Complexing effects in the solvent extraction of thallium(I) 2-mercaptobenzothiazole

Studies of the extraction of thallum(l) from aqueous solution in the absence and presence of aqueous complexing agents into chloroform containing 2-mercaptobenzothiazole are described. Fluoride, chloride, bromide, iodide, thiocyanair, cyanide, and sulfate were employed as aqueous complexing agents. A number of the participating species were identified and association constants are estimated.     

1,3-Diyl trapping reactions. Fundamental investigations with application to the synthesis of linearly fused tricyclopentanoids

A comparison of the inter- and intramolecular diyl trapping routes to linearly fused tricyclopentanoids is presented. In addition, several of the factors which are responsible for the stereoselectivity which is associated with the intramolecular process are examined and it is concluded that conformational rather than electronic (secondary orbital) factors play the dominant role. It is shown that gem methyl groups located on the acyclic chain which joins the diyl and diylophile (in reference to 32 and 35, but not to 47) have no practical effect upon the outcome of the trapping reaction. The intramolecular process is stereospecific with respect to diylophile geometry, and highly stereoselective with respect to the ring junction stereochemistry. Finally, an abortive attempt to synthesize the marine natural product Δ⁹⁽¹²)-capnellene (19) as well as a successful synthesis of the mold metabolite d,l- hirsutene (18) is presented.     

Diffusion-controlled reaction. V. Effect of concentration-dependent diffusion coefficient on reaction rate in graft polymerization

The effect of diffusion on radiation-initiated graft polymerization has been studied with emphasis on the single- and two-penetrant cases. When the physical properties of the penetrants are similar, the two-penetrant problem can be reduced to the single-penetrant problem by redefining the characteristic parameters of the system. The diffusion-free graft polymerization rate is assumed to be proportional to the v power of the monomer concentration C, in which the proportionality constant a = k_pR/k, where k_p and k_t are the propagation and termination rate constants, respectively, and R_i is the initiation rate. The values of v, w, and z depend on the particular reaction system. The results of our earlier work were generalized by allowing a non-Fickian diffusion rate, obtained from an extension of the Fujita free-volume theory, which predicts an essentially exponential dependence on the monomer concentration of the diffusion coefficient, D = D₀ [exp(δC/M)], where M is the saturation concentration. It was shown that a reaction system is characterized by the three dimensionless parameters v, δ, and A = (L/2)[aM^(v?1)/D₀]^1/2, where L is the polymer film thickness. Graft polymerization tends to become diffusion controlled as A increases. Larger values of δ and v cause a reaction system to behave closer to the diffusion-free regime. The transition from diffusion-free to diffusion-controlled reaction involves changes in the dependence of the reaction rate on film thickness, initiation rate, and monomer concentration. Although the diffusion-free rate is w order in initiation rate, v order in monomer, and independent of film thickness, the diffusion-controlled rate is w/2 order in initiator rate and inverse first-order in film thickness. The dependence of the diffusion-controlled rate on monomer is dependent in a complex manner on the diffusional characteristics of the reaction system.     

The growth of crystals in solution

The crystallization of sparingly soluble salts from their aqueous supersaturated solutions is discussed from the standpoint of two important applications; scale formation and biological mineralization. Theories of crystal growth are outlined and the importance of kinetic factors in determining the nature of the growing phases is discussed. The kinetic factors can be studied by using a highly reproducible seeded growth technique and under certain conditions secondary nucleation can also be induced on the surface of the inoculating seed crystals. The kinetics of crystallization of the alkaline earth surfaces and the calcium phosphates is discussed. In the latter systems, temperature, supersaturation, surface concentration, pH, ionic strength and the presence of foreign ions are important in determining the nature of the phase which grows on the added seed crystals. The mechanism of the retardation of crystal growth by added crystallization inhibitors is illustrated by the influence of organic phosphonate molecules upon the precipitation of calcium carbonate.     

Substitution and redox reactions involving vanadium oxotrifluorine

VOF₃ is soluble in acetonitrile but is slowly reduced to give a vanadyl (IV) species as one product. It reacts rapidly with Me₃SiNet₂ in MeCN below ambient temperature; fluorine is replaced by -NEt₂ ligands but the spectroscopic and magnetic properties of the products indicate that, in addition, reduction to V^IV occurs not some extent. The products are formulated as VOF_3-n(NEt₂)_n containing small quantities of VOF_2-m(NEt₂)_m(NEt₂H) (n = 1-3, m = 0-2). A similar reaction occurs between VOF₃ and Me₃SiOMe but with Me₃SiOSiMe₃ only VO₂F is formed.     

Approaches to phenazine-derived N-isosteres of anthracyclinones

1-Hydroxyphenazine 5,10-dioxide showed antitumor properties against mouse leukemia P388. It also participated in biochemical mechanisms of quinoid antitumor agents, as indicated by inhibition of radiolabeled DNA-RNA precursors in cultured leukemia L1210 cells and by stimulation of oxygen consumption in mammalian microsomes. This suggests that the isosteric di-N-oxide system may be a biologically active substitute for 1,4-quinone, and that di-N-oxides of tetrahydrobenzo[b]phenazines can be explored as anthracyclinone N-isosteres. As potential synthetic intermediates, 7,8,9,10-tetrahydro-6,11-dihydroxybenzo[b]-phenazines have been prepared by 1) Diels-Alder addition of phenazine-1,4-quinone and 1-methoxy-3-(trimethylsilyloxy)-1,3-butadiene to give isolable but labile adducts and 2) condensation of 6,7-diamino-1,2,3,4-tetrahydro-2-hydroxy-5,8-dimethoxy-2-naphthoic acid with 3-methoxy-1,2-quinone. Attempts at N-oxidation gave instead oxidation of the 6,11-hydroquinone ring to quinone, regardless of hydroxyl protection. Despite previous literature indications, we have been unable to synthesize the 1,4-dihydroxyphenazine 5,10-dioxide system. We conclude that this hydroxyl substitution pattern (1,4) in an adjacent ring must be avoided in the redesign of anthracyclinone isosteres that have di N-oxide in place of quinone.     

Immobilized enzyme electrode for the determination of salicylate in blood serum

This determination of salicylate in blood serum is based on application of an immobilized enzyme electrode. Salicylate hydroxylase (E.C.1.14.13.1) is chemically immobilized onto a pig intestine mounted on an oxygen electrode. The signals are monitored amperometrically and the resulting output voltage is read using a simple adapter. The experimental parameters and possible interferences are discussed. Samples containing 1.0 × 10^?5?1.87 × 10^?3 M (1.6–300 μg ml^?1) salicylate were assayed with relative standard deviations between 1.3% and 6% and recoveries between 98.7 and 103%. Results obtained by the proposed method and by the established clinical method for randomly spiked pooled serum samples correlated well (r = 0.99).     

Soft-laser scanning densitometry performed on a 35-mm slide illustrating SDS-PAGE separation of adenovirion polypeptides

Virion polypeptides (35 S), methionine-labeled and purified by CsCl gradient centrifugation, were separated by SDS-gel electrophoresis. Analysis of their band pattern was performed by scanning the images of the SDS-gels shown on a 35-mm slide. The densitometric tracings revealed the presence of 17 protein bands, although only 15 of them were visible to the naked eye. The high sensitivity and resolving capacities of the soft-laser scanning densitometer enabled us to detect trace amounts of protein bands separated in SDS-gels and to obtain a resolution compatible to that of electrophoresis. Fourfold electronic amplification of the densitometric tracings, produced by a computer, generated new information regarding the pattern of the electrophoregram. The facility to amplify peaks of importance is particularly advantageous when faint or overlapping protein bands revealed on a gel are assessed.