全文获取类型
收费全文 | 323篇 |
免费 | 11篇 |
国内免费 | 3篇 |
专业分类
化学 | 228篇 |
晶体学 | 2篇 |
力学 | 11篇 |
数学 | 46篇 |
物理学 | 50篇 |
出版年
2024年 | 1篇 |
2023年 | 4篇 |
2022年 | 6篇 |
2021年 | 14篇 |
2020年 | 2篇 |
2019年 | 8篇 |
2018年 | 2篇 |
2017年 | 4篇 |
2016年 | 9篇 |
2015年 | 11篇 |
2014年 | 13篇 |
2013年 | 25篇 |
2012年 | 18篇 |
2011年 | 22篇 |
2010年 | 9篇 |
2009年 | 20篇 |
2008年 | 22篇 |
2007年 | 24篇 |
2006年 | 20篇 |
2005年 | 17篇 |
2004年 | 14篇 |
2003年 | 6篇 |
2002年 | 8篇 |
2001年 | 7篇 |
2000年 | 6篇 |
1999年 | 3篇 |
1998年 | 4篇 |
1997年 | 4篇 |
1996年 | 3篇 |
1995年 | 1篇 |
1994年 | 3篇 |
1993年 | 1篇 |
1991年 | 3篇 |
1990年 | 4篇 |
1988年 | 3篇 |
1987年 | 1篇 |
1985年 | 3篇 |
1983年 | 1篇 |
1982年 | 1篇 |
1981年 | 3篇 |
1980年 | 1篇 |
1979年 | 1篇 |
1978年 | 1篇 |
1975年 | 1篇 |
1962年 | 2篇 |
1956年 | 1篇 |
排序方式: 共有337条查询结果,搜索用时 15 毫秒
191.
An RP-HPLC method for the analysis of adenosine (ADO) has been developed and validated. In the present study, we report an RP-HPLC-based method with modifications of mobile phase and shorter retention time that substantially improved the efficiency of ADO analysis. The HPLC separation of the ADO was achieved on a C18 column, using a mobile phase consisting of water, containing 7% v/v ACN, at a flow rate of 0.8 mL/min. The column effluent was monitored by UV detection at 260 nm. A linear response was achieved over the concentration range of 0.25-100.00 micromol/L. The analytical method inter- and intra-run accuracy and precision were better than +/- 15%. The LOQ was 0.25 micromol/L, with ADO detection in the range of 6.25 pmol per sample. The method has been applied to the study of adenosine kinase (AK) kinetics. 相似文献
192.
Recent applications in nanoliquid chromatography 总被引:1,自引:0,他引:1
Since its first introduction by Karlsson and Novotny in 1988 nano-LC has emerged as a complementary and/or competitive separation method to conventional HPLC, offering several advantages such as higher efficiency, ability to work with minute sample sizes and lower consumption of mobile phases, and better compatibility with MS, etc. Although its use was not so extended initially, in the last years new and interesting applications have appeared which deserve to be carefully considered. The aim of this review is therefore to provide an updated and critical survey of different nano-LC applications in analytical chemistry. 相似文献
193.
Rocco A Aturki Z D'Orazio G Fanali S Solínová V Hlavácek J Kasicka V 《Electrophoresis》2007,28(11):1689-1695
The separation of several insect oostatic peptides (IOPs) was achieved by using CEC with a strong-cation-exchange (SCX) stationary phase in the fused-silica capillary column of 75 microm id. The effect of organic modifier, ionic strength, buffer pH, applied voltage, and temperature on peptides' resolution was evaluated. Baseline separation of the studied IOPs was achieved using a mobile phase containing 100 mM pH 2.3 sodium phosphate buffer/water/ACN (10:20:70 v/v/v). In order to reduce the analysis time, experiments were performed in the short side mode where the stationary phase was packed for 7 cm only. The selection of the experimental parameters strongly influenced the retention time, resolution, and retention factor. An acidic pH was selected in order to positively charge the analyzed peptides, the pI's of which are about 3 in water buffer solutions. A good selectivity and resolution was achieved at pH <2.8; at higher pH the three parameters decreased due to reduced or even zero charge of peptides. The increase in the ionic strength of the buffer present in the mobile phase caused a decrease in retention factor for all the studied compounds due to the decreased interaction between analytes and stationary phase. Raising the ACN concentration in the mobile phase in the range 40-80% v/v caused an increase in both retention factor, retention time, and resolution due to the hydrophilic interactions of IOPs with free silanols and sulfonic groups of the stationary phase. 相似文献
194.
Alessandro Perrone Susan Mohamed Vincenzo Donadio Rocco Liguori Manuela Contin 《Molecules (Basel, Switzerland)》2021,26(23)
Fabry disease (FD) is a rare X-linked lysosomal storage disorder caused by α-galactosidase A gene (GLA) mutations, resulting in loss of activity of the lysosomal hydrolase, α-galactosidase A (α-Gal A). As a result, the main glycosphingolipid substrates, globotriaosylceramide (Gb3) and globotriaosylsphingosine (lyso-Gb3), accumulate in plasma, urine, and tissues. Here, we propose a simple, fast, and sensitive method for plasma quantification of lyso-Gb3, the most promising secondary screening target for FD. Assisted protein precipitation with methanol using Phree cartridges was performed as sample pre-treatment and plasma concentrations were measured using UHPLC-MS/MS operating in MRM positive electrospray ionization. Method validation provided excellent results for the whole calibration range (0.25–100 ng/mL). Intra-assay and inter-assay accuracy and precision (CV%) were calculated as <10%. The method was successfully applied to 55 plasma samples obtained from 34 patients with FD, 5 individuals carrying non-relevant polymorphisms of the GLA gene, and 16 healthy controls. Plasma lyso-Gb3 concentrations were larger in both male and female FD groups compared to healthy subjects (p < 0.001). Normal levels of plasma lyso-Gb3 were observed for patients carrying non-relevant mutations of the GLA gene compared to the control group (p = 0.141). Dropping the lower limit of quantification (LLOQ) to 0.25 ng/mL allowed us to set the optimal plasma lyso-Gb3 cut-off value between FD patients and healthy controls at 0.6 ng/mL, with a sensitivity of 97.1%, specificity of 100%, and accuracy of 0.998 expressed by the area under the ROC curve (C.I. 0.992 to 1.000, p-value < 0.001). Based on the results obtained, this method can be a reliable tool for early phenotypic assignment, assessing diagnoses in patients with borderline GalA activity, and confirming non-relevant mutations of the GLA gene. 相似文献
195.
Unai Arregui Leon Luca Carletti Davide Rocco Costantino De Angelis Giuseppe Della Valle 《Laser \u0026amp; Photonics Reviews》2024,18(2):2300669
Optical rectification (OR) at the nanoscale has attracted an increasing interest in the prospect of providing efficient ultracompact terahertz (THz) sources. Here, a universal modeling approach capable of addressing both isotropic and anisotropic all-dielectric nonlinear nanomaterials on an ultra-broad spectral range, covering the highly dispersive phonon-polariton window, and different orientations of the crystallographic axes with respect to the geometry of the structure is reported. This analysis is exemplified by considering two study cases, that is, nanopillars of AlGaAs and of LiNbO3. A close comparison between the two cases is established in terms of THz generation efficiency from 4 to 14 THz. Phonon-polariton contributions to the OR process are disentangled from the electronic one, and a model order reduction based on the reciprocity theorem is applied and validated on both the considered configurations. These results, combined with the inspection of the THz near-field features, pave the way to the design and optimization of nonlinear metasurfaces for THz generation and detection at the nanoscale. 相似文献
196.
Examples of surfaces inP
6 with no trisecant lines are constructed. A partial classification recovering them is given and conjectured to be the complete
one.
Dedicated to the memory of F. Serrano 相似文献
197.
Manuele Gori Sara Maria Giannitelli Gianluca Vadal Rocco Papalia Loredana Zollo Massimo Sanchez Marcella Trombetta Alberto Rainer Giovanni Di Pino Vincenzo Denaro 《Molecules (Basel, Switzerland)》2022,27(10)
Invasive intraneural electrodes can control advanced neural-interfaced prostheses in human amputees. Nevertheless, in chronic implants, the progressive formation of a fibrotic capsule can gradually isolate the electrode surface from the surrounding tissue leading to loss of functionality. This is due to a nonspecific inflammatory response called foreign-body reaction (FBR). The commonly used poly(ethylene glycol) (PEG)-based low-fouling coatings of implantable devices can be easily encapsulated and are susceptible to oxidative damage in long-term in vivo applications. Recently, sulfobetaine-based zwitterionic hydrogels have emerged as an important class of robust ultra-low fouling biomaterials, holding great potential to mitigate FBR. The aim of this proof-of-principle in vitro work was to assess whether the organic zwitterionic—poly(sulfobetaine methacrylate) [poly(SBMA)]—hydrogel could be a suitable coating for Polyimide (PI)-based intraneural electrodes to reduce FBR. We first synthesized and analyzed the hydrogel through a mechanical characterization (i.e., Young’s modulus). Then, we demonstrated reduced adhesion and activation of fibrogenic and pro-inflammatory cells (i.e., human myofibroblasts and macrophages) on the hydrogel compared with PEG-coated and polystyrene surfaces using cell viability assays, confocal fluorescence microscopy and high-content analysis of oxidative stress production. Interestingly, we successfully coated PI surfaces with a thin film of the hydrogel through covalent bond and demonstrated its high hydrophilicity via water contact angle measurement. Importantly, we showed the long-term release of an anti-fibrotic drug (i.e., Everolimus) from the hydrogel. Because of the low stiffness, biocompatibility, high hydration and ultra-low fouling characteristics, our zwitterionic hydrogel could be envisioned as long-term diffusion-based delivery system for slow and controlled anti-inflammatory and anti-fibrotic drug release in vivo. 相似文献
198.
Righi Michele Moretti Giacomo Forehand David Agostini Lorenzo Vertechy Rocco Fontana Marco 《Nonlinear dynamics》2021,105(4):2861-2876
Nonlinear Dynamics - Dielectric elastomer generators (DEGs) are a promising option for the implementation of affordable and reliable sea wave energy converters (WECs), as they show considerable... 相似文献
199.