Cystatins, serpins and other families of protease inhibitors in plants

Plant protease inhibitors (PIs) are generally small proteins present in high concentrations in storage tissues (tubers and seeds), and to a lower level in leaves. Even if most of them are active against serine and cysteine proteases, PIs active against aspartic proteases and carboxypeptidases have also been identified. Inhibitors of serine proteases are further classifiable in several families on the basis of their structural features. They comprise the families known as Bowman-Birk, Kunitz, Potato I and Potato II, which are the subject of review articles included in this special issue. In the present article we aim to give an overview of other families of plant PIs, active either against serine proteases or other class of proteases, describing their distribution, activity and main structural characteristics.     

Geometric continuum mechanics

Geometric Continuum Mechanics ( GCM) is a new formulation of Continuum Mechanics ( CM) based on the requirement of Geometric Naturality ( GN). According to GN, in introducing basic notions, governing principles and constitutive relations, the sole geometric entities of space-time to be involved are the metric field and the motion along the trajectory. The additional requirement that the theory should be applicable to bodies of any dimensionality, leads to the formulation of the Geometric Paradigm ( GP) stating that push-pull transformations are the natural comparison tools for material fields. This basic rule implies that rates of material tensors are Lie-derivatives and not derivatives by parallel transport. The impact of the GP on the present state of affairs in CM is decisive in resolving questions still debated in literature and in clarifying theoretical and computational issues. As a consequence, the notion of Material Frame Indifference ( MFI) is corrected to the new Constitutive Frame Invariance ( CFI) and reasons are adduced for the rejection of chain decompositions of finite elasto-plastic strains. Geometrically consistent notions of Rate Elasticity ( RE) and Rate Elasto-Visco-Plasticity ( REVP) are formulated and consistent relevant computational methods are designed.     

Shear stresses in elastic beams: an intrinsic approach

The theory of non-uniform flexure and torsion of Saint-Venant's beam with arbitrary multiply connected cross section is revisited in a coordinate-free form to provide a computationally convenient context. Numerical implementations, by Matlab, are performed to evaluate the maximum elastic shear stresses in beams with rectangular cross sections for different Poisson's ratios. The deviations between the maximum and mean stresses are then diagrammed to adjust the results provided by Jourawski's method.     

High-Energy Proton-Beam-Induced Polymerization/Oxygenation of Hydroxynaphthalenes on Meteorites and Nitrogen Transfer from Urea: Modeling Insoluble Organic Matter?

Formation and structural modification of oxygenated polycyclic aromatic hydrocarbons (oxyPAHs) by UV irradiation on minerals have recently been proposed as a possible channel of PAH transformation in astrochemical and prebiotic scenarios of possible relevance for the origin of life. Herein, it is demonstrated that high-energy proton-beam irradiation in the presence of various meteorites, including stony iron, achondrite, and chondrite types, promotes the conversion of two representative oxyPAH compounds, 1-naphthol and 1,8-dihydroxynaphthalene, to complex mixtures of oxygenated and oligomeric derivatives. The main identified products include polyhydroxy derivatives, isomeric dimers encompassing benzofuran and benzopyran scaffolds, and, notably, a range of quinones and perylene derivatives. Addition of urea, a prebiotically relevant chemical precursor, expanded the range of identified species to include, among others, quinone diimines. Proton-beam irradiation of oxyPAH modulated by nitrogen-containing compounds such as urea is proposed as a possible contributory mechanism for the formation and processing of insoluble organic matter in meteorites and in prebiotic processes.