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61.
In this paper, a novel gold nanoparticles based protein immobilization method was designed. Biocomposites of gold nanoparticles and proteins were successfully coated on poly(methyl methacrylate) (PMMA) plates and polystyrene microtiter plates. The proteins could be immobilized on solid materials with high density and better bioactivity. Based on above design, chemiluminescence (CL) imaging assay for determination of H2O2 and recombinant human interleukin-6 (rHu IL-6) was developed. The linear range and the loading capability were greatly improved when compared with imaging assay performed with direct proteins immobilization. Under the selected experimental conditions, a linear relationship was obtained between the CL intensity and the concentration of H2O2 in the range of 1.0 × 10−6 to 1.0 × 10−4 mol L−1, and rHu IL-6 in the range of 2.0-312.0 pg mL−1. The detection limits were 2 × 10−7 mol L−1 (3σ) for H2O2 and 0.5 pg mL−1 for rHu IL-6 with relative standard deviation of 3.8% for 3.0 × 10−5 mol L−1 H2O2, and 4.4% for 39.0 pg mL−1 rHu IL-6. This method has been applied to the determination of rHu IL-6 in human serum with satisfactory results. 相似文献
62.
Positively charged gold nanoparticle (positively charged nano-Au), which was prepared, characterized by ξ-potential and transmission electron microscopy (TEM) was used in combination with l-cysteine to fabricate a modified electrode for electrocatalytic reaction of biomolecules. Compared with electrodes modified by negatively charged gold nanoparticle/l-cysteine, or l-cysteine alone, the electrode modified by the positively charged gold nanoparticle/l-cysteine exhibited excellent electrochemical behavior toward the oxidation of biomolecules such as ascorbic acid, dopamine and hydrogen peroxide. Moreover, the proposed mechanism for electrocatalytic response of positively charged gold nanoparticle was discussed. The immunosensor showed a specific to ascorbic acid in the range 5.1 × 10−7-6.7 × 10−4 M and a low detection limit of 1.5 × 10−7 M. The experimental results demonstrate that positively charged gold nanoparticle have more efficient electrocatalytic reaction than negatively charged gold nanoparticle, which opens up new approach for fabricating sensor. 相似文献
63.
For 2 γ min{4, n}, we consider the focusing Hartree equation iu_t+ △u +(|x|~(-γ)* |u|~2)u = 0, x ∈ R~n.(0.1)Let M [u] and E [u] denote the mass and energy, respectively, of a solution u, and Q be the ground state of-△ Q + Q =(|x|~(-γ)* |Q|~2)Q. Guo and Wang [Z. Angew. Math.Phy.,2014] established a dichotomy for scattering versus blow-up for the Cauchy problem of(0.1) if M [u]~(1-s_c)E [u]~(s_c) M [Q]~(1-s_c)E [Q]~(s_c)(s_c=(γ-2)/2). In this paper, we consider the complementary case M [u]~(1-s_c)E [u]~(s_c)≥ M [Q]~(1-s_c)E [Q]~(s_c) and obtain a criteria on blow-up and global existence for the Hartree equation(0.1). 相似文献
64.
Dalbergia odorifera contains high concentrations of flavonoid aglycones and trace flavonoid glycosides. In this study, trace flavonoid glycosides were separated from D. odorifera by titania with matrix solid-phase dispersion (MSPD). Before the MSPD experiment, four standards, including two isoflavone glycosides (genistin and formononetin-8-C-apiosyl (1-6)-glucoside) and their aglycones (genistein and formononetin), were used to compare their retention on a titania column. The effect of acetonitrile concentration and pH on their retention was investigated and a conclusion was drawn that high acetonitrile concentration and pH lead to the greatest difference in the retention of flavonoid as glycosides and aglycones. Besides hydrophilic interaction and ligand-exchange interaction may exist between sugar moiety of flavonoid glycoside and titania, so that flavonoid glycosides have stronger retention than that of aglycones. Based on the chromatographic rule of flavonoid as glycosides and aglycones on the titania column, the MSPD method was optimized to elute high concentration flavonoid aglycones first with 90% acetonitrile and 10% water containing 100 mM ammonium acetate buffer, and then to elute trace flavonoid glycosides with 20% acetonitrile and 80% water containing 1% trifluoroacetate (TFA). Isolated flavonoid glycosides were further analyzed by UPLC-MS/MS, and their fragmentation in MS(2) showed they are C-glycosyl flavonoids. 相似文献
65.
We report here a folic acid conjugated graphene-hemin composite for selective, quantitative and fast colorimetric detection of cancer cells based on the peroxidase-like activity. 相似文献
66.
The potential of using nano-sized aragonite mollusk shell (nano-Bio-ARA) to remove Cd(2+) from contaminated water was investigated by comparing the sorption kinetics and isotherms with the nano-sized calcite-type mollusk shell (nano-Bio-CAL) and nano-sized geological calcite (nano-Geo-CAL). Nano-Bio-ARA displayed extremely high sorption capacity to Cd(2+) (8.91mmol/g), much higher than nano-Bio/Geo-CAL, and many other natural or engineered materials. The results of thermodynamic experiments indicated that the sorption of Cd(2+) on the nano-ARA was a spontaneous and endothermic process. The coexisting metals in the solution displayed competition effect to the sorption of Cd(2+) on nano-Bio-ARA in the following order: Cu(2+)>Cr(3+)>Pb(2+)>Zn(2+)>Ca(2+). EDTA impeded the sorption of Cd(2+) on nano-Bio-ARA due to its strong chelating capacity to Cd(2+) in the solution. The results demonstrate that nano-Bio-ARA is a potential high-effective material to treat Cd(2+) contaminated water. 相似文献
67.
Feng L Zhao C Xiao Y Wu L Ren J Qu X 《Chemical communications (Cambridge, England)》2012,48(55):6900-6902
Here we report a signal-on three-way junction based-electrochemical DNA sensor for distinguishing chiral metallo-supramolecular complexes with an enantioselective recognition ratio of about 3.5. This platform is easy to fabricate, simple to operate and readily regenerated. 相似文献
68.
69.
铜-1-(2-吡啶偶氮)-2-萘酚体系的极谱吸附催化波研究及应用 总被引:3,自引:0,他引:3
在KH2PO4NaOH(pH=6.4)缓冲溶液中,Cu(Ⅱ)1(2吡啶偶氮)2萘酚(PAN)在-0.23V(vs.SCE)产生灵敏的络合吸附波。加入了过硫酸钾以后,波高进一步增加,二阶导数峰电流与1.5×10-8~6.0×10-6mol/LCu2+呈良好的线性关系;检出限为8.0×10-9mol/L。研究了极谱波的性质及增敏的机理,表明该极谱波为吸附催化波。用于大米、绿豆和人发中铜含量的测定,取得了满意的结果。 相似文献
70.
一维光子晶体的双通道位置设计及调整 总被引:25,自引:2,他引:23
通过对法布里-珀罗滤光片性质的研究,以及对法布里-珀罗滤光片中缺陷层厚度变化对通道数目、位置影响的分析,为双通道一维光子晶体的设计提出了一条新的思路。在法布里-珀罗结构的基础上引入一维光子晶体的双对称结构,并通过改变双对称结构的三个缺陷层厚度来实现对禁带内两个通道系列位置的独立调整。设计过程中采用有效界面法对通道的位置进行初步的计算,并通过计算机理论模拟对膜层进行修正,使通道的位置满足设计要求。设计结果表明,通过调整两个厚度参量可以克服双通道一维光子晶体中的通道干涉现象,从而实现通道位置的独立调整。 相似文献