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排序方式: 共有219条查询结果,搜索用时 31 毫秒
141.
Masayasu Mie Tetsuro Kai Thao Le Anthony E. G. Cass Eiry Kobatake 《Applied biochemistry and biotechnology》2013,169(1):250-255
Aptamers are single-strand oligonucleotides that are generated by the systemic evolution of ligands by exponential enrichment (SELEX) technique and that can bind to target molecules specifically. However, only a few aptamers have been developed to date against tumor markers. To utilize aptamers for tumor diagnosis, a variety of aptamers are required. Here, a single-stranded DNA aptamer specific for pro-gastrin-releasing peptide (proGRP), a marker for small cell lung cancer, was selected using SELEX. After selection, identical sequences were found in the DNA library. This sequence was selected and its binding affinity to proGRP was evaluated using surface plasmon resonance. 相似文献
142.
Dr. Mie Højer Vilhelmsen Prof. Dr. A. Stephen K. Hashmi 《Chemistry (Weinheim an der Bergstrasse, Germany)》2014,20(7):1901-1908
A wide range of gold‐catalyzed reactions based on a dual activation mechanism has recently been reported in the literature. Herein, we present a computational investigation of the mechanism for the formation of dibenzopentalenes from 1‐ethynyl‐2‐(phenylethynyl)benzene. Transition states have been found, which substantiate the dual activation mechanism previously published and furthermore point towards a continuous presence of two gold moieties throughout the mechanistic cycle, an observation of high importance for all reactions in the field of dual activation. The initial activation of the diyne has been shown to proceed via an intermolecular transfer of a cationic gold catalyst from the thermodynamically preferred geminal‐σ,π‐acetylide complex to the active non‐geminal analogue. Furthermore, the regioselectivity of a 5‐endo versus a 6‐endo cyclization has been addressed, and the 5‐endo cyclization was found to be most favorable both thermodynamically and with regard to the activation barrier. 相似文献
143.
Junta Yamamichi Tetsunori Ojima Mie Iida Kimiko Yurugi Takeshi Imamura Eishi Ashihara Shinya Kimura Taira Maekawa 《Analytical and bioanalytical chemistry》2014,406(18):4527-4533
In clinical settings, serum antibody levels serve as markers of pathology. For example, antibodies related to autoimmune diseases are among the conventional targets in laboratory tests. Simple clinical tests can improve the efficacy of laboratory practice. This study describes a single-step, wash-free technique for optically detecting antibodies in human serum through the localized surface plasmon resonance (LSPR) of gold nanoparticles. As a proof-of-concept experiment, the amount of antibiotin dissolved in human serum was measured with a LSPR-based biosensor in a wash-free manner using a conventional 96-well microtiter plate and a plate reader. For an efficient surface modification of biosensors, zwitterionic copolymer was used as a scaffold on the gold nanoparticle surface to immobilize antigen and blocking reagent. Single-step, wash-free measurement of antibiotin in human serum was successfully achieved. In addition, nonspecific responses from serum contents were significantly reduced because both the copolymer and hydrophilic antigen reagent that we employed were composed of poly(ethylene oxide) spacer. Comparative experiments of the antigen-antibody reaction in serum to that in buffered solution revealed that serum is a favorable environment for the biological reaction. In conclusion, our gold-nanoparticle-based LSPR method may provide a rapid and simple way to measure the amount of antibody in serum quantitatively in clinical practice. Figure
Experimental setups illustrating two different methods of surface modification for the gold nanoparticles: biotinylated BSA and zwitterionic copolymer. The amount of antibiotin that attached to biotin on the surface was measured by the peak shift of LSPR spectra using a 96-well microtiter plate with immobilized gold nanoparticles 相似文献
144.
A. Stock G. Mie K. Arndt B. Bavink F. Winter S. Herzog und Ferdinand Henrich 《Fresenius' Journal of Analytical Chemistry》1920,59(1):16-17
Ohne Zusammenfassung 相似文献
145.
146.
147.
Sir William Ramsay John B. C. Kershaw P. Ferchland W. Herz Ostwald G. Mie und A. Seidell 《Fresenius' Journal of Analytical Chemistry》1908,47(4):240-242
Ohne Zusammenfassung 相似文献
148.
Mingdeng Wei Kewei Wei M. Ichihara Haoshen Zhou 《Electrochemistry communications》2008,10(8):1164-1167
In this study, H2Ti3O7 nanowires were successfully synthesized via a hydrothermal process and post-treatments. The diameter of the nanowires is found to be about 30 nm and the length up to several micrometers. A lithium battery using H2Ti3O7 nanowires as the active material of the positive electrode exhibits a discharge capacity of 100 mA hg−1 and still keeps stable after 200 cycles at a current density as high as 40 Ag−1, demonstrating excellent high rate performance. 相似文献
149.
Akitami Ichihara Yasuji Nakamura Hirokazu Kawagishi Sadao Sakamura 《Tetrahedron letters》1979,20(39):3735-3736
The absolute configuration of lappaol A and F was determined by the correlation of the ozonolysis products with 2R,3R-(?)-dicarbomethoxymethyl-γ-butyrolactone and S-(?)-methyl paraconate. 相似文献
150.