Syntheses and Crystal Structures of Two 3D Lead(II) Coordination Polymers with Isonicotinic Acid N‐Oxide and Pseudohalides

Two novel 3D lead(II)‐azido and ‐thiocyanato coordination polymers with isonicotinate acid N‐oxide (INO) as the coligand were synthesized and characterized by elemental analysis, IR, and ¹H NMR spectroscopy and were also studied using X‐ray crystallography technique. These complexes have the formulas [Pb(INO)(NCS)]_n ( 1 ) and [Pb(INO)(N₃)(H₂O)]_n ( 2 ).     

Inhibition of Survivin Restores the Sensitivity of Breast Cancer Cells to Docetaxel and Vinblastine

Combination therapy is considered a viable strategy to overcome the resistance to chemotherapeutics. Survivin as a member of the inhibitor of apoptosis protein (IAP) family, which is involved in resistance to various drugs. We investigated the role of combination therapy in downregulating survivin and increasing drug’s efficacy in MDA-MB-231 cells. MTT assay and DAPI staining were applied to study the anti-proliferative activity and apoptosis response of the agents. Real-time RT-PCR and Western blot analysis were applied to study survivin mRNA and protein. Our findings showed that combined treatment of cells with docetaxel and vinblastine reduces survivin expression and consequently decreases the IC₅₀ value of docetaxel from 70 to 5 nM (p?p?相似文献   

N-磺酸基聚(4-乙烯吡啶)硫酸氢铵作为新型、高效、可重复使用的固体酸催化剂用于无溶剂条件下酰化反应(英文)

N-Sulfonic acid poly(4-vinylpyridinum) hydrogen sulfate has been developed as a recyclable solid acid catalyst for the acetylation of alcohols, phenols, thiols, and amines, as well as the 1,1- diacetylation of aldehydes under solvent-free conditions at room temperature. The acetylated products were formed in good to excellent yields over short reaction times, and the catalyst could be readily recovered by filtration and used several times without any discernible loss in activity. The hydrogen sulfate anion of the catalytic system was found to play a critical role in enhancing the reaction time and yield of the acetylation reaction.     

Assessing hazelnut allergens by protein- and DNA-based approaches: LC-MS/MS,ELISA and real-time PCR

Hazelnut (Corylus avellana L.) is responsible for a significant part of the allergies related to nuts. Still, it is a very much appreciated nut and as consequence is widely used in all types of processed foods, such as chocolates. Correct food labelling is currently the most effective means of preventing the consumption of allergenic ingredients, namely hazelnut, by the sensitised/allergic individuals. Thus, to verify labelling compliance and to ensure allergic patient protection, the development of highly sensitive methodologies is of extreme importance. In this study, three major methodologies, namely enzyme-linked immunosorbent assays (ELISA), liquid chromatography coupled with mass spectrometry and real-time polymerase chain reaction, were evaluated for their performance regarding the detection of hazelnut allergens in model chocolates. The sandwich ELISA and respective antibodies were in-house developed and produced. With sensitivity levels of approximately 1 mg kg^?1 and limits of quantification of 50–100 mg kg^?1, all the performed methods were considered appropriate for the identification of hazelnut in complex foods such as chocolates. To our knowledge, this was the first successful attempt to develop and compare three independent approaches for the detection of allergens in foods.

A numerical scheme based on Bernoulli wavelets and collocation method for solving fractional partial differential equations with Dirichlet boundary conditions

In this paper, an efficient and accurate numerical method is presented for solving two types of fractional partial differential equations. The fractional derivative is described in the Caputo sense. Our approach is based on Bernoulli wavelets collocation techniques together with the fractional integral operator, described in the Riemann‐Liouville sense. The main characteristic behind this approach is to reduce such problems to those of solving systems of algebraic equations, which greatly simplifies the problem. By using Newton's iterative method, this system is solved and the solution of fractional partial differential equations is achieved. Some results concerning the error analysis are obtained. The validity and applicability of the method are demonstrated by solving four numerical examples. Numerical examples are presented in the form of tables and graphs to make comparisons with the results obtained by other methods and with the exact solutions much easier.     

Dynamic 1H NMR study of the barrier to rotation about the C-N bond in primary carbamates and its solvent dependence

The dynamic 1H NMR study of some primary carbamates in the solvents CDCl3 and CD3COCD3 between 183 and 298 K is reported. The free energies of activation, thus obtained (12.4 to 14.3 kcal mol-1), were attributed to the conformational isomerization about the N-C bond. These barriers to rotation show solvent dependence in contrast to the tertiary analogues and are lower in free energy by ca. 2-3 kcal mol-1.     

Inherent safety evaluation in process plants— a comparison of methodologies

A global population increase and an improved standard of living are generally expected. To meet these demands, an increased production of chemicals will be necessary while protecting human health and the environment. However, most current methods of chemical production are unsustainable. New designs must result in plants that assure process and operator safety, the sustained health of workers and the community, and the protection of the environment. Traditional safety precautions and process controls minimize risk but cannot guarantee the prevention of accidents followed by serious consequences. Therefore, the general approach to environmental and safety problems must be changed from reactive to proactive. One way is to further develop the concept of inherent safety. In this paper some methods for inherent safety evaluations are reviewed. The aim of the study is to analyze the different tools available for inherent safety evaluation and identify the most important criteria in determining the inherent safety of a process plant. A model is proposed to show the interactions of different factors on the inherent safety level of a process and the model is illustrated by a case study.     

A novel efficient numerical method to simulate electrochemical process for a lithium ion battery

The simulation plays an important role in understanding of electrochemical behavior and internal process of lithium ion batteries. The existing finite difference method (FDM) to conduct the simulation of electrochemical process is time-consuming and computationally expensive. In this paper, a novel numerical method is proposed to accelerate the solution of the electrochemical model for a lithium ion battery. It is implemented in three steps. In the first step, physical analogy of electrochemical process to an electric circuit is used to solve charge conservation equations. In the second and third step, control volume method is used to solve species conservation equations. The simulation results show that the proposed method is much faster than the FDM by 2.2 times while maintain high accuracy which is verified by simulation and experimental data as well.     

Sensitive amperometric pyridoxine sensor based on self‐assembled Prussian blue nanoparticle‐modified poly(o‐phenylenediamine)/glassy carbon electrode

Prussian blue nanoparticles (PBNPs) were prepared by a self‐assembly process on a glassy carbon electrode (GCE) modified with poly(o‐phenylenediamine) (PoPD) film. The stepwise fabrication process of PBNP‐modified PoPD/GCE was characterized using scanning electron microscopy and electrochemical impedance spectroscopy. The prepared PBNPs showed an average size of 70 nm and a homogeneous distribution on the surface of the modified electrode. The PBNPs/PoPD/GCE showed electrocatalytic activity towards the oxidation of pyridoxine (PN) and was used as an amperometric sensor. The modified electrode exhibited a linear response for PN oxidation over the concentration range 3–38.5 μM with a detection limit of ca 6.10 × 10^?7 M (S/N = 3) and sensitivity of 2.79936 × 10³ mA M^?1 cm^?2 using an amperometric method. The mechanism and kinetics of the catalytic oxidation reaction of PN were investigated using cyclic voltammetry and chronoamperometry. The values of α, k_cat and D were estimated as 0.36, 1.089 × 10² M^?1 s^?1 and 8.9 × 10^?5 cm² s^?1, respectively. This sensor also exhibited good anti‐interference and selectivity. Copyright © 2016 John Wiley & Sons, Ltd.