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61.
Ubavka B. Mio
Milena Petkovi Milorad Davidovi Miljenko Peri Tyno Abdul-Redah 《Journal of Molecular Structure》2008,885(1-3):131-138
Systematic experimental investigations of heteropoly compounds, particularly their structure and activity, led to the conclusion that most of their characteristics are governed by the presence of protons and protonic entities. Special attention has been paid to two forms of 12-tungstophosphoric acid: hexahydrate (WPA-6) and dehydrated phase (WPA-0). It was postulated that in WPA-6 dynamic equilibrium of protonic entities exists, and that dehydrated phase is stabilized by protons. To confirm the role of the “free protons” or “proton gas” derived on the basis of thermal, structural and spectroscopic experimental studies, we carried out also ab initio calculations on a number of systems containing ion. We were not able to perform direct calculations on the real systems investigated experimentally since the structure of heteropoly compounds is too complex. However, it has been found that ion in WPA-6 definitely is not planar and the results obtained indirectly support the postulated dynamic equilibrium, i.e. possibility of existing of free protons. 相似文献
62.
Caetano P. Sabino Martha S. Ribeiro Mark Wainwright Carolina dos Anjos Fábio P. Sellera Milena Dropa Nathalia B. Nunes Guilherme T. P. Brancini Gilberto U. L. Braga Victor E. Arana-Chavez Raul O. Freitas Nilton Lincopan Maurício S. Baptista 《Photochemistry and photobiology》2023,99(2):742-750
The unbridled dissemination of multidrug-resistant pathogens is a major threat to global health and urgently demands novel therapeutic alternatives. Antimicrobial photodynamic therapy (aPDT) has been developed as a promising approach to treat localized infections regardless of drug resistance profile or taxonomy. Even though this technique has been known for more than a century, discussions and speculations regarding the biochemical mechanisms of microbial inactivation have never reached a consensus on what is the primary cause of cell death. Since photochemically generated oxidants promote ubiquitous reactions with various biomolecules, researchers simply assumed that all cellular structures are equally damaged. In this study, biochemical, molecular, biological and advanced microscopy techniques were employed to investigate whether protein, membrane or DNA damage correlates better with dose-dependent microbial inactivation kinetics. We showed that although mild membrane permeabilization and late DNA damage occur, no correlation with inactivation kinetics was found. On the other hand, protein degradation was analyzed by three different methods and showed a dose-dependent trend that matches microbial inactivation kinetics. Our results provide a deeper mechanistic understanding of aPDT that can guide the scientific community toward the development of optimized photosensitizing drugs and also rationally propose synergistic combinations with antimicrobial chemotherapy. 相似文献
63.
The thermal and thermo-oxidative processes in Aroniamelanocarpa (black chokeberry) were investigated using combined thermo-analytical (TG-DTA) and spectroscopic (FTIR) experimental techniques. Isoconversional analysis revealed that the process in an inert (argon) atmosphere was probably governed by chlorogenic acid degradation, where autocatalysis (described by the empirical ?esták-Berggren model) might occur due to water already present in the early stages of the process through hydrolysis. Thermal degradation is described by the intrinsic kinetic parameters, where the degradation rate increases proportionally with an increase in the heating rate. Under oxidative conditions, the process was found to be primarily driven by neochlorogenic acid degradation. The thermo-oxidative degradation of Aroniamelanocarpa fresh samples can be described by two competitive reactions, where it was established that a cyanidin-3-glucosylrutinoside degradation made a significant contribution to a comprehensive kinetics. This study showed the targeting of the neochlorogenic acid in Aroniamelanocarpa fresh samples to have a strong hydrogen-donating activity, thereby rendering it capable of very efficiently entrapping the peroxy radicals. Current research has demonstrated that the relative contribution of the two competitive reactions to the overall process is highly dependent on the heating rate of the system under consideration. 相似文献
64.
Davis WC Christopher SJ Pugh RS Donard OF Krupp EA Point D Horvat M Gibicar D Kljakovic-Gaspic Z Porter BJ Schantz MM 《Analytical and bioanalytical chemistry》2007,387(7):2335-2341
This paper describes the development of two independent analytical methods for the extraction and quantification of methylmercury
from marine biota. The procedures involve microwave extraction, followed by derivatization and either headspace solid-phase
microextraction (SPME) with a polydimethylsiloxane (PDMS)-coated silica fiber or back-extraction into iso-octane. The identification
and quantification of the extracted compounds is carried out by capillary gas chromatography/mass spectrometric (GC/MS) and
inductively coupled plasma mass spectrometric (GC/ICP-MS) detection. Both methods were validated for the determination of
methylmercury (MeHg) concentrations in a variety of biological standard reference materials (SRMs) including fresh-frozen
tissue homogenates of SRM 1946 Lake Superior fish tissue and SRM 1974a organics in mussel tissue (Mytilus edulis) and then applied to the certification effort of SRM 1947 Lake Michigan fish tissue and SRM 1974b organics in mussel tissue
(Mytilus edulis). While past certifications of methylmercury in tissue SRMs have been based on two independent methods from the National
Institute of Standards and Technology (NIST) and participating laboratories, the methods described within provide improved
protocols and will allow future certification efforts to be based on at least two independent analytical methods within NIST. 相似文献
65.
Djurdjević P Joksović L Jelić R Djurdjević A Stankov MJ 《Chemical & pharmaceutical bulletin》2007,55(12):1689-1699
Complex formation between aluminum(III) ion and fluoroquinolone antibacterials-either moxifloxacin (4th generation antibiotic) or fleroxacin (2nd generation antibiotic) were studied in aqueous solutions without and in the presence of sodium dodecylsulfate (SDS). The investigations were performed by glass electrode potentiometric (ionic medium: 0.1 mol/dm(3) LiCl, 298 K), UV spectrophotometric, multinuclear (1H and 13C) magnetic resonance and ESI-MS measurements. The experimental data were consistent with the formation of Al(HL)L2+, Al(HL)3+ AlL2+, Al(OH)L+ and Al(OH)2L complexes in the pH interval ca. 3-8 and up to 5 : 1 ligand to metal mole ratio with range of Al3+ concentrations between ca. 0.025 to 1.0 mmol/dm3. The binary complex, AlL2+ is fairly stable (log beta(1,0,1) ca. 11.0) and its stability increases in the presence of SDS. At higher concentration ratios of ligands to aluminum, up to 5 : 1, the complex Al(HL)L2+ is formed with rather high overall stability constant (log beta(1,1,2) ca. 24.0). The ESI-MS data generally, confirmed the derived model, and the formation of the complex with ligand to metal ratio 2 : 1. NMR measurements indicate that both ligands utilize 4-carbonyl and carboxyl oxygens as donor atoms. The presence of surface active substance, SDS, favors the formation of the complex in which the ligand is protonated, i.e. Al(HL) and its maximum formation is shifted toward milder acidic region (pH ca. 4). The aluminum-quinolone complexes may affect the bio-distribution of both, quinolone and/or aluminum ion upon concomitant ingestion of aluminum-based antacids or phosphate binders and fluoroquinolones. 相似文献
66.
Pesticides are widely utilized at various stages of cultivation and during postharvest storage to protect plants against a range of pests and/or to provide quality preservation. Reliable confirmatory methods are required to monitor pesticide residues in baby foods and to ensure the safety of baby food supply. This review covers methods in which pesticide residues have been determined in baby food by the use of a wide range of chromatographic techniques after various sample preparation steps. The main attention is paid to the evaluation and improvement of sample extraction and clean-up methods (liquid extraction, solid-phase extraction (SPE), dispersive SPE (DSPE), microextraction procedures, matrix solid-phase dispersion (MSPD) and supercritical fluid extraction (SFE)) considering low concentration levels of pesticide residues in baby food resulting from stringent European Union (EU) legislation. Instrumental aspects together with the matrix effects significantly contributing to the most important parameters considered in pesticide residues analysis of baby food--limits of detection (LODs) and limits of quantification (LOQs) were included within the scope of this overview. Paper involves also monitoring studies. 相似文献
67.
David J. Strike Andreas Hengstenberg Maurizio Quinto Christian Kurzawa Milena Koudelka-Hep Wolfgang Schuhmann 《Mikrochimica acta》1999,131(1-2):47-55
An investigation of an array of four Pt microband electrodes 25 μm wide and spaced 25 μm apart was performed with the scanning
electrochemical microscope (SECM). Where possible the SECM measurements were confirmed with conventional electrochemical measurements.
It is shown how the sensiti- vity of the SECM recycling current to the activity of the underlying surface can be used to probe
the homogeneity of enzyme-modified microelectrodes. The diffusion of H2O2 between these micro enzyme- electrodes and unmodified electrodes was investigated and it was demonstrated how the SECM can
be a powerful tool in the elucidation of the properties of these electrodes.
Received June 8, 1998. Revision November 12, 1998. 相似文献
68.
Hybrid organic-inorganic coatings and free-standing films made from [3-(glycidyloxy)propyl]trimethoxysilane, amino-terminated poly(oxypropylene) (Jeffamine D-230), colloidal polyaniline nanoparticles and, in some cases, colloidal nanosilica were prepared and characterized. HCl or 4-methylbenzene-1-sulfonic acid were used as catalysts for the sol-gel process and pH tuning, water-propan-2-ol mixture as a solvent. Electrical and mechanical properties and surface morphology of films were studied. The coatings were blue and non-conducting, or green and conducting, depending on preparation conditions. They have a smoother surface than in-situ polymerized polyaniline films. 相似文献
69.
Sergio Banfi Manuela Bergna Milena Povolo Giovanna Contarini 《Journal of separation science》1999,22(2):93-96
The PTV (Programmable Temperature Vaporizer) is a split/splitless injector which allows the sample to be introduced at a relatively low temperature, thus affording accurate and reproducible sampling. After injection the PTV is rapidly heated to transfer the vaporized components into the capillary column. This type of injector has proved to be an efficient tool for the evaluation of fatty acids, essential oils, and pesticides in food analysis. In this work the suitability of PTV for the analysis of milk fat purity by the Official EU method was evaluated. This method is based on the gas chromatographic determination of triglycerides only according to their total number of carbon atoms followed by the application of formulae deriving from multiple linear regressions. The analysis is currently carried out with a packed column or a short capillary column and an on-column injection system. Several samples of pure milk fat and mixtures of milk fat with foreign fat were analyzed with the same capillary column and by using both PTV and on-column injection systems. The results show that the gas chromatographic profile obtained by PTV is comparable with that obtained by the on-column injector, while repeatability and reproducibility data meet the requirements indicated in the Official Method. Therefore, this study demonstrates that it is possible to use the PTV instead of the on-column injector to determine the purity of milk fat with this method. 相似文献
70.
Milena Povolo Eliana Bonfitto Giovanna Contarini Piero M. Toppino Alberto Daghetta 《Journal of separation science》1999,22(2):97-102
The adulteration of milk fat with foreign fat has been and still is a major concern in the dairy industry. Milk fat purity is currently evaluated by triglyceride analysis by using the Official EU method. The detection limit of the various vegetable and animal fats ranges between 4 and 6%. This research was carried out to verify whether it is possible to decrease the detection limits of beef tallow, which is the most widely used adulterating animal fat. For this purpose, determinations of diglycerides and 3,5-cholestadiene, together with the Official EU method, were applied both to several samples of pure milk fat and to mixtures of milk fat with different percentages of beef tallow. The best results were obtained combining the data deriving from the three determinations by multivariate statistical techniques; in particular, the statistical model obtained by the UNEQ technique seems to be able to decrease the detection limit of beef tallow from 5.2 to 2%. The diglyceride and 3,5-cholestadiene evaluation, combined with the Official EU method for triglycerides, can be usefully applied both to detect small additions of beef tallow and to demonstrate the adulteration of milk fat samples showing results close to the detection limit of the official method. 相似文献