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Robert Haller 《Colloid and polymer science》1943,105(2):147-149
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Oxidized low-density lipoproteins (OxLDLs) like malondialdehyde-modified low-density lipoprotein (MDA-LDL) play a major role in atherosclerosis and have been proposed as useful biomarkers for oxidative stress. In this study, gold-nanoparticles (GNPs) were functionalized via distinct chemistries with anti-MDA-LDL antibodies (Abs) for selective recognition and capture of MDA-LDL from biological matrices. The study focused on optimization of binding affinities and saturation capacities of the antiMDA-LDL-Ab-GNP bioconjugate by exploring distinct random and oriented immobilization approaches, such as (i) direct adsorptive attachment of Abs on the GNP surface, (ii) covalent bonding by amide coupling of Abs to carboxy-terminated-pegylated GNPs, (iii) oriented immobilization via oxidized carbohydrate moiety of the Ab on hydrazide-derivatized GNPs and (iv) cysteine-tagged protein A (cProtA)-bonded GNPs. Depending on immobilization chemistry, up to 3 antibodies per GNP could be immobilized as determined by ELISA. The highest binding capacity was achieved with the GNP-cProtA-Ab bioconjugate which yielded a saturation capacity of 2.24 ± 0.04 μg mL−1 GNP suspension for MDA-LDL with an affinity Kd of 5.25 ± 0.11 × 10−10 M. The GNP-cProtA-antiMDA-LDL bioconjugate revealed high specificity for MDA-LDL over copper(II)-oxidized LDL as well as native human LDL. This clearly demonstrates the usefulness of the new GNP-Ab bioconjugates for specific extraction of MDA-LDL from plasma samples as biomarkers of oxidative stress. Their combination as specific immunoextraction nanomaterials with analysis by LC–MS/MS allows sensitive and selective detection of MDA-LDL in complex samples. 相似文献
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A. Alessandrello C. Arnaboldi F. T. Avignone III J. Beeman M. Barucci M. Balata C. Brofferio C. Bucci S. Cebrian R. J. Creswick S. Capelli L. Carbone O. Cremonesi A. de Ward E. Fiorini H. A. Farach G. Frossati A. Giuliani D. Giugni E. E. Haller I. G. Irastorza R. J. McDonald A. Morales E. B. Norman P. Negri A. Nucciotti M. Pedretti C. Pobes V. Palmieri M. Pavan G. Pessina S. Pirro E. Previtali C. Rosenfeld A. R. Smith M. Sisti G. Ventura M. Vanzini L. Zanotti 《Physics of Atomic Nuclei》2003,66(3):452-457
CUORE is a proposed tightly packed array of 1000 TeO2 bolometers, each being a cube 5 cm on a side with a mass of 750 g. The array consists of 25 vertical towers, arranged in a square of 5 towers by 5 towers, each containing ten layers of four crystals. The design of the detector is optimized for ultralow-background searches for neutrinoless double beta decay of 130Te (33.8% abundance), cold dark matter, solar axions, and rare nuclear decays. A preliminary experiment involving 20 crystals of various sizes (MIBETA) has been completed, and a single CUORE tower is being constructed as a smaller scale experiment called CUORICINO. The expected performance and sensitivity, based on Monte Carlo simulations and extrapolations of present results, are reported. 相似文献
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Spectrally resolved luminescence associated with the decay of bound multiexciton complexes in optically excited Ge:Ga is observed. This is the first reported observation of multiexciton complexes in p-type germanium. The observed spectra are consistent with the shell model for bound multiexciton complexes.No-phonon, TA, LA, and TO phonon assisted luminescence are observed. From these spectra, the energies of the LA, TA, and TO phonons in Ge:Ga are determined. 相似文献
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