A Highly Selective and Sensitive Chemiluminescent Probe for Real‐Time Monitoring of Hydrogen Peroxide in Cells and Animals

Selective and sensitive molecular probes for hydrogen peroxide (H₂O₂), which plays diverse roles in oxidative stress and redox signaling, are urgently needed to investigate the physiological and pathological effects of H₂O₂. A lack of reliable tools for in vivo imaging has hampered the development of H₂O₂ mediated therapeutics. By combining a specific tandem Payne/Dakin reaction with a chemiluminescent scaffold, H₂O₂‐CL‐510 was developed as a highly selective and sensitive probe for detection of H₂O₂ both in vitro and in vivo. A rapid 430‐fold enhancement of chemiluminescence was triggered directly by H₂O₂ without any laser excitation. Arsenic trioxide induced oxidative damage in leukemia was successfully detected. In particular, cerebral ischemia‐reperfusion injury‐induced H₂O₂ fluxes were visualized in rat brains using H₂O₂‐CL‐510 , providing a new chemical tool for real‐time monitoring of H₂O₂ dynamics in living animals.     

L''exactitude du rendement des machines agricoles

Precision of the working height, or depth, of earth-moving equipment and other farm machinery can have a marked effect on the quality of the operation. The main factors affecting a machine's performance in maintaining the correct working height are its geometric parameters and the soil surface upon which it operates. The soil surface profile is defined by the “mean slope”, by the “root-mean-square” (RMS) of the deviations from the mean slope, and by the wavelengths calculated by Fourier analysis. The shortest wavelength with an amplitude above 1 cm was used in this study to find the effect of the soil surface waves on the accuracy of farm machinery operations. The machine's geometric parameters affecting its accuracy are its wheelbase and the lengthwise location of the working point (above or under ground) in relation to the wheels. A computer simulation analysis showed that the minimum deviations from the required height are found at the wheels of the machine. The deviations increased at the middle of the machine's span and rose steeply outside the span. The shorter the wheel-span of the machine relative to the wavelengths, the smaller the deviations will be. Smoothed surfaces have longer wavelengths and smaller deviations and as a result, higher accuracy of the working height, or depth, of the machines working on them.     

Single frequency lasing using coherent combining

We show that by coherently combining several solid state lasers it is possible to obtain a single frequency output. This is experimentally demonstrated by coherently combining four Nd:YAG lasers channels, each with a properly chosen cavity length, in order to suppress unwanted longitudinal modes and obtain a single frequency output. We also present a model that accurately predicts and supports out experimental results.     

Integrable models through representations of the hecke algebra

We present new solutions to the Yang-Baxter equation through representations of the Hecke algebra. The generators of the Hecke algebra are considered as Boltzmann weights for face models. The heights live in a graph. These models were conjectured to be integrable by Di Francesco and Zuber. We prove integrability for some of the suggested models by building explicitly the Boltzmann weights. Some relations that the Boltzmann weights satisfy and the consequence on partition functions with various boundary conditions are also discussed.     

Origin of the binary pulsar J0737-3039B

Evolutionary scenarios suggest that the progenitor of the new binary pulsar J0737-3039B was a He star with M>(2.1-2.3)M. . We show that this case implies that the binary must have a large (>120 km/s) center of mass velocity. However, the location, approximately 50 pc from the Galactic plane, suggests that the system has, at high likelihood, a significantly smaller center of mass velocity and a progenitor more massive than 2.1M. is ruled out (at 97% C.L.). A progenitor mass around 1.45M. , involving a new previously unseen gravitational collapse, is kinematically favored. The low mass progenitor is consistent with the recent scintillation based velocity measurement of 66+/-15 km/s and rules out the high mass solution at 99% C.L. Conversely, if the unlikely higher mass solution is the true one we should increase the estimated rate of neutron star mergers by a factor of at least 2.     

Baryogenesis from the Kobayashi-Maskawa phase

The smallness of quark masses suppresses the CP violation from the Kobayashi-Maskawa phase to a level that is many orders of magnitude below what is required to explain the observed baryon asymmetry. We point out that if, as a result of time variation in the Yukawa couplings, quark masses were large at the time of the electroweak phase transition, then the Kobayashi-Maskawa mechanism could be the source of the asymmetry. The Froggatt-Nielsen mechanism provides a plausible framework where the Yukawa couplings could all be of order 1 at that time, and settle to their present values before nucleo-synthesis. The problems related to a strong first order electroweak phase transition may also be alleviated in this framework. Our scenario reveals a loophole in the commonly held view that the Kobayashi-Maskawa mechanism cannot be the dominant source of CP violation to play a role in baryogenesis.     

Decoupling of Proper Transfer Functions

This paper studies the state space and feedback aspects of linear system decoupling. Given a minimal realization for a proper transfer function W (s), a general procedure is given for the parametrization of all the minimal decouplings of W (s) into two proper subsystems. This completes and unifies known results on factorization and cascade decomposition.     

High-numerical-aperture focusing of radially polarized doughnut beams with a parabolic mirror and a flat diffractive lens

Recently, Dorn et al. [Phys. Rev. Lett. 91, 233901 (2003)] demonstrated the significance of radially polarized doughnut beams in obtaining very small focal spots (with an area of approximately 0.26 lambda2) with high-numerical-aperture (NA) aplanatic microscope objectives. We propose two simple alternative ways to focus such radially polarized beams: a parabolic mirror and a flat diffractive lens. Because of their large apodization factor for a high NA, a significant further reduction in spot area (up to a factor of 1.76 at a NA of 1) compared with the aplanatic system can be achieved.     

A three dimensional dark focal spot uniformly surrounded by light

A new technique is proposed for generating a tight dark focal spot surrounded by uniform light intensity in all directions. It is based on a single focusing lens illuminated from one side, hence the alignment sensitivities associated with 4π methods are eliminated. Such a beam can be useful, e.g. as a dark atomic trap, and as the erase beam in three dimensional super-resolution fluorescence microscopy.     

Surface properties of plasma membrane vesicles isolated from melon (Cucumus melo L.) root cells differing in salinity tolerance

The hypotheses that genotypic differences in salinity tolerance may result from (i) differences in global surface charge density or (ii) from differences in global Ca²⁺ binding were tested. An attempt was made to correlate the differing salinity tolerance of four melon cultivars with surface properties of vesicles extracted from the plasma membrane (PM) of their root cells. Surface characterization involved measurements of electrophoretic mobility and sorption of ⁴⁵Ca²⁺ to the vesicles in the presence of varying concentrations of Ca²⁺, Na⁺ and Mg²⁺. Irrespective of salinity tolerance, vesicles from the four cultivars yielded similar ζ potentials under similar conditions, indicating similar global surface charge densities. Sorption studies with vesicles from two cultivars differing in salinity tolerance predicted independently this result of equal surface charge density. The estimated global binding affinities of Ca²⁺, Na⁺ and Mg²⁺ to the PM of both cultivars were the same with binding coefficients of 50, 0.8 and 9 M⁻¹, respectively. Consequently, the hypotheses enumerated above to interpret genotypic differences in salinity toxicity are rejected. However, vesicles from the salt-resistant strain sorbed 19% more Ca²⁺ per given amount of protein in the membrane, indicating the existence of a larger number of negatively charged surface sites per given amount of protein and a smaller amount of protein per given area of membrane. Genotypic differences in site-specific Ca²⁺-binding affinity (e.g. at ion channels) remain a viable hypothesis for genotypic differences in salinity tolerance.