Dihydroanthracenone metabolites from the endophytic fungus Diaporthe melonis isolated from Annona squamosa

Chemical investigation of the endophytic fungus Diaporthe melonis, isolated from Annona squamosa, yielded two new dihydroanthracenone atropodiastereomers, diaporthemins A (1) and B (2), together with the known flavomannin-6,6′-di-O-methyl ether (3). The structures of the new compounds were established on the basis of extensive 1D and 2D NMR spectroscopy, as well as by high resolution mass spectrometry and by CD spectroscopy. Compounds 1–3 were tested for their antimicrobial activity against a multi-resistant clinical isolate of Staphylococcus aureus 25697, a susceptible reference strain of S. aureus ATCC 29213 and against Streptococcus pneumoniae ATCC 49619. Compound 3 strongly inhibited S. pneumonia growth with a MIC value of 2 μg/mL, and showed moderate activity against the S. aureus multi-resistant clinical isolate and susceptible reference strain (MIC 32 μg/mL), whereas 1 and 2 were not active against the tested strains.     

Human cerebrovascular contractile receptors are upregulated via a B-Raf/MEK/ERK-sensitive signaling pathway

Background  

Cerebral ischemia results in a rapid increase in contractile cerebrovascular receptors, such as the 5-hydroxytryptamine type 1B (5-HT_1B), angiotensin II type 1 (AT₁), and endothelin type B (ET_B) receptors, in the vessel walls within the ischemic region, which further impairs local blood flow and aggravates tissue damage. This receptor upregulation occurs via activation of the mitogen-activated protein kinase pathway. We therefore hypothesized an important role for B-Raf, the first signaling molecule in the pathway. To test our hypothesis, human cerebral arteries were incubated at 37°C for 48 h in the absence or presence of a B-Raf inhibitor: SB-386023 or SB-590885. Contractile properties were evaluated in a myograph and protein expression of the individual receptors and activated phosphorylated B-Raf (p-B-Raf) was evaluated immunohistochemically.     

Abundance of Invariant and Almost Invariant Pure States of -Dynamical Systems

We show that invariant states of -dynamical systems can be approximated in the weak^*-topology by invariant pure states, or almost invariant pure states, under various circumstances. Received: Received: 25 July 1996 / Accepted: 9 December 1996     

A Dynamic Mixture Model for Unsupervised Tail Estimation without Threshold Selection

Exceedances over high thresholds are often modeled by fitting a generalized Pareto distribution (GPD) on R⁺. It is difficult to select the threshold, above which the GPD assumption is enough solid and enough data is available for inference. We suggest a new dynamically weighted mixture model, where one term of the mixture is the GPD, and the other is a light-tailed density distribution. The weight function varies on R⁺ in such a way that for large values the GPD component is predominant and thus takes the role of threshold selection. The full data set is used for inference on the parameters present in the two component distributions and in the weight function. Maximum likelihood provides estimates with approximate standard deviations. Our approach has been successfully applied to simulated data and to the (previously studied) Danish fire loss data set. We compare the new dynamic mixture method to Dupuis' robust thresholding approach in peaks-over-threshold inference. We discuss robustness with respect to the choice of the light-tailed component and the form of the weight function. We present encouraging simulation results that indicate that the new approach can be useful in unsupervised tail estimation, especially in heavy tailed situations and for small percentiles.     

Effects of thermal annealing on the viscoelastic properties and morphology of bimodal hard/soft latex blends

The effects of thermal annealing on the viscoelastic properties and morphology of films prepared from bimodal latex blends containing equal weight fractions of soft and hard latex particles with controlled sizes were investigated. The thermal and viscoelastic properties of as‐dried and annealed samples were investigated with differential scanning calorimetry and dynamic mechanical analysis (DMA). Throughout the thermal annealing, the latex blend morphologies were also followed with atomic force microscopy and transmission electron microscopy (TEM). A particulate morphology, consisting of hard particles evenly dispersed in a continuous soft phase, was observed in the TEM micrographs of the as‐dried latex blends and resulted in an enhancement of the mechanical film properties at temperatures between the α relaxations of the soft and hard phases in the DMA thermograms. As soon as the thermal annealing involved temperatures higher than the glass‐transition temperature of the hard phase, the hard particles progressively lost their initial spherical shape and formed a more or less continuous phase in the latex blends. This induced coalescence of the hard particles was confirmed by the association of the experimental viscoelastic data with theoretical predictions, based on self‐consistent mechanical models, which were performed by the consideration of either a particulate or cocontinuous morphology for the bimodal latex blends. © 2005 Wiley Periodicals, Inc. J Polym Sci Part B: Polym Phys 43: 2289–2306, 2005     

Structure spaces of approximately finite-dimensional C1-algebras

A necessary and sufficient condition for a topological space Y to be the primitive ideal space of an approximately finite dimensional C¹-algebra is proved. The condition is that Y can be obtained from a second countable, totally disconnected, compact Hausdorff space X by equipping X with a topology τ coarser than the original topology and setting Y = (X, τ)/~. ~ is the equivalence relation on X defined by x ~ y iff all sets $\text{o}$ ? τ that contain x also contain y, and conversely. The topology τ must satisfy some additional requirements.     

Generation of semigroups,and two-dimensional quantum lattice systems

We consider two types of conditions on an operator on a Banach space which ensure that it is the generator of a semigroup of contractions. First, S. Sakai's concept of commutative normal ¹-derivations of UHF algebras is generalized to “approximately commutative operators” on Banach spaces. Next we consider the situation in which the domain of the operator contains an increasing sequence of “approximately invariant subspaces,” and generalize results of A. Kishimoto and P. E. T. Jørgensen. A corollary is the existence of time development for two-dimensional quantum lattice models when the average surface energy per unit surface is uniformly bounded in the volume.     

Poly(methyl methacrylate-co-ethyl acrylate) latex particles with poly(ethylene glycol) grafts: structure and film formation

Water-based copolymer dispersions were prepared using methyl methacrylate (MMA), ethyl acrylate (EA) (MMA/EA = 1:2), and a series of nonionic polymerizable surfactants, i.e., "surfmers" based on poly(ethylene glycol)-(meth)acrylates. The latexes were compared with the behavior of a conventionally stabilized (nonionic nonylphenol ethoxylate, NP100 with 84 ethylene oxide units) dispersion with the same MMA-EA composition (PMMAEA). A number of techniques were employed in order to characterize structure, dynamics, and film formation properties: solution/solid-state NMR, dynamic/static light scattering (DLS/SLS), differential scanning calorimetry (DSC), tensile/shear mode dynamic mechanical thermal analysis (DMTA), and atomic force microscopy (AFM). The surfmers were found to be miscible with the MMA-EA copolymer at room temperature, with 46-85 mol % of the reacted surfmer detected at the particle surfaces, and the remaining part buried in the particle bulk. In contrast, the NP100 surfactant formed a separate interphase between the copolymer particles with no mixing detected at room temperature or at 90 degrees C. For a 4.0% dry weight concentration, NP100 phase separated and further crystallized at room temperature over a period of several months. Composition fluctuations related to a limited blockiness on a length scale above approximately 2 nm were detected for PMMAEA particles, whereas the surfmer particles were found to be homogeneous also below this limit. On a particle-particle level, the dispersions tended to form colloidal crystals unless hindered by a broadened particle size distribution or, in the case of PMMAEA, by the action of NP100. Finally, a surface roughness (Rq) master plot was constructed for data above the glass transition temperature (Tg) from Tg + 11 degrees C to Tg + 57 degrees C and compared with the complex shear modulus over 11 frequency decades. Shift factors from the 2 methods obeyed the same Williams-Landel-Ferry (WLF) temperature dependence, thus connecting the long-time surface flattening process to the rheological behavior of the copolymer.