedfrombasiccancerre search.Manycellularfunctionsareregulatedbychan gesingeneexpr…     

甲烷氧化偶联催化剂La2O3／SrCO3—BaCO3的催化活性与表征

在甲烷氧化偶联制Ｃ２烃的研究中，人们发现碱土金属与稀土金属组成的复合氧化物催化剂具有高的催化活性［１，２］．稀土化合物作为有希望的工业催化剂之一，已受到广泛的关注．一般认为，它们含有的氧空位对活化甲烷的有效氧物种有利．Ｄｅｂｅｙ等在１％Ｓｒ／Ｌａ２Ｏ...     

Pressurized gradient capillary electrochromatographic separation of eighteen amino acid derivatives

A pressurized gradient capillary electrochromatography (pCEC) instrument was developed to separate 18 amino acid derivatives. A reversed-phase C18 column (3 microm, 130 mm x 75 microm I.D.) and an acetate buffer (50 mmol/l NaAc, pH 6.4) with an ion-pair reagent (1% N,N-dimethylformamide) were used to separate derivatized amino acids from a standard solution (2 microg/ml), and the wavelength of the UV-Vis detector was 360 nm. The pressure on the capillary column was kept at approx. 70 Pa and 3 kV positive voltage was added on the outlet end of column. The effect of voltage on the eluting order of amino acids and the resolution of separation were studied, and it was found that when the voltage was higher than 3 kV, the adsorption of amino acids in the porous C18 column occurred. The effect of salt concentration, injection volume, and column length on the separation of amino acids was determined. The amino acid sample was separated by pCEC, and RSDs of the migration times of each amino acid were all less than 2.5%.     

SOLID PHASE SYNTHESIS OF ISOXAZOLINES

The solid-phase synthesis of isoxazolines on 2-polystyrylsulfonamidoethanol resin isreported. 2-Polystyrylsuifonamidoethanol resin 1 was reacted with acryloyl chloride to afford2-polystyrylsulfonylamidoethyl acrylate resin 2, which was further reacted with brominatedaldoximes by [3+2] cycioaddition to give isoxazoline resin 4. Resin 4 was treated with aqueous 6mol/L HCI solution to obtain isoxazolines in good yield and purity.     

Preparation of multihollow P(St-MAA) particles by sequence soap-free/soap emulsion polymerization and followed by stepwise alkali/acid posttreatment

The effects of ionic emulsifier, sodium dodecylbenzene sulfate (SDBS), on the formation of the multihollow structures in sub-micron sized polymer particles produced by alkali/acid posttreatment were investigated. The original latex particles with narrow size distribution were synthesized by a new sequence emulsifier-free/emulsifier emulsion copolymerization of styrene (St) and methacrylic acid (MAA). Results indicated that the pore size decreased and the pore number increased with the increase of SDBS amount, and the morphology of the posttreated latex particles was also significantly influenced by the introducing time of SDBS in the preparation of the original latex particles, and a suitable introducing time was 3 h of polymerization.     

ON PGLAR PRODUCT OPERATOR

Let H be a Hilbert space, and let A be a linear bounded operator on H. For \(\lambda \in \rho (A)\), the \({U_\lambda } = {(A - \lambda )^{ * - 1}}(A - \lambda )\) is called polar.Produot operator. In this paper, we discuss the properties of \({U_\lambda }\) and the relation between \({U_\lambda }\) and A. We obtain tbe following results. Definition. Let B be a linear bounded operator on H, suppose \(0 \in \rho (B)\). For every \(x,y \in H\), we definite \([x,y] = (Bx,y)(H,B)\)(or (H, [·,·]) is called a non- degenerate bilinear space (it is obvious that if B=B*,then (H,B)is a space with an indefinite metric; and that if B>0, then (H,B) is a Hilbert Space. If an operator U(A) satisfies \[[Ux,Uy] = [x,y]([Ax,y] = [x,Ay]),x,y \in H\] then the operator U(A) is called a wvitary (self adjoint) on (H,B). Theorem I . Suppose A is a linear bounded operator on H, (1) If \(0 \in \rho (A)\), then \(U = {A^{ * - 1}}A\) is a unitary operator on (H,A) or (H, A*), and \(\sigma (U) = \frac{1}{{\sigma (U)}}\). (2) If there is a complex number \(\alpha \), such that \({\mathop{\rm Im}\nolimits} A \ge \alpha > 0\) then a)\(0 \in \rho (A)\), and the operator \(U = {A^{ * - 1}}A\) is a unitary on Hilbert space \((H,{\mathop{\rm Im}\nolimits} A) and 1 \in \rho (U)\);b) there exist two Hilbert spaces \((H,{v_1}),(H,{v_2})\), such that A, A* are all the unitary operator from (H,v1) onto (H,v2), and there are two spectral measures \(\{ E_\lambda ^i,\lambda \in [\alpha ,\chi ] \subset (0,2\pi )\} ,i = 1,2,\), such that \(AE_\Delta ^1H \subset E_\Delta ^2H,{A^ * }E_\Delta ^1H \subset E_\Delta ^2H\) for any \(\Delta = (\lambda ,u] \subset (0,2\pi ]\). (3) If \(0 \in \rho (A) \cap \rho ({\mathop{\rm Im}\nolimits} A)\) then the operator \(U = {A^{ * - 1}}A\) is a unitary on \((H,{\mathop{\rm Im}\nolimits} A)\). with an indefinite met He, and \(1 \in \rho (U)\). (4) For any complex number \(\lambda = r{e^{i\theta }},\left| \lambda \right| > \left\| A \right\|\), then \({U_\lambda }\) must be a unitary operator on the Hilbert space \(\left( {H,{\mathop{\rm Im}\nolimits} (\frac{1}{{i\lambda }}A + iI)} \right),and - {e^{i2\theta }} \in \rho ({U_\lambda })\) Theorem 2. (1) A is a normal operator iff there exists a complex number \(\lambda \), \(\lambda \in \rho (A)\), such that \(\frac{{\partial {U_\lambda }}}{{\partial \lambda }}{U_\lambda } = {U_\lambda }\frac{{\partial {U_\lambda }}}{{\partial \lambda }}\),where \(\frac{\partial }{{\partial \lambda }}\) is the directional derivative. (2) If there exists a complex number \(\alpha ,{\mathop{\rm Im}\nolimits} A \ge \alpha > 0\) then A is a normal operator iff \(U = {A^{ * - 1}}A\) is also. (3) If A is a hyperiwrmal or a subnormal, then for every \(\lambda \in \rho (A),\sigma ({U_\lambda })\) lies on the circle. 3, 4期 关于极?积算子炉一U 499 Theorem 3? Let A be a linear bounded operator on Hm Suppose 0￡p(J.)? (1) If U=А*~гА is a unitary operator in a certain non-degenerate bilinear space (H} 1 J |Л|>1 入 J Ш=1 solmble iff (l)cr(ü) =-=i=-, (2) the operotor Ui= f XdE\ is unitarity equivalent to cr(C7) J |л|>1 the operator ül_1 == ШЕ1, 、 J 1Л|>1 If the conditions (1),(2) are satified, then we have (3) the subspace JJi?^2 of H reduces any solution of the equation A*"1 A — U, and 1 AI (Ягея*)1 = ^Uo where Ъ is any in vertible self-adjoint on (i?i?J?2) L, and bv Uo (Uo = Jiai i ЫЕ1 )}⑷41Я1фя, = , Ла = A1ü2, if the operator V is to realize TJt and Ut^unitary equivalence^ then Ai = VSf where S is any invertible on Нг and SvUi. " Corollary. Swpp)se operator U is a normal on a certain Hilbert space(Hy v) (^the U is similar to a certain normal operator on IT), if U satisfies the conditions (1)、(2) of Theorem^ on ?S,v) ? Then the general form of the solution of А*~гА = U is A = vA'y where A' is same as A in Theorem^. Theorem 5. Let U be a linear bounded operator on H. Suppose O?p(J.)and p(ü) is a simply connected region, then the equation А*~гЛ = U is a solvable iff there exists a certain space {H,v) with a indefinite metric, such that U is a unitary operator on (Hf 丨）. If is a unitary operator on ?H,v),then there exists a particular solution of I X+$ А*~гА = U: Ar = 2e v [ (?7 —X)_1+X], where eie ? p (JJ), and the general form of the solution is Аж АУ, wliere V is any in vertible self-adjoint on (H, -u)and VvU,     

聚L-苏氨酸修饰电极对多巴胺和肾上腺素的电催化氧化

利用循环伏安法将L-苏氨酸聚合修饰在玻碳电极表面, 制成聚L-苏氨酸修饰电极. 实验表明, 该电极对多巴胺和肾上腺素都有较好的催化氧化效果. 运用循环伏安法详细研究了修饰电极的电化学性质. 在pH 2.5的磷酸盐缓冲溶液(PBS)中, 肾上腺素的电子传递系数为0.51, 表观反应速率常数为1.33 s-1; 在pH 7.5的PBS中, 多巴胺在电极上产生一对氧化还原峰, 多巴胺在电极上的电子传递系数为0.60, 表观反应速率常数为0.92 s-1. 该修饰电极对多巴胺和肾上腺素能够进行同时测定, 还原峰电流与多巴胺和肾上腺素浓度分别在1.0×10-6-5.0×10-4 mol·L-1和3.0×10-6-1.0×10-4 mol·L-1范围内呈现良好的线性关系.     

SiHCl3-H2气相外延生长Si单晶反应机理的理论研究

采用密度泛函理论计算方法, 在B3LYP/6-311G*水平下, 计算并得到了SiHCl3与H2反应各反应通道上各驻点的构型、振动频率和能量. 结果表明, 在气相中SiHCl3分解的通道d和SiHCl3与H2反应的通道c为竞争反应, 但其均未还原出Si原子, 只有衬底Si参与SiHCl3-H2的反应, Si原子才淀积在Si衬底上.     

α-乙酰氧基-N-亚硝基吡咯烷的解离及其致癌代谢机理的理论研究

用ab initio方法, 在MP2/6-31G**水平下讨论了α-乙酰氧基-亚硝基吡咯烷(α-Acetoxy-NPYR)在各种条件下的解离反应机理, 并对形成终致癌物B, C, D的代谢机理进行研究. 发现在OH^－和H₂O作用下的解离都遵循羟基进攻羰基机理, OH^－作用下是一个经四面体中间体阴离子的无位垒过程, H₂O作用下有相对高的活化能(165.36 kJ/mol). H₃O^＋作用下是先形成阳离子产物的S_N1过程, 并没有发现遵循两种综合的解离情形. 同时, 羟基化产物异构化为终致癌物B, C, D是一个相对容易进行的过程.     

Itosides A – I,New Phenolic Glycosides from Itoa orientalis

Eight new benzoylated gentisyl alcohol (=2‐(hydroxymethyl)benzene‐1,4‐diol) glucosides, itosides A–H ( 1 – 8 ), together with the new pyrocatechol (=benzene‐1,2‐diol) glycoside itoside I ( 9 ) were isolated from the bark and twigs of Itoa orientalis (Flacourtiaceae). In itosides B–D ( 2 – 4 ), the gentisyl alcohol moiety was esterified by 1‐hydroxy‐6‐oxocyclohex‐2‐ene‐1‐carboxylic acid, while itosides E–H ( 5 – 8 ) contained instead an additional 2‐hydroxybenzoic acid moiety. The compounds were accompanied by the known derivatives 4‐hydroxytremulacin ( 10 ), poliothyrsoside ( 11 ), poliothyrsin ( 12 ), homaloside D ( 13 ), tremulacin, and pyrocatechol β‐D ‐glucopyranoside. The structures of the new compounds were elucidated by spectral and chemical methods.