Standardisierung der dünnschichtchromatographischen Trennungen von Flechtensäuren zur Chemotaxonomie von Flechten

Zusammenfassung Die dünnschichtchromatographische Trennung von Flechtenextrakten zur Unterstützung der Taxonomie wurde in Bezug auf die Trennleistung und auf die Reproduzierbarkeit optimiert. Dabei wurden besonders gute Trennleistungen erhalten mit dem Flie?mittelgemisch Benzol:Dioxa:Eisessig=90∶25∶4 bei Verwendung von Kieselgel, dessen Aktivit?t durch eine Umgebung mit relativer Feuchte von 70% festgelegt wurde, und mit Methylenchlorid als Eluiermittel bei Verwendung von mit Oxals?ure impr?gniertem Kieselgel und antiparallelem Aktivit?tsgradienten. Eine hohe Reproduzierbarkeit der Trennungen wurde erreicht durch Verwendung der Vario-KS-Kammer nach Geiss, die die Einstellung einer definierten Schichtaktivit?t bzw. eines definierten Aktivit?tsgradienten und eine reproduzierbare Vorbedampfung der Schicht mit Flie?mittel erm?glicht. Es konnte gezeigt werden, da? durch unterschiedliche chromatographische Trennungen der gleichen Extrakte und durch die Verwendung verschiedener Anf?rbereagenzien die Identifizierung und damit die Aussagekraft für taxonomische Probleme steigt.

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Standardisation of the thin-layer chromatographic separation of lichen acids for the chemotaxonomy of lichens

Summary The thin-layer chromatography of lichen extracts for taxonomic studies has been optimized in relation to separation and to reproducibility. Especially good separations were found with benzene:dioxane:acetic acid (90∶25∶4) developer and silica gel thin-layer plates, whose activity was fixed by an ambient moisture content of 70%, and with methylene chloride developer and silica gel plates impregnated with oxalic acid and antiparallel activity gradient. High reproducibility of separation was obtained using the Geiss Vario-KS tank, which allows the activity of the plates or the activity gradient to be adjusted and which also allows reproducible exposure of plates to the vapour of developing solvents. By different chromatographic separations of the same extracts and by using different colour reactions the accuracy of identification for taxonomic studies has been improved.

     

One-dimensional magnetism and crystal structure ofcatena-di-bromobis(3,5-dimethylpyridine)copper(II)

Summary Crystals ofcatena-di--bromobis(3,5-dimethylpyridine)copper(II) are monoclinic, space group P2₁/a. The unit cell constants area=13.900(2),b=14.416(2),c=4.097(1) Å,=93.49(2)°, V=819.4 ų and Z=2. The structure was determined from powder data using a Guinier-Johansson focussing powder camera. The structure was solved using a simplex method for function minimization to a conventional R-value of 0.13.The structure consists of infinite linear chains parallel toc in which the copper coordination is distorted elongated octahedral. Cu-Br distances were found to be 2.449(7) and 3.286(7) Å, whereas the Cu-N bond length is 2.02(2) Å. All distances are in the range usually observed for this type of compounds.The antiferromagnetic superexchange interactions between adjacent Cu^II ions (J = –21 cm^–1) has been compared with those observed in structural similar CuBr₂L₂ compounds. The differences in observed J-values are discussed briefly, in relation to the structural variations. It appears that very small changes in structural parameters strongly affect the magnetic exchange.     

Spectral Theory of Pauli–Fierz Operators

We study spectral properties of Pauli–Fierz operators which are commonly used to describe the interaction of a small quantum system with a bosonic free field. We give precise estimates of the location and multiplicity of the singular spectrum of such operators. Applications of these estimates, which will be discussed elsewhere, concern spectral and ergodic theory of non-relativistic QED. Our proof has two ingredients: the Feshbach method, which is developed in an abstract framework, and Mourre theory applied to the operator restricted to the sector orthogonal to the vacuum.     

Monitoring and fast detection of mycotoxin-producing fungi based on headspace solid-phase microextraction and headspace sorptive extraction of the volatile metabolites

Solid phase microextraction in combination with capillary GC-MS was used as monitoring technique for the collection and detection of the fungal volatile metabolite (+)-aristolochene by sporulated surface cultures of Penicillium roqueforti. A comparison was made between different toxigenic and nontoxigenic strains of P. roqueforti. Different growth conditions and media, such as malt extract agar, potato dextrose agar and sabouraud dextrose agar were compared. Whereas toxigenic strains produced large amounts of (+)-aristolochene, beta-elemene, valencene and germacrene A, nontoxigenic P. roqueforti strains showed a remarkably different headspace profile, in which ethyl-2-hexenoate, E-beta-caryophyllene, aromadendrene and beta-patchoulene were the predominant volatiles, apart from other sesquiterpene hydrocarbons present at lower concentrations. Stir bar sorptive extraction, was also applied in the headspace sampling mode, i.e. headspace sorptive extraction (HSSE) for the enrichment of fungal volatiles from sporulated surface cultures to differentiate between toxigenic and nontoxigenic fungi. Hence, it can be concluded that headspace analysis of volatile fungal metabolites by SPME and HSSE in combination with capillary GC-MS is a suitable monitoring technique for the fast detection of mycotoxin producing fungi.     

A family of macrocyclic antibiotics with a mixed peptide-peptoid beta-hairpin backbone conformation

Macrocyclic peptidomimetics having a mixed peptide-peptoid backbone have been synthesized and shown to possess antibiotic activity against gram-positive and -negative bacteria with a low hemolytic activity against human erythrocytes; one is shown to adopt a regular beta-hairpin conformation by NMR in aqueous solution.     

Non-covalent stabilization of a beta-hairpin peptide into liposomes

An oligopeptide modified on both the N- and C-termini with hydrophobic moieties was prepared on a solid phase and anchored into a liposome, stabilizing the fold of the peptide into a beta-hairpin, which would otherwise be a random coil.